Figure 2 - Source Data 1 Model 1 parameters during stable period for example mice (each data is the median value of corresponding parameter distribution, each row contains data from one animal) ¦Á1 ¦Á2 ¦Â ¦Ã1 ¦Ã2 -0.180811487123034 -0.0814277979863749 -0.111551128110809 3.74702579780239 -0.028491589796249 0.0395144418514156 0.188521235329382 0.182398603904979 3.43073664705806 0.415933376803646 -0.0798018892649647 0.0613681074899526 -0.0412012428927988 3.24385281200519 -0.0123599562107701 -0.178015557261793 0.144795629367217 0.0060359621259146 3.56592543252267 0.178634509489895 -0.0279298286357967 0.821743506677735 0.296363178272405 4.62270117587258 0.791509724136437 -0.161425200447349 0.177266181309786 -0.00963975715624856 4.41019465199601 0.117432469758882 0.0457453404876107 0.447888821296072 0.152117517175971 5.07900084537675 0.400825905835092 0.062597334781035 0.0250724370803043 -0.019910781329367 4.21936828832938 0.0382336440671995 -0.248013928038602 0.100228406108171 0.0532849364274816 4.75721718899661 0.437448289580818 0.105010556275168 0.203084183851864 -0.0236860187967394 4.74113228104867 -0.100406665104876 __________ Statistics ¦Á1 ¦Á2 ¦Â ¦Ã1 ¦Ã2 Number of values 10 10 10 10 10 Minimum -0.248 -0.08143 -0.1116 3.244 -0.1004 25% Percentile -0.1787 0.05229 -0.02806 3.532 -0.01639 Median -0.05387 0.161 -0.001802 4.315 0.148 75% Percentile 0.04996 0.2643 0.1597 4.745 0.4213 Maximum 0.105 0.8217 0.2964 5.079 0.7915 Mean -0.06231 0.2089 0.04842 4.182 0.2239 Std. Deviation 0.1242 0.256 0.1242 0.642 0.2806 Std. Error of Mean 0.03928 0.08096 0.03928 0.203 0.08873 Lower 95% CI of mean -0.1512 0.02571 -0.04044 3.722 0.02316 Upper 95% CI of mean 0.02655 0.392 0.1373 4.641 0.4246 Sum -0.6231 2.089 0.4842 41.82 2.239 Wilcoxon Signed Rank Test Theoretical median 0 0 0 0 0 Actual median -0.05387 0.161 -0.001802 4.315 0.148 Discrepancy -0.05387 0.161 -0.001802 4.315 0.148 Sum of signed ranks (W) -25 49 13 55 41 Sum of positive ranks 15 52 34 55 48 Sum of negative ranks -40 -3 -21 0 -7 P value (two tailed) 0.2324 0.0098 0.5566 0.0020 0.0371 Exact or estimate? Exact Exact Exact Exact Exact Significant (alpha=0.05)? No Yes No Yes Yes ________________________________________________________________________________________________________________________________________________________ Model 1 parameters during switching period for example mice (each data is the median value of corresponding parameter distribution, each row contains data from one animal) ¦Á1 ¦Á2 ¦Â ¦Ã1 ¦Ã2 0.17095562802471 -0.156670213566274 0.104127820772919 3.75123805690822 0.245249660182304 -0.16890945617326 0.246665021988644 0.262539526332381 3.61985306912048 0.0952790443939549 -0.0973645496381561 -0.263169764736898 0.280058482038014 3.11238932813615 0.366156670390999 -0.17783987869642 -0.0278333684915397 0.123561730281988 3.28827637458362 0.379130800487403 0.0779650948056022 0.0863462873193768 0.256763228610773 4.17253641968982 0.400809402520408 0.0941378911720357 0.260045620396478 0.0846747289802956 4.03905472361174 0.0882217997008221 -0.123106158169482 -0.286474241921411 0.297067127269372 4.09835315458536 0.427674761801217 0.207129805734253 -0.112367180479046 0.0810370363196179 3.88526213455576 0.145762165321329 -0.198882345049688 -0.0742768556125232 0.211189030938702 4.26928700826582 0.379791892675585 -0.148922200201544 0.11301105891588 0.482044371836616 3.64141678904376 0.148269987350804 __________ Statistics ¦Á1 ¦Á2 ¦Â ¦Ã1 ¦Ã2 Number of values 10 10 10 10 10 Minimum -0.1989 -0.2865 0.08104 3.112 0.08822 25% Percentile -0.1711 -0.1833 0.09926 3.537 0.1331 Median -0.1102 -0.05106 0.234 3.818 0.3057 75% Percentile 0.1133 0.1464 0.2843 4.117 0.385 Maximum 0.2071 0.26 0.482 4.269 0.4277 Mean -0.03648 -0.02147 0.2183 3.788 0.2676 Std. Deviation 0.1564 0.194 0.1255 0.3809 0.1373 Std. Error of Mean 0.04947 0.06136 0.03969 0.1204 0.04341 Lower 95% CI of mean -0.1484 -0.1603 0.1285 3.515 0.1694 Upper 95% CI of mean 0.07543 0.1173 0.3081 4.06 0.3658 Sum -0.3648 -0.2147 2.183 37.88 2.676 Wilcoxon Signed Rank Test Theoretical median 0 0 0 0 0 Actual median -0.1102 -0.05106 0.234 3.818 0.3057 Discrepancy -0.1102 -0.05106 0.234 3.818 0.3057 Sum of signed ranks (W) -15 -9 55 55 55 Sum of positive ranks 20 23 55 55 55 Sum of negative ranks -35 -32 0 0 0 P value (two tailed) 0.4922 0.6953 0.0020 0.0020 0.0020 Exact or estimate? Exact Exact Exact Exact Exact Significant (alpha=0.05)? No No Yes Yes Yes _________________________________________________________________ Stable period vs switching period parameters for example mice ___________________ Test results for ¦Á1 Column B Stable vs. vs. Column A Switching Wilcoxon matched-pairs signed rank test P value 0.6953 Exact or approximate P value? Exact P value summary ns Significantly different (P < 0.05)? No One- or two-tailed P value? Two-tailed Sum of positive, negative ranks 23 , -32 Sum of signed ranks (W) -9 Number of pairs 10 Median of differences Median -0.02465 How effective was the pairing? rs (Spearman) 0.2121 P value (one tailed) 0.2801 P value summary ns Was the pairing significantly effective? No ___________________ Test results for ¦Á2 Column B Stable vs. vs. Column A Switching Wilcoxon matched-pairs signed rank test P value 0.0137 Exact or approximate P value? Exact P value summary * Significantly different (P < 0.05)? Yes One- or two-tailed P value? Two-tailed Sum of positive, negative ranks 51 , -4 Sum of signed ranks (W) 47 Number of pairs 10 Median of differences Median 0.155 How effective was the pairing? rs (Spearman) 0.3697 P value (one tailed) 0.1478 P value summary ns Was the pairing significantly effective? No ___________________ Test results for ¦Â Column B Stable vs. vs. Column A Switching Wilcoxon matched-pairs signed rank test P value 0.0039 Exact or approximate P value? Exact P value summary ** Significantly different (P < 0.05)? Yes One- or two-tailed P value? Two-tailed Sum of positive, negative ranks 1 , -54 Sum of signed ranks (W) -53 Number of pairs 10 Median of differences Median -0.1312 How effective was the pairing? rs (Spearman) 0.1758 P value (one tailed) 0.3161 P value summary ns Was the pairing significantly effective? No ___________________ Test results for ¦Ã1 Column B Stable vs. vs. Column A Switching Wilcoxon matched-pairs signed rank test P value 0.0137 Exact or approximate P value? Exact P value summary * Significantly different (P < 0.05)? Yes One- or two-tailed P value? Two-tailed Sum of positive, negative ranks 51 , -4 Sum of signed ranks (W) 47 Number of pairs 10 Median of differences Median 0.3526 How effective was the pairing? rs (Spearman) 0.8182 P value (one tailed) 0.0029 P value summary ** Was the pairing significantly effective? Yes ___________________ Test results for ¦Ã2 Column B Stable vs. vs. Column A Switching Wilcoxon matched-pairs signed rank test P value 0.6953 Exact or approximate P value? Exact P value summary ns Significantly different (P < 0.05)? No One- or two-tailed P value? Two-tailed Sum of positive, negative ranks 23 , -32 Sum of signed ranks (W) -9 Number of pairs 10 Median of differences Median -0.06719 How effective was the pairing? rs (Spearman) 0.4424 P value (one tailed) 0.1022 P value summary ns Was the pairing significantly effective? No