FastQCFastQC Report
Thu 29 Mar 2018
GFR-S9.f.slx.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameGFR-S9.f.slx.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24320429
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG11722724.820112342590667No Hit
GGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA1957920.8050515885225545No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG1641440.6749223050300633No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCTAGCTACTCGGGAGGCTGAG1566990.6443101805482132No Hit
ATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAGTCCATGACC1153910.4744612029664444No Hit
TGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA1028610.4229407301984681No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG572040.2352096667373754TruSeq Adapter, Index 4 (100% over 49bp)
ATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAAT486690.20011571341936443No Hit
ACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAA450480.18522699579024696No Hit
CCTATGTTCATTAATCGTTGATTATTCTCAACCAATCACAAAGATATCGG423970.1743266946483551No Hit
GTATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAG392270.1612923850973188No Hit
CGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCA344870.14180259731438125No Hit
TCAACATGATTTATCACAATTATCTCATCAATAATTACCCTATTTATCTT254870.10479667114424668No Hit
TATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTA246790.10147436132808348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT386250.082.378916
GGGCGCG404300.078.530474
CGCGGTG405950.078.427677
CGGGCGC407200.077.28943
CCGGGCG405200.076.351752
CTCCCTC1496100.075.01552694
GGCGCGG429400.074.116285
GGGCGAT1668200.071.5766149
AGGGCGA1697300.070.335748
GAGGGCG1724950.069.2055747
GCGGTGG464400.068.657858
CGGTGGC465000.068.569129
GCCGGGC452950.068.258861
ATGTGAG1735300.067.419483
GTGAGGG1784800.066.74995
TGAGGGC1792400.066.590676
TGTGAGG1828550.065.154614
GATGTGA1736600.064.988872
GGATGTG1763650.064.0502241
GATCGGA135250.063.864872
Produced by FastQC (version 0.11.3)