Experiment Volunteer CD4/CD8 Subset # cells (millions) # reads alpha (millions) # UMIs alpha # reads beta (millions) # UMIs beta 1 1 CD4 Naive 1.82 1.04 47570 1.06 108648 1 1 CD4 CM 8.7 0.99 49404 1.27 89102 1 1 CD4 EM 1.54 1.16 81799 1.12 150498 1 1 CD4 EMRA 0.2 2.59 46356 2.59 99641 1 1 CD8 Naive 0.84 1.2 27182 1.34 56290 1 1 CD8 CM 0.56 1.12 82937 1.26 151414 1 1 CD8 EM 0.47 0.92 47261 1.13 107653 1 1 CD8 EMRA 2 1.17 105603 1.43 198784 1 1 CD4 Naive 5.9 1.46 32871 1.33 62178 1 2 CD4 CM 5.2 1.19 59981 3 105663 1 2 CD4 EM 2.65 1.32 72447 1.46 125186 1 2 CD4 EMRA 0.26 1.39 18646 1.44 52385 1 2 CD8 Naive 4.1 1.57 37746 1.5 78853 1 2 CD8 CM 2.3 0.89 44863 1.13 79697 1 2 CD8 EM 1.5 1.02 34471 0.99 65215 1 2 CD8 EMRA 1.6 2.02 141997 2.02 227364 2 3 CD4 Naive-1 3.23 1.65 74755 2.02 141605 2 3 CD4 Naive-2 3.23 1.59 93198 1.24 147658 2 3 CD4 Naive-3 3.23 1.57 67859 1.3 141847 2 3 CD4 CM 9.7 1.69 159185 1.46 203741 2 3 CD4 EM 2.7 1.98 74507 1.15 135283 2 3 CD4 EMRA 0.21 1.24 14845 2.05 15120 2 3 CD8 Naive-1 1.67 1.61 69854 1.62 140621 2 3 CD8 Naive-2 1.67 1.69 69151 1.69 146557 2 3 CD8 Naive-3 1.67 1.75 72281 1.57 135547 2 3 CD8 CM 2.6 1.21 63031 2 12188 2 3 CD8 EM 0.46 1.33 66676 1.78 112139 2 3 CD8 EMRA 0.47 1.27 59932 1.78 116176