FastQCFastQC Report
Thu 29 Mar 2018
GFR-S5.f.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGFR-S5.f.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26417955
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG11730354.440294489107881No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG2959171.1201359075674102No Hit
GGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA1047240.39641221283025124No Hit
ATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACC943580.3571737479301483No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA899600.3405259793954528No Hit
TGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA546860.20700315372631983No Hit
ATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAAT474120.17946884987880402No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATG412270.15605674246927895TruSeq Adapter, Index 9 (97% over 36bp)
GTATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG388560.14708178585359843No Hit
GGGAGAGATCCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTT361580.13686903471521544No Hit
TATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA278250.10532609356023205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT356750.084.355896
CGCGGTG373100.080.6719747
GGGCGCG378200.079.794924
GGGCGAT1509600.079.562649
AGGGCGA1544550.077.750438
GAGGGCG1572300.076.39917
CGGGCGC393650.076.0775453
GGCGCGG406050.074.53155
CCGGGCG396450.074.501232
CTCCCTC1525150.074.0259294
ATGTGAG1598750.073.392363
TGAGGGC1637700.073.327966
GTGAGGG1634500.073.322145
GATGTGA1593150.071.166062
TGTGAGG1709750.070.088634
GGATGTG1625000.069.8004761
CGGTGGC435350.069.2229169
GCGGTGG438800.068.796728
GCCGGGC469950.062.9135131
CTATGCC503200.059.7971394
Produced by FastQC (version 0.11.3)