FastQCFastQC Report
Thu 29 Mar 2018
GFR-S8.f.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGFR-S8.f.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26113252
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG11881484.549980982835841No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG2567480.983209597946667No Hit
GGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA1227120.4699223214328112No Hit
ATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACC1014990.38868770538422404No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCTAGCTACTCGGGAGGCTGAG829770.3177582018509223No Hit
TGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA645810.24731121194709874No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA460130.17620555264430487No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG423060.16200969530719497TruSeq Adapter, Index 3 (100% over 49bp)
ATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAAT393670.15075487342595245No Hit
GTATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG364160.139454097865712No Hit
CCTATGTTCATTAATCGTTGATTATTCTCAACCAATCACAAAGATATCGG329670.12624624462705755No Hit
TGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGG271060.10380170190981959No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT395300.084.178416
GGGCGCG414800.080.266964
CGCGGTG415150.080.221737
CGGGCGC421150.078.497723
GGGCGAT1568200.077.660529
AGGGCGA1597250.076.245288
CCGGGCG426900.076.079142
GAGGGCG1616450.075.3222057
GGCGCGG448950.074.287955
CTCCCTC1569950.072.7121794
ATGTGAG1643300.072.6376343
TGAGGGC1685300.072.255926
GTGAGGG1693900.071.818865
GATGTGA1647200.070.182752
TGTGAGG1747250.069.5847854
GCGGTGG480800.069.346188
CGGTGGC482100.069.090839
GGATGTG1676650.068.965921
GCCGGGC489200.066.299571
CTATGCC538350.061.593394
Produced by FastQC (version 0.11.3)