FastQCFastQC Report
Thu 29 Mar 2018
GFR-S7.r.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGFR-S7.r.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30727228
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT3933031.279982040683917No Hit
GCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTAT1519490.4945092996999274No Hit
GTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGAC1510230.49149568584579123No Hit
GGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCAC1200820.3907999771407951No Hit
GGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCC1131870.368360595365127No Hit
GTCCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC1101700.3585419420196316No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG1006680.327618228367362No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA984070.3202599336328028No Hit
CGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGA899280.29266551476755404No Hit
CTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGC880690.28661550596103236No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT755000.24571041683291445No Hit
CGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCG729770.2374994581353059No Hit
GGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCG704650.22932429830637507No Hit
GCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAG697280.22692577410497297No Hit
GTTCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC696880.22679559640069064No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG661910.21541481060380713No Hit
ATCCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTC638050.20764971054336562No Hit
TCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCC633200.2060713058789423No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC596730.19420235369100003No Hit
GTCCCGTTCCGTTCCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT562480.1830558877618248No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA540370.17586031515761852No Hit
GCCCTTCACAAAGAAAAGAGAACTCTCCCCGGGGCTCCCGCCGGCTTCTC539530.1755869419786256No Hit
GCGGGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTT531720.17304522230251293No Hit
GGCGGGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTT524580.1707215502810732No Hit
CGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC499990.16271887591031642No Hit
CCCCGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATA496150.16146916994920596No Hit
GTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGCGCCAG476900.15520436793061843No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGC464410.15113956911440238No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC444430.14463719278549955Illumina Single End PCR Primer 1 (100% over 50bp)
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT416960.13569723894390995No Hit
TCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTG408440.13292445384269613No Hit
GCCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCT394770.1284756307988472No Hit
GTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTC362560.11799307116151186No Hit
GCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCC345550.11245726428690542No Hit
GGGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAG339110.11036140324795975No Hit
GGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATT329360.10718832170607775No Hit
ACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG327110.10645607211948958No Hit
CCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA326750.1063389121856355No Hit
GCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGC314910.10248565213887827No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG313890.1021536989929583No Hit
GGGTCTTCCGTACGCCACATTTCCCACGCCGCGACGCGCGGCGGGGATTC312000.10153860934022424No Hit
TGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACC309390.100689199819782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA80100.067.107452
AGATCGG85650.062.9313431
GTATACC704550.060.28125494
AGAGCGT94450.055.6705939
AGTTTAC240200.054.02868394
CGTCTGG100650.050.1442831
ATCGGAA111650.047.8543473
CGGAAGA117800.046.7153435
GTCCCGA69550.044.729271
GTCCCGT436800.044.486051
GGGCGGT316350.044.3857651
TTCGCGC126950.043.491288
TCCCGTT495100.042.8681142
TCGCGCC130450.042.432539
TTACGAC41550.042.4069942
CTTCGCG131550.042.152937
CGCTTCG128850.041.2502065
GTACGTA69100.039.852041
TCGGAAG139750.039.610434
GTCTGGA1031700.038.9032821
Produced by FastQC (version 0.11.3)