FastQCFastQC Report
Thu 29 Mar 2018
GFR-S6.f.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGFR-S6.f.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25024279
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG9548313.815618424011337No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG1245530.49772862586770233TruSeq Adapter, Index 1 (100% over 49bp)
ATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACC1213010.48473324646036753No Hit
GGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA1114180.445239601108987No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG963930.3851979111965624No Hit
TGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA599640.23962328744816186No Hit
ATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAAT572870.22892567653997145No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA452550.18084437118048435No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCTAGCTACTCGGGAGGCTGAG375190.14993039359895244No Hit
GTATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG319420.12764403721681652No Hit
CCTATGTTCATTAATCGTTGATTATTCTCAACCAATCACAAAGATATCGG303130.12113435915576229No Hit
TATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA282180.11276248958061888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT183150.077.026486
GGGCGAT1318200.076.137379
AGGGCGA1345000.074.588688
GGGCGCG190250.073.860054
GAGGGCG1369700.073.1990057
GATCGGA232750.073.171112
AGATCGG235900.072.2092061
CTCCCTC1349150.070.2077794
ATGTGAG1400200.069.9536743
CGGGCGC199100.069.939013
GTGAGGG1435700.069.7716755
CGCGGTG202300.069.758557
TGAGGGC1440450.069.704586
GATGTGA1399500.067.776862
TGTGAGG1482200.067.6007464
GGATGTG1411800.067.189041
CCGGGCG203500.067.089382
GGCGCGG216150.065.206755
ATCGGAA296000.059.4718063
GCCGGGC243250.055.9986571
Produced by FastQC (version 0.11.3)