FastQCFastQC Report
Thu 29 Mar 2018
GFR-S3.r.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGFR-S3.r.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21092959
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT3985821.8896447862056718No Hit
GTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGAC1628150.7718926491062729No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA818260.3879303989544568No Hit
GCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTAT573810.2720386456921478No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC522310.24762291530552918Illumina Single End PCR Primer 1 (100% over 50bp)
CGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC471650.2236054220747312No Hit
TGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC420830.19951207414758643No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG398250.18880708012564762No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAT384920.18248743573625684No Hit
CGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA371770.17625312788025616No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG332300.1575407224752108No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAGAGC307610.1458353946451989No Hit
TGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA288210.1366380127131523No Hit
TGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG279840.13266986391051158No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAG277350.13148937519861487No Hit
GGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG252140.11953751960547593No Hit
GATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATG244550.11593916244752574No Hit
CGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCA220400.1044898442176842No Hit
TCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTC219030.10384033838021493No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG216850.10280681814249013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA93150.069.407932
AGATCGG100150.064.4715351
ATCGGAA99450.064.404633
AGAGCGT111050.057.8012969
GTATACC768300.057.29848594
AAGAGCG115050.056.1180348
CGGAAGA136350.048.5275045
CGTCTGG99500.048.315311
TCGGAAG144900.046.2451134
CGTGTCT103300.039.6232451
CGCCGTA78700.038.21581646-47
CGTATCA82300.035.83763550-51
GTCTGGA1186400.033.97321
AACCTGG306000.033.10610294
GTCGCCG91950.032.86114544-45
GGAGTCT1357800.032.5094035
CCGTATC92750.032.48138448-49
GGTCGCC90900.032.46528644-45
GAGTCTT1363400.032.360866
GCCGTAT91000.032.33131448-49
Produced by FastQC (version 0.11.3)