FastQCFastQC Report
Thu 29 Mar 2018
GFR-S5.r.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGFR-S5.r.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26417955
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT4619921.748780327621877No Hit
GTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGAC1885930.7138819034251516No Hit
GCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTAT988600.37421518811732396No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA773510.29279707683656814No Hit
CGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC639700.24214591931888746No Hit
TGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC424780.16079215821209475No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC412840.15627250481727295Illumina Single End PCR Primer 1 (100% over 50bp)
CGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA404100.1529641488146982No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG365660.13841343889033045No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAGAGC316200.11969132357141195No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAT304520.1152700880897102No Hit
TGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGA293250.1110040500863901No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG269110.1018663253836264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA78900.063.8378642
GTATACC826950.061.33600294
AGATCGG89100.056.6966171
ATCGGAA88750.056.2280353
CGTCTGG131550.053.2971951
AGAGCGT97500.049.9285139
AAGAGCG105400.047.3453528
CGGAAGA125900.040.7215775
CGTGTCT113550.036.335721
GTCTGGA1300450.036.2690851
GGAGTCT1442900.035.222155
GAGTCTT1461200.034.8888476
AGTCTTG1459650.034.81777
TGGAGTC1454350.034.6708684
CGCCGTA65350.034.66079346-47
TCGGAAG150900.034.3462834
TCTGGAG1411550.033.5757982
CTGGAGT1522250.032.8591583
GTCTTGG1590600.032.085028
CGTATCA70900.031.19059450-51
Produced by FastQC (version 0.11.3)