run("Split Channels");
setAutoThreshold("Huang dark");
run("Create Selection");
roiManager("Add");
close();
setAutoThreshold("Triangle dark");
setOption("BlackBackground", false);
run("Convert to Mask");
roiManager("Select", 0);
run("Fill", "slice");
run("Select None");
run("Analyze Particles...", "size=10-Infinity pixel show=Outlines display summarize");
roiManager("Delete");
close();
close();