FastQCFastQC Report
Thu 29 Mar 2018
GFR-P4.r.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGFR-P4.r.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25868955
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT6222232.4052885012169996No Hit
GTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGAC2812021.0870249687318254No Hit
CGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC905630.3500837200420349No Hit
TGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC668840.25854929199884574No Hit
GCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTAT617030.23852142461881434No Hit
CGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA587480.22709846609575068No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA580790.22451235467377792No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC550700.21288065173100343Illumina Single End PCR Primer 1 (100% over 50bp)
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAGAGC536900.20754607211617165No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG532370.20579493837304214No Hit
TGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA467710.18079972693137392No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAT442370.17100420175457418No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAG369420.14280437690660483No Hit
GGACCTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCG356490.1378061077457516No Hit
AGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC300540.11617786648127068No Hit
GATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATG263920.1020219023149563No Hit
GCCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT261170.10095885202939198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA93150.067.739372
AGATCGG97050.065.3651051
ATCGGAA96450.065.18283
GTATACC1149050.058.51584694
CGTCTGG158200.056.982981
AGAGCGT112650.054.4836049
AAGAGCG116450.053.472998
CGTGTCT137350.045.39881
CGGAAGA143250.045.076675
TCGGAAG160250.040.8151254
CGCCGTA78400.037.67469846-47
CGTATCA80800.035.6297650-51
GTCTGGA1751450.035.4463461
GGAGTCT1988250.034.1717265
GAGTCTT1996600.034.077376
TGGAGTC1984850.033.842844
AGTCTTG2008700.033.801787
TCTGGAG1872550.033.43352
CTGGAGT2057250.032.2749023
GCCGTAT91450.031.63256348-49
Produced by FastQC (version 0.11.3)