Figure 4 - Source Data 1 Data related to bilateral DREADD inhibition of M2. ____________________________________________________ Mean percent correct for reversing stimulus Period Saline Saline Saline Saline Saline Saline Saline Saline Saline Saline CNO CNO CNO CNO CNO CNO CNO CNO CNO CNO Stable 81.6419860627178 78.9498761196875 84.1543513957307 71.1688311688312 86.5672696438666 79.872311827957 80.9654631083203 82.7464788732394 80.2197802197802 82.5231481481482 79.9873882137125 80.02800280028 83.151904997526 76.9503546099291 81.8038686459739 78.8803240135877 82.53367003367 82.4056895485467 79.4111394557823 77.5655864197531 Switching 50.6386 49.12165 47.49045 52.8946 57.01005 52.28265 53.68835 52.7796 56.54685 52.6265 41.8725 37.7848 49.13685 56.684 53.6885 53.87435 45.15595 45.6151 50.07895 50.45455 ___________________ 2 way ANOVA results Two-way RM ANOVA Matching: Both factors Alpha 0.05 Source of Variation % of total variation P value P value summary Significant? Period 93.2 <0.0001 **** Yes Manipulation 0.5637 0.0441 * Yes Interaction: Period x Manipulation 0.3085 0.0870 ns No Interaction: Period x Subjects 2.541 Interaction: Manipulation x Subjects 0.9278 Subjects 1.711 ANOVA table SS DF MS F (DFn, DFd) P value Period 9063 1 9063 F (1, 9) = 330.1 P<0.0001 Manipulation 54.82 1 54.82 F (1, 9) = 5.468 P=0.0441 Interaction: Period x Manipulation 30 1 30 F (1, 9) = 3.689 P=0.0870 Interaction: Period x Subjects 247.1 9 27.46 Interaction: Manipulation x Subjects 90.23 9 10.03 Subjects 166.4 9 18.49 Residual 73.2 9 8.134 ___________________________ Multiple comparison results Compare each cell mean with the other cell mean in that row Number of families 1 Number of comparisons per family 2 Alpha 0.05 Sidak's multiple comparisons test Mean Diff. 95.00% CI of diff. Significant? Summary Adjusted P Value Saline - CNO Stable 0.6092 -2.805 to 4.024 No ns 0.8735 Switching 4.073 0.6588 to 7.488 Yes * 0.0218 Test details Mean 1 Mean 2 Mean Diff. SE of diff. N1 N2 t DF Saline - CNO Stable 80.88 80.27 0.6092 1.275 10 10 0.4776 9 Switching 52.51 48.43 4.073 1.275 10 10 3.194 9 __________________________________________________________________________________________________________________ Mean response latency for reversing stimulus(in ms) Period Saline Saline Saline Saline Saline Saline Saline Saline Saline Saline CNO CNO CNO CNO CNO CNO CNO CNO CNO CNO Stable 1483.57288786482 1516.35293040293 1405.50982142857 1816.9480952381 1726.69502164502 1674.49652777778 1464.01696428571 1452.95320294785 1781.03246753247 1599.76666666667 1463.29927536232 1479.1184981685 1389.37101449275 1748.45452380952 1723.79962797619 1539.85681818182 1366.23513708514 1499.63554421769 1806.52695238095 1660.23988095238 Switching 1491.09236874237 1751.73480392157 1464.79191919192 1900.95022675737 1787.87639880952 1655.8248015873 1652.80303030303 1700.82236227824 1947.32173579109 1838.73011583012 1436.43005952381 1553.91525857655 1444.80130208333 1850.20898268398 1848.18855311355 1564.16912698413 1560.31768292683 1653.45157142857 1927.19992319508 1664.02946127946 ___________________ 2 way ANOVA results Two-way RM ANOVA Matching: Both factors Alpha 0.05 Source of Variation % of total variation P value P value summary Significant? Period 10.42 0.0013 ** Yes Manipulation 2.068 0.0174 * Yes Interaction: Period x Manipulation 0.4682 0.1694 ns No Interaction: Period x Subjects 4.464 Interaction: Manipulation x Subjects 2.203 Subjects 78.49 ANOVA table SS DF MS F (DFn, DFd) P value Period 109912 1 109912 F (1, 9) = 21.02 P=0.0013 Manipulation 21811 1 21811 F (1, 9) = 8.449 P=0.0174 Interaction: Period x Manipulation 4938 1 4938 F (1, 9) = 2.232 P=0.1694 Interaction: Period x Subjects 47071 9 5230 Interaction: Manipulation x Subjects 23232 9 2581 Subjects 827708 9 91968 Residual 19913 9 2213 ___________________________ Multiple comparison results Compare each cell mean with the other cell mean in that row Number of families 1 Number of comparisons per family 2 Alpha 0.05 Sidak's multiple comparisons test Mean Diff. 95.00% CI of diff. Significant? Summary Adjusted P Value Saline - CNO Stable 24.48 -31.84 to 80.8 No ns 0.4736 Switching 68.92 12.61 to 125.2 Yes * 0.0191 Test details Mean 1 Mean 2 Mean Diff. SE of diff. N1 N2 t DF Saline - CNO Stable 1592 1568 24.48 21.04 10 10 1.164 9 Switching 1719 1650 68.92 21.04 10 10 3.276 9 _______________________________________________________________________________________________________________ Model 1 parameters during stable period, for saline/CNO sessions(each row contains data from one animal) __________________ ¦Á1, stable period Saline CNO 0.124011535494523 0.0882580330520658 -0.0372296819293979 -0.0127197877743288 -0.0700768342637919 -0.103316713302463 -0.342059804731515 0.0861816203720035 -0.176203381244838 -0.254749677492638 -0.0599689132153718 -0.17659604215661 -0.169885506234495 -0.21740949692171 0.250360007162715 -0.0439526848590363 -0.248427850331396 -0.489196425524063 0.175827300750654 0.194476085738256 ___________________ Test results for ¦Á1 Column B CNO vs. vs. Column A Saline Wilcoxon matched-pairs signed rank test P value 0.1602 Exact or approximate P value? Exact P value summary ns Significantly different (P < 0.05)? No One- or two-tailed P value? Two-tailed Sum of positive, negative ranks 13 , -42 Sum of signed ranks (W) -29 Number of pairs 10 Median of differences Median -0.04164 How effective was the pairing? rs (Spearman) 0.5515 P value (one tailed) 0.0524 P value summary ns Was the pairing significantly effective? No __________________ ¦Á2, stable period Saline CNO 0.110684281047128 0.339946099060691 0.642696577549826 0.267315399350623 0.534395063550903 0.588958343078407 0.2624921068174 -0.0886315220533671 0.278085907838804 0.547414344404993 0.123044668474944 0.0351059717545136 0.240901039820901 0.0994427132310118 1.43980863447452 0.687528209183159 0.497208065469504 0.327709905062739 0.911874994642401 0.472188366642316 ___________________ Test results for ¦Á2 Column B CNO vs. vs. Column A Saline Wilcoxon matched-pairs signed rank test P value 0.1309 Exact or approximate P value? Exact P value summary ns Significantly different (P < 0.05)? No One- or two-tailed P value? Two-tailed Sum of positive, negative ranks 12 , -43 Sum of signed ranks (W) -31 Number of pairs 10 Median of differences Median -0.1555 How effective was the pairing? rs (Spearman) 0.5879 P value (one tailed) 0.0403 P value summary * Was the pairing significantly effective? Yes __________________ ¦Â, stable period Saline CNO 0.0259173521321527 0.0313807698908039 0.0899968994886314 0.126254771244048 0.000544528763968269 0.186484502961658 0.0383506282314232 0.160521226536869 0.117626589814614 0.118411102245732 -0.0325094807809639 -0.109802346562105 0.181950433085434 -0.0466716458572466 0.14350276764387 0.0926667599576332 0.242215986956862 0.203288900220987 0.175155978732152 0.517330812912658 ___________________ Test results for ¦Â Column B CNO vs. vs. Column A Saline Wilcoxon matched-pairs signed rank test P value 0.7695 Exact or approximate P value? Exact P value summary ns Significantly different (P < 0.05)? No One- or two-tailed P value? Two-tailed Sum of positive, negative ranks 31 , -24 Sum of signed ranks (W) 7 Number of pairs 10 Median of differences Median 0.003124 How effective was the pairing? rs (Spearman) 0.3333 P value (one tailed) 0.1744 P value summary ns Was the pairing significantly effective? No __________________ ¦Ã1, stable period Saline CNO 3.24690416891015 3.60406692100054 3.66041125913051 3.47903627808908 4.25369058827115 4.21069101264995 6.03126738783521 3.85211649602861 5.43672406206582 4.7560375278073 4.90176089642235 3.78036659727862 4.24352574587797 4.74756515403122 6.08977233633978 5.15713368770663 4.54627288938741 4.37610379314409 4.47621297330754 3.47050410655507 ___________________ Test results for ¦Ã1 Column B CNO vs. vs. Column A Saline Wilcoxon matched-pairs signed rank test P value 0.0645 Exact or approximate P value? Exact P value summary ns Significantly different (P < 0.05)? No One- or two-tailed P value? Two-tailed Sum of positive, negative ranks 9 , -46 Sum of signed ranks (W) -37 Number of pairs 10 Median of differences Median -0.431 How effective was the pairing? rs (Spearman) 0.5394 P value (one tailed) 0.0569 P value summary ns Was the pairing significantly effective? No __________________ ¦Ã2, stable period Saline CNO 0.144171126322482 0.126438171033563 0.413519422073084 0.388508924405434 0.125988701366347 0.654298878050862 0.353556372637152 0.427294885939811 0.713605750419951 0.555772304029654 0.0706561962928921 -0.0160697818954269 0.709902346939906 0.21678646510307 0.351814960735116 0.395220870387911 0.887777958981945 0.969166790881458 0.388408003814219 1.06927407364315 ___________________ Test results for ¦Ã2 Column B CNO vs. vs. Column A Saline Wilcoxon matched-pairs signed rank test P value 0.7695 Exact or approximate P value? Exact P value summary ns Significantly different (P < 0.05)? No One- or two-tailed P value? Two-tailed Sum of positive, negative ranks 31 , -24 Sum of signed ranks (W) 7 Number of pairs 10 Median of differences Median 0.01284 How effective was the pairing? rs (Spearman) 0.4303 P value (one tailed) 0.1091 P value summary ns Was the pairing significantly effective? No _______________________________________________________________________________________________________________ Model 4 parameters during stable period, for saline/CNO sessions(each row contains data from one animal) ________________ ¦Â, stable period Saline CNO -0.014292855431624 -0.0217818448263881 -0.000204661117426931 0.120584767494944 0.0317668742057153 0.165115741746776 0.104387746330424 0.10895499324449 0.139556409967649 0.144537046358729 -0.028606149857221 -0.0596049874056697 0.213461357611906 0.00217310216467111 0.0305283362020176 0.0881986468254487 0.276817273994284 0.306876424290878 -0.0631685966477347 0.294182828173887 ___________________ Test results for ¦Â Column B CNO vs. vs. Column A Saline Wilcoxon matched-pairs signed rank test P value 0.3223 Exact or approximate P value? Exact P value summary ns Significantly different (P < 0.05)? No One- or two-tailed P value? Two-tailed Sum of positive, negative ranks 38 , -17 Sum of signed ranks (W) 21 Number of pairs 10 Median of differences Median 0.01752 How effective was the pairing? rs (Spearman) 0.297 P value (one tailed) 0.2035 P value summary ns Was the pairing significantly effective? No ________________ ¦Ã1, stable period Saline CNO 3.20223859806431 3.50735564735495 3.5627237796377 3.45230197275101 4.09786618937082 4.1441119755265 5.6408051401855 3.868504731065 5.49398119529064 4.73734013431476 4.93336154989992 3.76775531050624 4.29584283953871 4.77146184934297 5.71880841797551 4.97077709304055 4.45207393498481 4.41789166040431 4.41581856270468 3.39935957366892 ___________________ Test results for ¦Ã1 Column B CNO vs. vs. Column A Saline Wilcoxon matched-pairs signed rank test P value 0.1055 Exact or approximate P value? Exact P value summary ns Significantly different (P < 0.05)? No One- or two-tailed P value? Two-tailed Sum of positive, negative ranks 11 , -44 Sum of signed ranks (W) -33 Number of pairs 10 Median of differences Median -0.4292 How effective was the pairing? rs (Spearman) 0.5152 P value (one tailed) 0.0667 P value summary ns Was the pairing significantly effective? No ________________ ¦Ã2, stable period Saline CNO 0.119687212458853 0.0972717709777323 0.191893419967344 0.408899272017408 0.226334817864095 0.634938615422925 0.323280962235916 0.351671060363023 0.701532800995365 0.553217837202953 0.0688818432621254 -0.00418113211408358 0.731996411228658 0.214193644908207 0.307582235294938 0.424678100078131 0.883200235558981 0.992688956889821 -0.0278862219574442 0.772917629457168 ___________________ Test results for ¦Ã2 Column B CNO vs. vs. Column A Saline Wilcoxon matched-pairs signed rank test P value 0.4316 Exact or approximate P value? Exact P value summary ns Significantly different (P < 0.05)? No One- or two-tailed P value? Two-tailed Sum of positive, negative ranks 36 , -19 Sum of signed ranks (W) 17 Number of pairs 10 Median of differences Median 0.06894 How effective was the pairing? rs (Spearman) 0.2606 P value (one tailed) 0.2349 P value summary ns Was the pairing significantly effective? No _______________________________________________________________________________________________________________ Model 1 parameters during switching period, for saline/CNO sessions(each row contains data from one animal) __________________ ¦Á1, switching period Saline CNO 0.00321662045984871 0.473746885637034 -0.194720416852104 0.47537244714394 -0.198040995886694 -0.176571998086992 -0.0222514808660019 0.608810961109263 -0.580422505281754 -0.0877404220933709 -0.409863574265644 0.339290395798091 -0.148774445794279 -0.322272093642966 0.130014805343776 -0.419166837455136 -0.323110613284268 0.124503812615201 -0.07198896900855 0.412278055306911 ___________________ Test results for ¦Á1 Column B CNO vs. vs. Column A Saline Wilcoxon matched-pairs signed rank test P value 0.0645 Exact or approximate P value? Exact P value summary ns Significantly different (P < 0.05)? No One- or two-tailed P value? Two-tailed Sum of positive, negative ranks 46 , -9 Sum of signed ranks (W) 37 Number of pairs 10 Median of differences Median 0.4774 How effective was the pairing? rs (Spearman) 0.103 P value (one tailed) 0.3925 P value summary ns Was the pairing significantly effective? No __________________ ¦Á2, switching period Saline CNO -0.0696169562060661 -0.0953475054318384 0.0370755732265046 0.087769472537396 0.111378706706415 -0.0567237725501658 0.206008435280993 -0.283890334746834 0.326364086826093 0.045548973094767 -0.0696998286643916 -0.432299297417586 0.250897760704755 0.122813201086138 -0.0109180518862347 -0.172251179566556 0.0813059637365103 -0.137134897463986 -0.0279090961329138 0.205020171231458 ___________________ Test results for ¦Á2 Column B CNO vs. vs. Column A Saline Wilcoxon matched-pairs signed rank test P value 0.0645 Exact or approximate P value? Exact P value summary ns Significantly different (P < 0.05)? No One- or two-tailed P value? Two-tailed Sum of positive, negative ranks 9 , -46 Sum of signed ranks (W) -37 Number of pairs 10 Median of differences Median -0.1647 How effective was the pairing? rs (Spearman) 0.2848 P value (one tailed) 0.2135 P value summary ns Was the pairing significantly effective? No __________________ ¦Â, switching period Saline CNO 0.330257821686115 0.214432142219459 0.525476033846273 0.0194352727823235 0.0999661963167093 0.271446215985743 0.383404764233356 0.208609314984256 0.360335186360722 0.520998434050106 0.542362971685511 0.317560010314057 0.193164611110143 0.863075447100547 0.269680747357678 0.335820280365474 0.393982202577919 0.250939064678467 0.70043288919399 0.079663690398402 ___________________ Test results for ¦Â Column B CNO vs. vs. Column A Saline Wilcoxon matched-pairs signed rank test P value 0.4922 Exact or approximate P value? Exact P value summary ns Significantly different (P < 0.05)? No One- or two-tailed P value? Two-tailed Sum of positive, negative ranks 20 , -35 Sum of signed ranks (W) -15 Number of pairs 10 Median of differences Median -0.1294 How effective was the pairing? rs (Spearman) -0.5758 P value (one tailed) 0.0441 P value summary * Was the pairing significantly effective? Yes __________________ ¦Ã1, switching period Saline CNO 2.32080638058374 3.27327165970913 2.69881466168548 2.31593650196742 3.64058426401931 3.79798566519693 19.1402548771174 4.21081071433224 4.45557590996107 4.63255401052534 3.32715966687445 3.81451365716401 3.55118008156477 3.69037819095125 4.24596113870077 3.50725672881655 5.08250117073672 4.82479965635305 3.55876580848129 2.76824286917258 ___________________ Test results for ¦Ã1 Column B CNO vs. vs. Column A Saline Wilcoxon matched-pairs signed rank test P value 0.5566 Exact or approximate P value? Exact P value summary ns Significantly different (P < 0.05)? No One- or two-tailed P value? Two-tailed Sum of positive, negative ranks 21 , -34 Sum of signed ranks (W) -13 Number of pairs 10 Median of differences Median -0.05925 How effective was the pairing? rs (Spearman) 0.7212 P value (one tailed) 0.0117 P value summary * Was the pairing significantly effective? Yes __________________ ¦Ã2, switching period Saline CNO 0.230170646236604 -0.0771876660028076 0.235299697685787 -0.060064663602492 0.36662957254931 0.384830286811676 0.353648126572062 0.238169269779896 0.815755192911634 0.605179417624465 0.516319914131133 0.453547702505427 0.394399147292527 0.0990844299283567 0.361991341763237 0.524095942210315 0.790063164674324 0.323880076993866 0.57995320516707 -0.0113429267758801 ___________________ Test results for ¦Ã2 Column B CNO vs. vs. Column A Saline Wilcoxon matched-pairs signed rank test P value 0.0195 Exact or approximate P value? Exact P value summary * Significantly different (P < 0.05)? Yes One- or two-tailed P value? Two-tailed Sum of positive, negative ranks 5 , -50 Sum of signed ranks (W) -45 Number of pairs 10 Median of differences Median -0.2529 How effective was the pairing? rs (Spearman) 0.5636 P value (one tailed) 0.0481 P value summary * Was the pairing significantly effective? Yes _______________________________________________________________________________________________________________ Model 4 parameters during switching period, for saline/CNO sessions(each row contains data from one animal) __________________ ¦Â, switching period Saline CNO 0.331562954055761 0.288448329723164 0.393383201756877 0.220388512815913 0.0883893526975968 0.254035876408533 0.463813260518781 0.32091992117355 0.483940322155214 0.52311311310617 0.420682805953626 0.438201083912891 0.196275796677017 0.842767554644432 0.25934167054873 0.241578880159728 0.263971533083279 0.294333115838423 0.714865320660248 0.274431548132954 ___________________ Test results for ¦Â Column B CNO vs. vs. Column A Saline Wilcoxon matched-pairs signed rank test P value 0.8457 Exact or approximate P value? Exact P value summary ns Significantly different (P < 0.05)? No One- or two-tailed P value? Two-tailed Sum of positive, negative ranks 25 , -30 Sum of signed ranks (W) -5 Number of pairs 10 Median of differences Median -0.0001223 How effective was the pairing? rs (Spearman) 0.1758 P value (one tailed) 0.3161 P value summary ns Was the pairing significantly effective? No __________________ ¦Ã1, switching period Saline CNO 2.35001860656359 3.02962737459407 2.7467305043704 1.99085376798909 3.65211609526906 3.85967279916645 19.3259296159127 3.85159796225348 4.48945968415752 4.67000173221326 3.45041322250781 3.66869271660154 3.47891621806338 3.76246413410528 4.18183025799746 3.66725296957953 5.09788511459998 4.77824861111382 3.5945959669676 2.50791736346732 ___________________ Test results for ¦Ã1 Column B CNO vs. vs. Column A Saline Wilcoxon matched-pairs signed rank test P value 0.3223 Exact or approximate P value? Exact P value summary ns Significantly different (P < 0.05)? No One- or two-tailed P value? Two-tailed Sum of positive, negative ranks 17 , -38 Sum of signed ranks (W) -21 Number of pairs 10 Median of differences Median -0.06955 How effective was the pairing? rs (Spearman) 0.7212 P value (one tailed) 0.0117 P value summary * Was the pairing significantly effective? Yes __________________ ¦Ã2, switching period Saline CNO 0.206528465819321 -0.0221797637530381 0.202474149663467 -0.0399806696756066 0.342409166243662 0.298600826774075 0.438051909265542 0.453933793992928 0.783792714012463 0.607051335179124 0.349242353432389 0.459076630165137 0.391045398886974 0.092573324361141 0.414180276659953 0.298845489327744 0.547848633680939 0.315123197978425 0.558860956789496 0.226479324098977 ___________________ Test results for ¦Ã2 Column B CNO vs. vs. Column A Saline Wilcoxon matched-pairs signed rank test P value 0.0137 Exact or approximate P value? Exact P value summary * Significantly different (P < 0.05)? Yes One- or two-tailed P value? Two-tailed Sum of positive, negative ranks 4 , -51 Sum of signed ranks (W) -47 Number of pairs 10 Median of differences Median -0.2027 How effective was the pairing? rs (Spearman) 0.6364 P value (one tailed) 0.0272 P value summary * Was the pairing significantly effective? Yes ______________________________________________________________________________________________________________________________________________________________________ Decison criterion (DC) shift curve, inferenced from Model 1 parameters during stable period, for saline/CNO sessions (each column contains data from one animal) Trials Before Switch Saline - Low Bound Saline - Low Bound Saline - Low Bound Saline - Low Bound Saline - Low Bound Saline - Low Bound Saline - Low Bound Saline - Low Bound Saline - Low Bound Saline - Low Bound Saline - High Bound Saline - High Bound Saline - High Bound Saline - High Bound Saline - High Bound Saline - High Bound Saline - High Bound Saline - High Bound Saline - High Bound Saline - High Bound CNO - Low Bound CNO - Low Bound CNO - Low Bound CNO - Low Bound CNO - Low Bound CNO - Low Bound CNO - Low Bound CNO - Low Bound CNO - Low Bound CNO - Low Bound CNO - High Bound CNO - High Bound CNO - High Bound CNO - High Bound CNO - High Bound CNO - High Bound CNO - High Bound CNO - High Bound CNO - High Bound CNO - High Bound 15 -0.8473 -0.8473 -0.8473 -0.8473 -0.8473 -0.8473 -0.8473 -0.8473 -0.8473 -0.8473 0.8473 0.8473 0.8473 0.8473 0.8473 0.8473 0.8473 0.8473 0.8473 0.8473 -0.8473 -0.8473 -0.8473 -0.8473 -0.8473 -0.8473 -0.8473 -0.8473 -0.8473 -0.8473 0.8473 0.8473 0.8473 0.8473 0.8473 0.8473 0.8473 0.8473 0.8473 0.8473 14 -0.913026086777936 -0.8881599810768 -0.898968268635338 -0.873355580158489 -1.01378127606529 -0.886213175874649 -0.838674485246268 -1.05901890081536 -0.903596399204547 -0.864106569563931 0.928591808720632 0.888939492285432 0.964064801089599 0.81146852728433 1.08196934872985 0.902886298647845 0.93458936679881 0.934274261415308 0.840730557223767 0.925564438356275 -0.9499842880436 -0.978485470773072 -0.814090563210609 -0.934655804435268 -0.921312541220016 -0.893818973053974 -0.938358997079476 -0.981551979864957 -0.923304029289618 -0.909600500721919 0.904517967626547 0.976610994128362 1.04361086049571 0.919436196829739 0.956562665162721 0.905368107673732 1.00611487189171 0.94752208484711 0.79753063324288 0.808530540356217 13 -0.989259390965284 -1.0796316729369 -0.918693699696635 -0.829249971064294 -0.998128191505849 -0.911281482010502 -0.992905570263376 -1.19749952135316 -0.98413757419015 -0.863402800568438 0.986675828430397 1.08219454897543 1.01490146975461 1.01809668893872 1.25027003487391 0.970870072052793 1.09226104795597 1.17948716324163 0.999323732415338 1.01084429796561 -1.00728422899931 -1.03391333497856 -1.01068895807064 -0.876237568893774 -0.893345457734294 -0.945558007164563 -0.99753504960931 -1.00090125856161 -1.1566404312175 -0.905300547384237 1.01258035608378 0.932775430382017 1.13466147605912 1.17984555676083 0.992772236748874 0.952499826416735 1.0383730647667 1.13716407781722 1.0548930927125 0.825757602116867 12 -1.05476099440035 -1.20232524736099 -0.906480785174402 -0.830405529951296 -1.23785300371931 -0.945760708482649 -1.21222316578222 -1.41774058845168 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1.34265787602462 1.41234615381216 1.03227439916002 0.610956575376881 6 -1.43676857975281 -1.41932739868624 -1.27792171177336 -1.16589757377309 -1.6019039489964 -1.34308361685192 -1.1984033281518 -1.9124301363435 -1.15868526566818 -1.48119816820831 1.45089799401971 1.52932974872348 1.47110697577621 1.31001969690127 1.72374562570082 1.39478007392019 1.25663976902534 1.79510271607056 1.27303281457927 1.38462913896645 -1.4468270244033 -1.29380505926887 -1.2032538525205 -0.943090786221852 -1.33307620691141 -1.43677648262055 -1.39723719490343 -1.47298051416771 -1.14489401323037 -0.889884829406403 1.47617240731127 1.22721183111788 1.30301725122025 1.16175422620974 1.23454425733274 1.46782923723355 1.54112827274556 1.51530247497308 1.21844172064908 0.996326636729359 5 -1.51359201973014 -1.56359822474174 -1.36591404562119 -1.1874618672559 -1.77126162873543 -1.40660775615305 -1.31519207231415 -2.00930542990266 -1.27677205667782 -1.58549752787426 1.51891627526499 1.54217616736978 1.56216044882939 1.45724427709581 1.83224856111717 1.45809272096578 1.14589648379893 1.92597891089613 1.14908234082971 1.40051422743418 -1.51604127623973 -1.30281444480698 -1.28889899681296 -1.14636810391899 -1.3454924102512 -1.53056569600917 -1.50853213398 -1.59788255971468 -1.35407227098875 -0.963107091554902 1.52324546066621 1.21539483904934 1.38055983647672 1.12553872429723 1.24022700109843 1.56488582029514 1.60163757131464 1.66559621710686 1.19810330907907 1.19841922356215 4 -1.54823151288075 -1.5525174262165 -1.4232618226691 -1.31154788423855 -1.72530878964437 -1.46965269420101 -1.32390210147146 -1.99760864580744 -1.18021592507881 -1.58275199710951 1.5994024105082 1.6149323783844 1.6153274097222 1.22267938065887 1.79362815583652 1.54921360529483 1.41984748959135 1.96519597935686 0.975441781555658 1.39972032526015 -1.50748226124171 -1.37623587009622 -1.43667689048394 -1.09346389559208 -1.1856874928672 -1.65005552927711 -1.56444869338809 -1.61803977166427 -1.55317379520293 -1.00744646083101 1.573122175083 1.19561463471805 1.52578903470656 1.13187978657237 1.23126629819137 1.67346314735146 1.72121665634237 1.71299593585264 1.44538552553558 1.20504420000613 3 -1.6250784263646 -1.60668496463593 -1.47636804644845 -1.3625387007788 -1.60227811921191 -1.52131706327485 -1.50313868378617 -2.01135502111433 -1.13948390328518 -1.46818889305034 1.64043286173197 1.63300587164629 1.6773839499422 1.34541474796798 1.73460869006425 1.62078048086787 1.45516668243058 1.93669879238276 0.858795236009765 1.44825888320795 -1.58068955092179 -1.38051861211781 -1.51261894388852 -1.14053076679406 -1.20589289572515 -1.75740060439188 -1.73063626319001 -1.57797148873103 -1.68457483075779 -0.962823981813098 1.58126077111154 1.25830312839027 1.65009534205472 1.06003577019848 1.1395017938629 1.84137520336517 1.84223903515144 1.67005993683895 1.01388642008842 1.0513345222614 2 -1.68621768788474 -1.70448087257179 -1.51367844241482 -1.37388017049665 -1.63633762780662 -1.60227966440761 -1.43043560965659 -1.8233323469228 -1.19538161045098 -1.3500055462861 1.70714841165552 1.58511354521892 1.69485793650187 1.22484450205119 1.89462893432629 1.71947888537871 1.60863617340322 1.81925550629653 1.03128006723562 1.30053348391014 -1.65277392281476 -1.33307185881258 -1.45804498473692 -1.1501357106808 -1.31660964052725 -1.92333246909562 -1.80342458707546 -1.60110863280498 -1.50410044680445 -0.832859788911264 1.61468077546658 1.24248796792257 1.58611010475044 1.08603144986568 1.22320032838946 1.93536070458904 1.92615272144478 1.70318539667451 0.742535202779764 1.07937488914 1 -1.74786569562144 -1.66411725885872 -1.54142274529732 -1.47722501221948 -1.76000388460536 -1.67342632638265 -1.36624045772492 -1.77297454060617 -1.16045283267245 -1.36515156107973 1.76779224397822 1.57878091131318 1.71464717637012 1.24994016546701 1.91823930965724 1.78840402826984 1.45814781499459 1.8323070318314 1.20582021984925 1.4130487081344 -1.74828590334588 -1.22542828399652 -1.38183961131864 -0.984058777964173 -1.43521812324229 -2.07979668667104 -1.95900056529579 -1.667774843818 -1.12028874724571 -0.975914883698296 1.65464395089426 1.27502674886844 1.5077700730764 1.14475949244581 1.33246971468097 2.0809258188376 2.03080886178902 1.71733525703701 1.0290047841744 1.06936335899449 ___________________________________________________________ ANOVA results for Low Bound DC curve, Model 1, stable period Two-way RM ANOVA Matching: Both factors Alpha 0.05 Source of Variation % of total variation P value P value summary Significant? Trial 53.39 <0.0001 **** Yes Manip 2.135 0.1494 ns No Interaction: Trial x Manip 0.9302 0.4734 ns No Interaction: Trial x Subjects 10.26 Interaction: Manip x Subjects 7.736 Subjects 17.05 ANOVA table SS DF MS F (DFn, DFd) P value Trial 13.02 14 0.9298 F (14, 126) = 46.85 P<0.0001 Manip 0.5207 1 0.5207 F (1, 9) = 2.484 P=0.1494 Interaction: Trial x Manip 0.2268 14 0.0162 F (14, 126) = 0.984 P=0.4734 Interaction: Trial x Subjects 2.501 126 0.01985 Interaction: Manip x Subjects 1.886 9 0.2096 Subjects 4.157 9 0.4619 Residual 2.074 126 0.01646 ___________________________________________________________________ Multiple comparison tests for Low Bound DC curve, Model 1, stable period Compare each cell mean with the other cell mean in that row Number of families 1 Number of comparisons per family 15 Alpha 0.05 Sidak's multiple comparisons test Mean Diff. 95.00% CI of diff. Significant? Summary Adjusted P Value Saline - Low Bound - CNO - Low Bound Row 1 0 -0.1713 to 0.1713 No ns >0.9999 Row 2 0.01063 -0.1606 to 0.1819 No ns >0.9999 Row 3 0.006321 -0.1649 to 0.1776 No ns >0.9999 Row 4 -0.04177 -0.213 to 0.1295 No ns >0.9999 Row 5 -0.06476 -0.236 to 0.1065 No ns 0.9893 Row 6 -0.1193 -0.2906 to 0.05192 No ns 0.4544 Row 7 -0.1003 -0.2716 to 0.07095 No ns 0.7270 Row 8 -0.1195 -0.2907 to 0.05178 No ns 0.4525 Row 9 -0.1739 -0.3451 to -0.002639 Yes * 0.0436 Row 10 -0.1434 -0.3146 to 0.02787 No ns 0.1876 Row 11 -0.1441 -0.3154 to 0.02711 No ns 0.1816 Row 12 -0.1122 -0.2835 to 0.05902 No ns 0.5561 Row 13 -0.07828 -0.2495 to 0.09298 No ns 0.9441 Row 14 -0.07406 -0.2453 to 0.0972 No ns 0.9643 Row 15 -0.09513 -0.2664 to 0.07613 No ns 0.7936 Test details Mean 1 Mean 2 Mean Diff. SE of diff. N1 N2 t DF Saline - Low Bound - CNO - Low Bound Row 1 -0.8473 -0.8473 0 0.05738 10 10 0 126 Row 2 -0.9139 -0.9245 0.01063 0.05738 10 10 0.1852 126 Row 3 -0.9764 -0.9827 0.006321 0.05738 10 10 0.1102 126 Row 4 -1.074 -1.033 -0.04177 0.05738 10 10 0.7279 126 Row 5 -1.221 -1.156 -0.06476 0.05738 10 10 1.129 126 Row 6 -1.298 -1.179 -0.1193 0.05738 10 10 2.08 126 Row 7 -1.287 -1.187 -0.1003 0.05738 10 10 1.748 126 Row 8 -1.313 -1.194 -0.1195 0.05738 10 10 2.082 126 Row 9 -1.348 -1.175 -0.1739 0.05738 10 10 3.03 126 Row 10 -1.4 -1.256 -0.1434 0.05738 10 10 2.499 126 Row 11 -1.5 -1.355 -0.1441 0.05738 10 10 2.512 126 Row 12 -1.511 -1.399 -0.1122 0.05738 10 10 1.956 126 Row 13 -1.532 -1.453 -0.07828 0.05738 10 10 1.364 126 Row 14 -1.532 -1.458 -0.07406 0.05738 10 10 1.291 126 Row 15 -1.553 -1.458 -0.09513 0.05738 10 10 1.658 126 ___________________________________________________________ ANOVA results for High Bound DC curve, Model 1, stable period Two-way RM ANOVA Matching: Both factors Alpha 0.05 Source of Variation % of total variation P value P value summary Significant? Trial 45.76 <0.0001 **** Yes Manip 3.285 0.0986 ns No Interaction: Trial x Manip 0.9496 0.1738 ns No Interaction: Trial x Subjects 15.38 Interaction: Manip x Subjects 8.717 Subjects 19.7 ANOVA table SS DF MS F (DFn, DFd) P value Trial 12.39 14 0.8851 F (14, 126) = 26.77 P<0.0001 Manip 0.8897 1 0.8897 F (1, 9) = 3.392 P=0.0986 Interaction: Trial x Manip 0.2572 14 0.01837 F (14, 126) = 1.377 P=0.1738 Interaction: Trial x Subjects 4.166 126 0.03307 Interaction: Manip x Subjects 2.361 9 0.2623 Subjects 5.335 9 0.5928 Residual 1.681 126 0.01334 _________________________________________________________________________ Multiple comparison tests for High Bound DC curve, Model 1, stable period Compare each cell mean with the other cell mean in that row Number of families 1 Number of comparisons per family 15 Alpha 0.05 Sidak's multiple comparisons test Mean Diff. 95.00% CI of diff. Significant? Summary Adjusted P Value Saline - High Bound - CNO - High Bound Row 1 0 -0.1542 to 0.1542 No ns >0.9999 Row 2 -0.005273 -0.1594 to 0.1489 No ns >0.9999 Row 3 0.03436 -0.1198 to 0.1885 No ns >0.9999 Row 4 0.09386 -0.06029 to 0.248 No ns 0.6718 Row 5 0.1189 -0.03526 to 0.273 No ns 0.2946 Row 6 0.123 -0.03116 to 0.2771 No ns 0.2473 Row 7 0.1765 0.02236 to 0.3307 Yes * 0.0127 Row 8 0.1933 0.03917 to 0.3475 Yes ** 0.0041 Row 9 0.1751 0.02093 to 0.3292 Yes * 0.0139 Row 10 0.1448 -0.009398 to 0.2989 No ns 0.0846 Row 11 0.1279 -0.02628 to 0.282 No ns 0.1984 Row 12 0.07396 -0.08019 to 0.2281 No ns 0.9196 Row 13 0.1242 -0.02991 to 0.2784 No ns 0.2339 Row 14 0.1447 -0.009488 to 0.2988 No ns 0.0850 Row 15 0.1085 -0.04565 to 0.2627 No ns 0.4379 Test details Mean 1 Mean 2 Mean Diff. SE of diff. N1 N2 t DF Saline - High Bound - CNO - High Bound Row 1 0.8473 0.8473 0 0.05165 10 10 0 126 Row 2 0.9213 0.9266 -0.005273 0.05165 10 10 0.1021 126 Row 3 1.06 1.026 0.03436 0.05165 10 10 0.6652 126 Row 4 1.171 1.077 0.09386 0.05165 10 10 1.817 126 Row 5 1.282 1.163 0.1189 0.05165 10 10 2.302 126 Row 6 1.304 1.181 0.123 0.05165 10 10 2.381 126 Row 7 1.34 1.164 0.1765 0.05165 10 10 3.417 126 Row 8 1.367 1.174 0.1933 0.05165 10 10 3.743 126 Row 9 1.384 1.209 0.1751 0.05165 10 10 3.39 126 Row 10 1.459 1.314 0.1448 0.05165 10 10 2.802 126 Row 11 1.499 1.371 0.1279 0.05165 10 10 2.476 126 Row 12 1.516 1.442 0.07396 0.05165 10 10 1.432 126 Row 13 1.535 1.411 0.1242 0.05165 10 10 2.405 126 Row 14 1.559 1.414 0.1447 0.05165 10 10 2.801 126 Row 15 1.593 1.484 0.1085 0.05165 10 10 2.101 126 ______________________________________________________________________________________________________________________________________________________________________ Decison criterion (DC) shift curve, inferenced from Model 4 parameters during stable period, for saline/CNO sessions (each column contains data from one animal) Trials Before Switch Saline - Low Bound Saline - Low Bound Saline - Low Bound Saline - Low Bound Saline - Low Bound Saline - Low Bound Saline - Low Bound Saline - Low Bound Saline - Low Bound Saline - Low Bound Saline - High Bound Saline - High Bound Saline - High Bound Saline - High Bound Saline - High Bound Saline - High Bound Saline - High Bound Saline - High Bound Saline - High Bound Saline - High Bound CNO - Low Bound CNO - Low Bound CNO - Low Bound CNO - Low Bound CNO - Low Bound CNO - Low Bound CNO - Low Bound CNO - Low Bound CNO - Low Bound CNO - Low Bound CNO - High Bound CNO - High Bound CNO - High Bound CNO - High Bound CNO - High Bound CNO - High Bound CNO - High Bound CNO - High Bound CNO - High Bound CNO - High Bound 1 -0.8473 -0.8473 -0.8473 -0.8473 -0.8473 -0.8473 -0.8473 -0.8473 -0.8473 -0.8473 0.8473 0.8473 0.8473 0.8473 0.8473 0.8473 0.8473 0.8473 0.8473 0.8473 -0.8473 -0.8473 -0.8473 -0.8473 -0.8473 -0.8473 -0.8473 -0.8473 -0.8473 -0.8473 0.8473 0.8473 0.8473 0.8473 0.8473 0.8473 0.8473 0.8473 0.8473 0.8473 2 -0.895316500144871 -0.888862186220498 -0.942981953828549 -0.827427012099426 -0.996304444689788 -0.895849229572421 -0.803683518800806 -0.916074028519089 -0.924470453941931 -0.891527344620478 0.909280008265071 0.890116391579761 0.900266804378707 0.823508454981415 1.09652341626055 0.900238758799909 0.896080124629913 0.944466234854006 0.907152802264304 0.888945287031825 -0.89385649095941 -0.972294788733428 -0.844344976520941 -0.930817964405455 -0.997052061466147 -0.896583808962216 -0.910648100725329 -0.953765275911467 -0.859786456609271 -0.869991388941713 0.903901549661686 0.985657510041186 1.06014110725292 0.886859081860077 0.973781787361578 0.896409595124129 0.999527843540023 0.925017835290793 0.859786456609271 0.82132620486478 3 -0.965961948802101 -0.945483342915634 -0.969610069189041 -0.770442949438534 -1.04656627500996 -0.9268785243938 -0.93712664846588 -0.974861776391658 -0.962961141513128 -0.939526932462418 0.958182379707725 0.95426303711755 0.884981954867963 0.931512840623406 1.28869522469663 0.948951738062126 1.03491767329068 1.00585737445811 1.11866593126183 0.92960636777833 -0.950073447823024 -1.00649502251872 -1.05352407907648 -0.902665503624106 -0.993440869614476 -0.948021216465538 -0.970754283832536 -0.965904838538017 -1.17987294367472 -0.943260518646927 0.997974488408308 0.946978556209775 1.13907056814177 1.1125964952533 1.04510108161288 0.947795968718273 1.04620694959163 1.03399319986123 1.17987294367472 0.83734534888339 4 -1.02365361452369 -0.96825254305985 -0.985961675147538 -0.817371252605842 -1.25684536401686 -0.97365231602738 -1.17933415361404 -1.09889198597895 -0.886891031810938 -0.990558586908003 1.00778370567567 1.01842281097949 0.950065534142089 0.974362731481774 1.37610013645089 0.997623169707816 1.15847164113522 1.07357653765376 1.14545711025355 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1.10128731779168 1.08215491486132 1.67593842958509 1.17027883527647 1.07442215548327 1.2823773873153 0.937258082212456 1.13943641691346 -1.12770944472007 -1.06247057261854 -1.33363693159385 -1.13097201614401 -1.30973839123673 -1.18664263068519 -1.20062364315544 -1.26867296377459 -1.03376561683158 -1.01059842623593 1.23225955406702 1.0825762446434 1.32399817781513 1.08585923576523 1.24104022473941 1.18632769633015 1.25794640724805 1.39809276659996 0.818671749642033 0.913055738540545 8 -1.27715605673108 -1.19865898531039 -1.13462618683717 -0.938179765731258 -1.61531372661508 -1.21342717154461 -1.30248977699127 -1.39153426174879 -1.02806565704693 -1.21907296722649 1.24147849298491 1.32139853647834 1.16393748941696 1.06617549078933 1.70930106988075 1.22815165983512 1.00295612241795 1.39496910870965 0.993237510422072 1.20521607812644 -1.18469696051346 -1.06308035424719 -1.25038002491582 -1.13962361540147 -1.33118232493253 -1.25658848747082 -1.28182946984684 -1.27568759312752 -0.89170772494048 -1.05681666948318 1.27435875747565 1.04824563330339 1.31703144175478 0.996855371764878 1.33827285484468 1.25620251730609 1.31157950984747 1.36734092390232 1.15137537096735 0.627274471775019 9 -1.32134249297965 -1.25910616144239 -1.17874290748293 -0.977394793464155 -1.56805340252747 -1.26974864109766 -1.20745764372625 -1.4791839816712 -1.12446766128385 -1.28189236881658 1.29512892934427 1.41406210351181 1.18021808486948 1.03031205863481 1.6266533270133 1.28337488788188 1.03286255064433 1.44670824159131 1.15989232813766 1.26843759849572 -1.25805693390721 -1.17719973661587 -1.13107103704748 -0.986150010387222 -1.28805816721153 -1.32965818314072 -1.34423329364064 -1.22545979498645 -0.948959965761466 -0.974931983743579 1.33072598658935 1.12228399695029 1.1701175533926 1.12269037504385 1.24626611933724 1.3296847418244 1.39515832818757 1.3774134893045 1.05108377566846 0.631676514160937 10 -1.39209270893963 -1.33085355699438 -1.2686112648686 -1.03210903841979 -1.6276075311742 -1.32768125104892 -1.19371089958046 -1.57204462545773 -1.12491331878982 -1.35308307152485 1.36350969175456 1.44988732555449 1.25811262316676 1.05207248971123 1.66689401928877 1.3502231086748 1.22861956180104 1.53787882953556 1.32247136758943 1.33461991054919 -1.35030758214283 -1.16397492867901 -1.19330893925706 -1.03988661200048 -1.36532310951927 -1.40751873169597 -1.42823129761301 -1.3551956353734 -1.14435875844291 -0.931449655873379 1.42455950085417 1.19541209451577 1.25910766582123 1.14689726129989 1.20443895477202 1.40671064698684 1.55371118609668 1.46826653346779 1.23460766808147 0.926774655729621 11 -1.48180722937692 -1.44776702938201 -1.32733293321711 -1.02233342988216 -1.80134035357028 -1.39132296305514 -1.32014788120814 -1.63446685435408 -1.21182386898798 -1.42387847137591 1.41890430239608 1.53547002554461 1.32465682025428 1.13621759087559 1.80173783658643 1.40893833088036 1.16729695346812 1.66727709582529 1.27181710423461 1.4085977470021 -1.42295167057728 -1.1674892486145 -1.28261971439754 -1.1903390684872 -1.39629546323415 -1.49002015726726 -1.53067229968096 -1.41686520351639 -1.35765222571865 -0.958012239929186 1.51852959253921 1.17954572959243 1.26285537478108 1.10985484303359 1.29774152704464 1.48869933644432 1.64052161081099 1.55110646207591 1.24502546851415 1.1178840441015 12 -1.52093956775277 -1.50073995608909 -1.42191166735805 -1.08215302465398 -1.75038970383769 -1.44462960001297 -1.32809590168165 -1.72258697039693 -1.13613486417793 -1.50844132460382 1.50900203706253 1.62817740468824 1.38021124099344 1.06071452552122 1.77300259812236 1.49803393755711 1.42047463933875 1.76811846951419 1.06695619968091 1.4826009559072 -1.46475415761433 -1.2690185087315 -1.39453320497372 -1.14856344841228 -1.17691657011188 -1.57813593462302 -1.56149280534951 -1.42779660185718 -1.52495544578298 -1.03401372562142 1.57258615438533 1.15757497380969 1.33653301304317 1.13546022459309 1.28535544823614 1.57585370284526 1.75344787031146 1.63752920313095 1.44689118510887 1.18406854282648 13 -1.61449046459021 -1.58382465291688 -1.504055313338 -1.13572818674117 -1.61746601358217 -1.50756488088214 -1.51734642972172 -1.81196028790203 -1.13335083838362 -1.599324358527 1.57645008313352 1.71379659372519 1.4162491210167 1.12240543989461 1.7482775463779 1.56958768889196 1.41866848134872 1.81666816619684 1.00291864042225 1.56076243214854 -1.53124472395415 -1.29772721669484 -1.50041779331493 -1.18460429498271 -1.1648707644581 -1.67063278258788 -1.66053996082323 -1.43210149098869 -1.64091725190162 -1.03040372156972 1.6354493263815 1.17032408764333 1.51092328948864 1.08501096773546 1.22865813024101 1.66838873225183 1.83230868031693 1.640105300997 1.11966132798936 1.07619917264384 14 -1.69142538900282 -1.70831513080726 -1.5505823515108 -1.14452518721868 -1.64785341305169 -1.58310549284867 -1.39170062890684 -1.82762467088554 -1.21792573740855 -1.69301295437721 1.66879959686792 1.75679032346606 1.42895250221272 1.04834419385312 1.88289921209014 1.64749343276422 1.53207083476255 1.78581488847527 1.18792213115912 1.65935360468786 -1.61647633677734 -1.25482421311378 -1.45187162480965 -1.20206535051006 -1.25994085010844 -1.76864290401074 -1.71901655230598 -1.44735144405949 -1.37093230529021 -0.970602560982107 1.71112551199746 1.1414476886263 1.51542151599142 1.09867025271178 1.30923983713938 1.76690388115567 1.90348255123746 1.61523123538396 0.892850599343893 1.12029475010375 15 -1.78741301503652 -1.77262922981329 -1.51075581441575 -1.1230147102568 -1.74081270385684 -1.65000203585991 -1.2776254260782 -1.83098076034606 -1.14054763001604 -1.78772740770751 1.75847947510267 1.83177508901294 1.45456614951184 1.06822229097258 1.89851458986094 1.70753722240231 1.4059731057342 1.8994418864599 1.38479919102214 1.74764747467098 -1.69491807172287 -1.14894477957481 -1.41134119292609 -1.0710943283658 -1.37639472842643 -1.8717980621343 -1.82392990403082 -1.50089299457926 -1.02808345890102 -1.07152676928816 1.77319174752945 1.18019440574807 1.44862827944165 1.15480017293116 1.45193688042584 1.87064062594033 1.97450175406502 1.61432939280561 1.22225496986548 1.16859411319782 ___________________________________________________________ ANOVA results for Low Bound DC curve, Model 4, stable period Two-way RM ANOVA Matching: Both factors Alpha 0.05 Source of Variation % of total variation P value P value summary Significant? Trial Num 57.9 <0.0001 **** Yes Manip 0.963 0.2934 ns No Interaction: Trial Num x Manip 1.363 0.1835 ns No Interaction: Trial Num x Subjects 10.36 Interaction: Manip x Subjects 6.959 Subjects 13.42 ANOVA table SS DF MS F (DFn, DFd) P value Trial Num 11.83 14 0.8449 F (14, 126) = 50.29 P<0.0001 Manip 0.1967 1 0.1967 F (1, 9) = 1.245 P=0.2934 Interaction: Trial Num x Manip 0.2784 14 0.01989 F (14, 126) = 1.358 P=0.1835 Interaction: Trial Num x Subjects 2.117 126 0.0168 Interaction: Manip x Subjects 1.422 9 0.158 Subjects 2.742 9 0.3047 Residual 1.845 126 0.01464 ________________________________________________________________________ Multiple comparison tests for Low Bound DC curve, Model 4, stable period Compare each cell mean with the other cell mean in that row Number of families 1 Number of comparisons per family 15 Alpha 0.05 Sidak's multiple comparisons test Mean Diff. 95.00% CI of diff. Significant? Summary Adjusted P Value Saline - Low Bound - CNO - Low Bound Row 1 0 -0.1615 to 0.1615 No ns >0.9999 Row 2 0.01466 -0.1468 to 0.1762 No ns >0.9999 Row 3 0.04746 -0.1141 to 0.209 No ns 0.9993 Row 4 0.02879 -0.1327 to 0.1903 No ns >0.9999 Row 5 0.0169 -0.1446 to 0.1784 No ns >0.9999 Row 6 -0.03422 -0.1957 to 0.1273 No ns >0.9999 Row 7 -0.0371 -0.1986 to 0.1244 No ns >0.9999 Row 8 -0.05869 -0.2202 to 0.1028 No ns 0.9928 Row 9 -0.1004 -0.2619 to 0.06115 No ns 0.6410 Row 10 -0.08432 -0.2458 to 0.0772 No ns 0.8574 Row 11 -0.08493 -0.2464 to 0.07658 No ns 0.8507 Row 12 -0.08358 -0.2451 to 0.07793 No ns 0.8650 Row 13 -0.09117 -0.2527 to 0.07035 No ns 0.7746 Row 14 -0.1394 -0.3009 to 0.02208 No ns 0.1546 Row 15 -0.1623 -0.3238 to -0.0007464 Yes * 0.0480 Test details Mean 1 Mean 2 Mean Diff. SE of diff. N1 N2 t DF Saline - Low Bound - CNO - Low Bound Row 1 -0.8473 -0.8473 0 0.05412 10 10 0 126 Row 2 -0.8982 -0.9129 0.01466 0.05412 10 10 0.271 126 Row 3 -0.9439 -0.9914 0.04746 0.05412 10 10 0.8769 126 Row 4 -1.018 -1.047 0.02879 0.05412 10 10 0.5319 126 Row 5 -1.127 -1.144 0.0169 0.05412 10 10 0.3123 126 Row 6 -1.195 -1.161 -0.03422 0.05412 10 10 0.6324 126 Row 7 -1.204 -1.166 -0.0371 0.05412 10 10 0.6855 126 Row 8 -1.232 -1.173 -0.05869 0.05412 10 10 1.085 126 Row 9 -1.267 -1.166 -0.1004 0.05412 10 10 1.854 126 Row 10 -1.322 -1.238 -0.08432 0.05412 10 10 1.558 126 Row 11 -1.406 -1.321 -0.08493 0.05412 10 10 1.569 126 Row 12 -1.442 -1.358 -0.08358 0.05412 10 10 1.544 126 Row 13 -1.503 -1.411 -0.09117 0.05412 10 10 1.685 126 Row 14 -1.546 -1.406 -0.1394 0.05412 10 10 2.576 126 Row 15 -1.562 -1.4 -0.1623 0.05412 10 10 2.998 126 ___________________________________________________________ ANOVA results for High Bound DC curve, Model 4, stable period Two-way RM ANOVA Matching: Both factors Alpha 0.05 Source of Variation % of total variation P value P value summary Significant? Trial Num 53.23 <0.0001 **** Yes Manip 0.7677 0.4452 ns No Interaction: Trial Num x Manip 0.9471 0.2722 ns No Interaction: Trial Num x Subjects 12.26 Interaction: Manip x Subjects 10.84 Subjects 14.94 ANOVA table SS DF MS F (DFn, DFd) P value Trial Num 12.2 14 0.8712 F (14, 126) = 39.07 P<0.0001 Manip 0.1759 1 0.1759 F (1, 9) = 0.6375 P=0.4452 Interaction: Trial Num x Manip 0.217 14 0.0155 F (14, 126) = 1.216 P=0.2722 Interaction: Trial Num x Subjects 2.81 126 0.0223 Interaction: Manip x Subjects 2.483 9 0.2759 Subjects 3.423 9 0.3803 Residual 1.607 126 0.01275 ________________________________________________________________________ Multiple comparison tests for High Bound DC curve, Model 4, stable period Compare each cell mean with the other cell mean in that row Number of families 1 Number of comparisons per family 15 Alpha 0.05 Sidak's multiple comparisons test Mean Diff. 95.00% CI of diff. Significant? Summary Adjusted P Value Saline - High Bound - CNO - High Bound Row 1 0 -0.1507 to 0.1507 No ns >0.9999 Row 2 -0.01558 -0.1663 to 0.1351 No ns >0.9999 Row 3 -0.02313 -0.1738 to 0.1276 No ns >0.9999 Row 4 -0.002056 -0.1528 to 0.1487 No ns >0.9999 Row 5 -0.001181 -0.1519 to 0.1495 No ns >0.9999 Row 6 -0.00694 -0.1577 to 0.1438 No ns >0.9999 Row 7 0.03789 -0.1128 to 0.1886 No ns 0.9999 Row 8 0.06383 -0.08689 to 0.2145 No ns 0.9701 Row 9 0.09605 -0.05466 to 0.2468 No ns 0.6012 Row 10 0.07438 -0.07634 to 0.2251 No ns 0.9017 Row 11 0.07291 -0.0778 to 0.2236 No ns 0.9146 Row 12 0.0502 -0.1005 to 0.2009 No ns 0.9971 Row 13 0.09788 -0.05284 to 0.2486 No ns 0.5710 Row 14 0.1524 0.00166 to 0.3031 Yes * 0.0453 Row 15 0.1298 -0.02093 to 0.2805 No ns 0.1572 Test details Mean 1 Mean 2 Mean Diff. SE of diff. N1 N2 t DF Saline - High Bound - CNO - High Bound Row 1 0.8473 0.8473 0 0.0505 10 10 0 126 Row 2 0.9157 0.9312 -0.01558 0.0505 10 10 0.3086 126 Row 3 1.006 1.029 -0.02313 0.0505 10 10 0.458 126 Row 4 1.068 1.07 -0.002056 0.0505 10 10 0.04071 126 Row 5 1.147 1.148 -0.001181 0.0505 10 10 0.02339 126 Row 6 1.175 1.182 -0.00694 0.0505 10 10 0.1374 126 Row 7 1.192 1.154 0.03789 0.0505 10 10 0.7502 126 Row 8 1.233 1.169 0.06383 0.0505 10 10 1.264 126 Row 9 1.274 1.178 0.09605 0.0505 10 10 1.902 126 Row 10 1.356 1.282 0.07438 0.0505 10 10 1.473 126 Row 11 1.414 1.341 0.07291 0.0505 10 10 1.444 126 Row 12 1.459 1.409 0.0502 0.0505 10 10 0.994 126 Row 13 1.495 1.397 0.09788 0.0505 10 10 1.938 126 Row 14 1.56 1.407 0.1524 0.0505 10 10 3.017 126 Row 15 1.616 1.486 0.1298 0.0505 10 10 2.57 126 ______________________________________________________________________________________________________________________________________________________________________ Decison criterion (DC) shift curve, inferenced from Model 1 parameters during switching period, for saline/CNO sessions(each column contains data from one animal) Trials After Switch Saline - High To Low Saline - High To Low Saline - High To Low Saline - High To Low Saline - High To Low Saline - High To Low Saline - High To Low Saline - High To Low Saline - High To Low Saline - High To Low Saline - Low To High Saline - Low To High Saline - Low To High Saline - Low To High Saline - Low To High Saline - Low To High Saline - Low To High Saline - Low To High Saline - Low To High Saline - Low To High CNO - High To Low CNO - High To Low CNO - High To Low CNO - High To Low CNO - High To Low CNO - High To Low CNO - High To Low CNO - High To Low CNO - High To Low CNO - High To Low CNO - Low To High CNO - Low To High CNO - Low To High CNO - Low To High CNO - Low To High CNO - Low To High CNO - Low To High CNO - Low To High CNO - Low To High CNO - Low To High 1 1.3863 1.3863 1.3863 1.3863 1.3863 1.3863 1.3863 1.3863 1.3863 1.3863 -1.3863 -1.3863 -1.3863 -1.3863 -1.3863 -1.3863 -1.3863 -1.3863 -1.3863 -1.3863 1.3863 1.3863 1.3863 1.3863 1.3863 1.3863 1.3863 1.3863 1.3863 1.3863 -1.3863 -1.3863 -1.3863 -1.3863 -1.3863 -1.3863 -1.3863 -1.3863 -1.3863 -1.3863 2 0.995086221767007 0.710382331387723 1.22762143526406 0.625021828565682 0.86414909108375 0.845080898395662 0.966423956502148 0.998774421510348 0.820657682050418 0.407025125750671 -0.982088457425293 -0.715049983551554 -1.23356768707515 -0.661066453885767 -0.7780971328434 -0.875172535535662 -0.947424448492014 -0.994550464084952 -0.874198992391507 -0.396699555267662 0.963149309952492 1.15949907402007 1.10439999591199 1.1527884173234 0.628436490101908 0.828005001569927 0.167702136731693 1.1456258580106 1.03875165199041 0.939285923895098 -1.04701143694285 -1.16559685863162 -1.09303441583497 -0.815744237395869 -0.601619208318038 -1.28483072729922 -0.174711332240576 -1.13798260118047 -1.01368251920521 -1.00523170570271 3 0.719628218185166 0.410213056022516 1.08475033675866 0.239200787693724 0.532580252817086 0.586165770572348 0.691639315801998 0.719899008411204 0.575879458703646 0.158584316931441 -0.710620293481648 -0.376683242000891 -1.17691950286837 -0.25284424594075 -0.520962742215976 -0.534425746995168 -0.735952360582485 -0.724067408380324 -0.508263485675918 -0.149769211439415 0.765071759955056 0.958711950971753 0.906492817577032 0.742892515168316 0.294048335867458 0.639061615218464 0.124793002262501 0.992008739517575 0.857862743144854 0.606077324424217 -0.740617707234654 -0.990311225712543 -0.906385259040853 -0.363232195988816 -0.274799861777942 -1.07329253619192 -0.146862194209865 -1.04278912445767 -0.795164854805196 -0.686692067242408 4 0.336011986367791 0.0628777796388568 0.749904800400241 -0.11208456123385 -0.186589392762194 0.302848528636572 0.293734908880189 0.332804785621529 -0.138421244898082 -0.155641912514971 -0.446263957465524 -0.104505966642407 -0.801360254335404 0.068923513255081 0.0286524225312501 -0.150783645014757 -0.396909067603762 -0.303703817046763 -0.137201231294038 0.217252846456332 0.459072305919747 0.862511514548671 0.611127579870955 0.315694880981279 -0.204200492890687 0.143265012060524 -0.0191535413646415 0.468121224315214 0.483239360748855 0.430102919986159 -0.635688936922208 -0.855491655624301 -0.552334120414619 -0.0615710532050664 0.170556045411716 -0.63143399262759 -0.0107271978974011 -0.505357081232757 -0.327286145301894 -0.539288389743897 5 0.153164426025037 -0.0917377752402101 0.482356702821532 -0.298138466513629 -0.581671942191482 -0.172397208537439 0.024665045028159 0.0120486700863948 -0.431538780428728 -0.344791686982076 -0.207910122198949 0.102356181245197 -0.546808596983526 0.270925628126105 0.487270347129351 0.177530900586946 -0.235368954148706 -0.0444420915794412 0.264452841050541 0.238324162552739 0.455478119627608 0.74608190737175 0.221484458550564 0.154589752960301 -0.374502110365256 -0.00970437537975184 0.00622878400237955 0.212094450899413 0.143129666313328 0.239812823226511 -0.517750467436958 -0.776995802756842 -0.268783683711955 0.093945365062873 0.442849697394526 -0.33004352816826 -0.00901677336200667 -0.263049743961845 -0.049905705100632 -0.344397554353461 6 0.0326486826540541 -0.190852690875929 0.347398245352279 -0.366589719742546 -0.501022858291049 -0.302917440438514 -0.312014619849199 -0.147564575675771 -0.473042575993922 -0.355967519996742 -0.0154441930085841 0.0613013834865073 -0.170643288672411 0.421351596348499 0.637638852986019 0.140826840198129 0.137190742349137 0.207808975246059 0.653972175258192 0.310167522586611 0.285678217533017 0.688811427120414 0.0100194293087438 0.139164131308292 -0.434050080831061 -0.323155600055225 -0.0131313543483377 0.154075092151282 -0.0701499363133694 0.0379033675930607 -0.384392709370005 -0.662584881860367 -0.14461245589426 0.441982719150104 0.507457662661225 -0.173509603844931 0.0289506793965852 -0.052687206617483 0.181673186646137 -0.22486400655448 7 -0.0638708869253705 -0.167593194954259 0.158003628024417 -0.482634758414087 -0.713855391468443 -0.300821290387458 -0.444062125365072 -0.347304298478999 -0.802404685097592 -0.252714936259647 0.0249815601815225 0.104968986869477 -0.0193578005288543 0.466053222334993 0.690097742219844 0.160364871937373 0.332134851140891 0.338555104351575 0.785676989129815 0.530358353899633 0.116111735052803 0.55792844523834 -0.0637425850230638 -0.144753774626127 -0.670300871766498 -0.570495945960792 0.0525728669029446 0.0176832213802112 -0.279688426309738 -0.10719246004538 -0.24401004324887 -0.57509949901265 0.104483529285079 0.830361806952399 0.46821346137551 0.123349126006466 0.0156520557990723 0.0602816454949953 0.264304576087953 -0.0200654543111049 8 -0.101023547747719 -0.203106767170865 -0.0274359234091912 -0.523561952091857 -0.738495683676607 -0.46482417262266 -0.662650827584121 -0.465208419904585 -1.03552303324375 -0.189219795514979 0.137882255901045 0.191745957753309 0.137073971527481 0.473413504845629 0.741977783052392 0.259650228876326 0.430855179689783 0.480167295791023 0.690539752147286 0.286643432939859 0.0827221717042811 0.525986006166135 -0.12527787901253 -0.304570738833155 -0.572414033616915 -0.728764408628897 -0.075436886621071 -0.073432503328863 -0.301421469349142 -0.28377409183018 -0.245341849977312 -0.397956829996082 0.272551270467421 0.735983535634935 0.44315212419258 0.249696884109949 -0.00534915284050947 0.192455549554951 0.231749920836419 0.0879471079879897 9 -0.175712666023857 -0.220267759979623 -0.130637142068703 -0.561383898232855 -0.821626025910461 -0.122828923296411 -0.766549521070724 -0.574790419947805 -1.04543775108788 -0.421103517502815 0.23099601583494 0.156169426590338 0.248959003733146 0.471785135193726 0.780811199974247 0.199439218093721 0.506086336927484 0.58430538396625 0.721316189467442 0.295738984119055 0.0548474639523286 0.362897100931946 -0.263856570696228 -0.496683217972259 -0.568063168968561 -0.867408064633006 -0.0718371506590989 -0.00200787961984129 -0.454297988910058 -0.374370203215625 -0.130799545863809 -0.277895842681009 0.229813607896075 0.744864712595571 0.626417102941326 0.230636072854405 -0.00821756596694869 0.217461741867177 0.387097892149965 0.22055507623893 10 -0.208272900277041 -0.157406202296749 -0.23846983102436 -0.618244663936806 -0.696421064323895 -0.0749185688179171 -0.857428722454537 -0.682322095458527 -0.983487689535291 -0.407320637061026 0.289322963025846 0.194989836504879 0.473727223995982 0.508228068581258 0.740300755765794 0.119159162522386 0.635190050511898 0.654042553796053 0.795084682602604 0.494600472547253 0.100837181021971 0.2158988949015 -0.380401861562593 -0.508086231279874 -0.600760726313034 -0.935746269095494 -0.0938230276965637 -0.157403345455439 -0.52643069307672 -0.431561341796251 -0.120865099477361 -0.184861580467804 0.402896730871902 0.845883660672356 0.634760904682235 0.378058070506018 0.00987616466364936 0.216259206588737 0.482647710416832 0.296270737853052 11 -0.260577250914532 -0.0538268838542777 -0.320647974678892 -0.561706008508156 -0.794908399988396 -0.321641971957242 -0.886606531543474 -0.74309440321258 -0.658932629808995 -0.261240340000044 0.270267822825683 0.0920611864849488 0.572292311360254 0.548665344637884 0.662195301847353 0.224041676844576 0.654975650960527 0.690170624464603 0.73800418817393 0.311536304346391 0.00115263291038806 0.0934853046987542 -0.442231617774427 -0.676465716483183 -0.54658999134539 -1.01169820258292 -0.00507127519679026 -0.397929861755397 -0.607369436367096 -0.584570835451472 -0.0376756482486682 -0.0810551927854732 0.426028037656555 1.00973583333337 0.541169950558087 0.420622575446395 0.0274602951347196 0.159456789756659 0.586799776329805 0.474284235708799 12 -0.273291436731443 -0.0881540898722515 -0.428579900916652 -0.459238265209816 -0.563056332267263 -0.202983054427379 -0.892717003784615 -0.783210426675267 -0.564839139078237 -0.365121156993798 0.271931376177548 0.0939964456235517 0.652292560468491 0.534542890241459 0.627720090239453 0.153444461162274 0.769244818896147 0.717715096337091 0.762023502602641 0.43248941802584 -0.170455748991288 0.00838598398642141 -0.437754535068093 -0.585312625231535 -0.625449085468815 -0.936206887017382 0.00791397024581898 -0.291002377942338 -0.509360427435598 -0.694576543800631 -0.0404809114860774 -0.0081345330135921 0.360233007039673 1.18667582671715 0.581476731818469 0.443659218050285 -0.0882960224821115 0.270162736306197 0.635916686349514 0.623534981839364 13 -0.331043800583493 -0.200297000833161 -0.570496658338982 -0.433462502980311 -0.68576339058613 -0.0884067790996558 -0.885401513016597 -0.756783449878769 -0.5129051789392 -0.426266930965392 0.302562097414167 0.174610323379816 0.784974999812749 0.576633251982617 0.626351038201515 0.315029464611142 0.746128437994738 0.770725351269391 0.607450332356428 0.400519423467701 -0.210000022930185 -0.040060952807048 -0.353994653607659 -0.719341707201718 -0.551433991603704 -0.990785041258823 -0.0133903223041698 -0.3859715260345 -0.422080357789433 -0.799222186811215 0.0591691775317233 0.0551859991027215 0.362804016472196 1.30863541871091 0.516245543358307 0.506652554646595 -0.0261151075473543 0.315926497716623 0.633147004730259 0.726915236641565 14 -0.29297760056436 -0.216861031338336 -0.751244078218482 -0.44630126921021 -0.855624330292643 -0.189777205980835 -0.850483358169389 -0.744466836887155 -0.331776933786577 -0.413714042271464 0.320019829299223 0.119883172320831 0.884222435644385 0.582455390017962 1.14759175301983 0.0767254426001009 0.819283168391142 0.794552864190832 0.70447540188453 0.34821696058984 -0.241219546740605 -0.171060497087088 -0.321548989366266 -0.937989234233023 -0.447346941355796 -0.857408740898204 0.00667771359535944 -0.36602836071432 -0.398105312677552 -0.918957788221321 0.102742197164674 0.167131650399293 0.467805993608318 1.32976010254926 0.604550730711451 0.735051206108934 -0.135861112238655 0.440705110797049 0.612610616895422 0.808642985451479 15 -0.323613285418325 -0.226914143706613 -0.729371622720953 -0.446710009156439 -0.761036162785897 -0.202537524051677 -0.863041085531078 -0.773032994143296 -0.571970712421651 -0.480363821465204 0.332118674160573 0.0676013255825075 0.881242460556419 0.447664599809828 1.00402757158197 0.24030361812749 0.80621187433016 0.8086533285584 0.50565311961784 0.199465500821419 -0.19938472643715 -0.255809921009672 -0.284684134651324 -1.08278545240421 -0.544294044915264 -0.985916773122292 0.0558222421295342 -0.260456126650515 -0.495639125533154 -0.961874876342066 0.144006819643576 0.293660849524898 0.487015673835151 1.51609184794871 0.583095552533718 0.672054786917495 -0.112390518450351 0.384872990305012 0.64596435541032 0.908657174652613 ___________________________________________________________ ANOVA results for Low Bound DC curve, Model 1, switching period Two-way RM ANOVA Matching: Both factors Alpha 0.05 Source of Variation % of total variation P value P value summary Significant? Trial Loc 78.55 <0.0001 **** Yes Manip 1.177 0.1076 ns No Interaction: Trial Loc x Manip 0.6156 0.4784 ns No Interaction: Trial Loc x Subjects 3.568 Interaction: Manip x Subjects 3.318 Subjects 7.112 ANOVA table SS DF MS F (DFn, DFd) P value Trial Loc 94.55 14 6.754 F (14, 126) = 198.1 P<0.0001 Manip 1.417 1 1.417 F (1, 9) = 3.193 P=0.1076 Interaction: Trial Loc x Manip 0.741 14 0.05293 F (14, 126) = 0.9791 P=0.4784 Interaction: Trial Loc x Subjects 4.295 126 0.03409 Interaction: Manip x Subjects 3.993 9 0.4437 Subjects 8.56 9 0.9511 Residual 6.811 126 0.05406 ________________________________________________________________________ Multiple comparison tests for Low Bound DC curve, Model 1, switching period Compare each cell mean with the other cell mean in that row Number of families 1 Number of comparisons per family 15 Alpha 0.05 Sidak's multiple comparisons test Mean Diff. 95.00% CI of diff. Significant? Summary Adjusted P Value Saline - High To Low - CNO - High To Low Row 1 0 -0.3103 to 0.3103 No ns >0.9999 Row 2 -0.06674 -0.3771 to 0.2436 No ns >0.9999 Row 3 -0.1168 -0.4272 to 0.1935 No ns 0.9898 Row 4 -0.2064 -0.5167 to 0.1039 No ns 0.5314 Row 5 -0.3043 -0.6146 to 0.006043 No ns 0.0594 Row 6 -0.2745 -0.5848 to 0.03581 No ns 0.1313 Row 7 -0.2325 -0.5429 to 0.07778 No ns 0.3376 Row 8 -0.2555 -0.5658 to 0.05485 No ns 0.2074 Row 9 -0.216 -0.5263 to 0.09436 No ns 0.4566 Row 10 -0.1607 -0.471 to 0.1496 No ns 0.8646 Row 11 -0.06859 -0.3789 to 0.2417 No ns >0.9999 Row 12 -0.03874 -0.3491 to 0.2716 No ns >0.9999 Row 13 -0.04045 -0.3508 to 0.2699 No ns >0.9999 Row 14 -0.04402 -0.3543 to 0.2663 No ns >0.9999 Row 15 -0.03636 -0.3467 to 0.274 No ns >0.9999 Test details Mean 1 Mean 2 Mean Diff. SE of diff. N1 N2 t DF Saline - High To Low - CNO - High To Low Row 1 1.386 1.386 0 0.104 10 10 0 126 Row 2 0.846 0.9128 -0.06674 0.104 10 10 0.6419 126 Row 3 0.5719 0.6887 -0.1168 0.104 10 10 1.124 126 Row 4 0.1485 0.355 -0.2064 0.104 10 10 1.985 126 Row 5 -0.1248 0.1795 -0.3043 0.104 10 10 2.926 126 Row 6 -0.227 0.04752 -0.2745 0.104 10 10 2.64 126 Row 7 -0.3417 -0.1092 -0.2325 0.104 10 10 2.236 126 Row 8 -0.4411 -0.1856 -0.2555 0.104 10 10 2.457 126 Row 9 -0.484 -0.2681 -0.216 0.104 10 10 2.077 126 Row 10 -0.4924 -0.3317 -0.1607 0.104 10 10 1.545 126 Row 11 -0.4863 -0.4177 -0.06859 0.104 10 10 0.6596 126 Row 12 -0.4621 -0.4234 -0.03874 0.104 10 10 0.3725 126 Row 13 -0.4891 -0.4486 -0.04045 0.104 10 10 0.3891 126 Row 14 -0.5093 -0.4653 -0.04402 0.104 10 10 0.4234 126 Row 15 -0.5379 -0.5015 -0.03636 0.104 10 10 0.3497 126 ___________________________________________________________ ANOVA results for High Bound DC curve, Model 1, switching period Two-way RM ANOVA Matching: Both factors Alpha 0.05 Source of Variation % of total variation P value P value summary Significant? Trial 77.62 <0.0001 **** Yes Manip 1.266 0.0417 * Yes Interaction: Trial x Manip 0.4814 0.7135 ns No Interaction: Trial x Subjects 3.656 Interaction: Manip x Subjects 2.022 Subjects 9.232 ANOVA table SS DF MS F (DFn, DFd) P value Trial 95.82 14 6.844 F (14, 126) = 191.1 P<0.0001 Manip 1.562 1 1.562 F (1, 9) = 5.633 P=0.0417 Interaction: Trial x Manip 0.5943 14 0.04245 F (14, 126) = 0.7567 P=0.7135 Interaction: Trial x Subjects 4.514 126 0.03582 Interaction: Manip x Subjects 2.496 9 0.2774 Subjects 11.4 9 1.266 Residual 7.068 126 0.0561 ________________________________________________________________________ Multiple comparison tests for High Bound DC curve, Model 1, switching period Compare each cell mean with the other cell mean in that row Number of families 1 Number of comparisons per family 15 Alpha 0.05 Sidak's multiple comparisons test Mean Diff. 95.00% CI of diff. Significant? Summary Adjusted P Value Saline - Low To High - CNO - Low To High Row 1 0 -0.3161 to 0.3161 No ns >0.9999 Row 2 0.08815 -0.228 to 0.4043 No ns 0.9996 Row 3 0.133 -0.1831 to 0.4491 No ns 0.9718 Row 4 0.1923 -0.1238 to 0.5084 No ns 0.6733 Row 5 0.2529 -0.06316 to 0.5691 No ns 0.2434 Row 6 0.2867 -0.02943 to 0.6028 No ns 0.1100 Row 7 0.2386 -0.07747 to 0.5547 No ns 0.3263 Row 8 0.2265 -0.0896 to 0.5426 No ns 0.4086 Row 9 0.1956 -0.1205 to 0.5117 No ns 0.6477 Row 10 0.1944 -0.1217 to 0.5105 No ns 0.6570 Row 11 0.1237 -0.1924 to 0.4398 No ns 0.9852 Row 12 0.1051 -0.211 to 0.4212 No ns 0.9971 Row 13 0.08464 -0.2315 to 0.4008 No ns 0.9998 Row 14 0.06643 -0.2497 to 0.3825 No ns >0.9999 Row 15 -0.02301 -0.3391 to 0.2931 No ns >0.9999 Test details Mean 1 Mean 2 Mean Diff. SE of diff. N1 N2 t DF Saline - Low To High - CNO - Low To High Row 1 -1.386 -1.386 0 0.1059 10 10 0 126 Row 2 -0.8458 -0.9339 0.08815 0.1059 10 10 0.8323 126 Row 3 -0.5691 -0.702 0.133 0.1059 10 10 1.255 126 Row 4 -0.2026 -0.3949 0.1923 0.1059 10 10 1.815 126 Row 5 0.05063 -0.2023 0.2529 0.1059 10 10 2.388 126 Row 6 0.2384 -0.04826 0.2867 0.1059 10 10 2.707 126 Row 7 0.3414 0.1027 0.2386 0.1059 10 10 2.253 126 Row 8 0.383 0.1565 0.2265 0.1059 10 10 2.138 126 Row 9 0.4196 0.224 0.1956 0.1059 10 10 1.846 126 Row 10 0.4905 0.2961 0.1944 0.1059 10 10 1.835 126 Row 11 0.4764 0.3527 0.1237 0.1059 10 10 1.168 126 Row 12 0.5015 0.3965 0.1051 0.1059 10 10 0.9919 126 Row 13 0.5305 0.4459 0.08464 0.1059 10 10 0.7991 126 Row 14 0.5797 0.5133 0.06643 0.1059 10 10 0.6272 126 Row 15 0.5293 0.5523 -0.02301 0.1059 10 10 0.2172 126 ______________________________________________________________________________________________________________________________________________________________________ Decison criterion (DC) shift curve, inferenced from Model 4 parameters during switching period, for saline/CNO sessions(each column contains data from one animal) Trials After Switch Saline - High To Low Saline - High To Low Saline - High To Low Saline - High To Low Saline - High To Low Saline - High To Low Saline - High To Low Saline - High To Low Saline - High To Low Saline - High To Low Saline - Low To High Saline - Low To High Saline - Low To High Saline - Low To High Saline - Low To High Saline - Low To High Saline - Low To High Saline - Low To High Saline - Low To High Saline - Low To High CNO - High To Low CNO - High To Low CNO - High To Low CNO - High To Low CNO - High To Low CNO - High To Low CNO - High To Low CNO - High To Low CNO - High To Low CNO - High To Low CNO - Low To High CNO - Low To High CNO - Low To High CNO - Low To High CNO - Low To High CNO - Low To High CNO - Low To High CNO - Low To High CNO - Low To High CNO - Low To High 1 1.3863 1.3863 1.3863 1.3863 1.3863 1.3863 1.3863 1.3863 1.3863 1.3863 -1.3863 -1.3863 -1.3863 -1.3863 -1.3863 -1.3863 -1.3863 -1.3863 -1.3863 -1.3863 1.3863 1.3863 1.3863 1.3863 1.3863 1.3863 1.3863 1.3863 1.3863 1.3863 -1.3863 -1.3863 -1.3863 -1.3863 -1.3863 -1.3863 -1.3863 -1.3863 -1.3863 -1.3863 2 0.926654276792498 0.840952867404442 1.26376584035532 0.743315676942814 0.715413531396227 0.803107426106489 1.11420286306665 1.0267746421183 1.02035626368665 0.395282205968698 -0.926654276792498 -0.840952867404442 -1.26376584035532 -0.743315676942814 -0.715413531396227 -0.803107426106489 -1.11420286306665 -1.0267746421183 -1.02035626368665 -0.395282205968698 0.986424080504778 1.0807754046833 1.03413006453485 0.941408713277107 0.661108291300916 0.778821837371559 0.217971338996424 1.05139919843457 0.978266001513195 1.00585554482329 -0.986424080504778 -1.0807754046833 -1.03413006453485 -0.941408713277107 -0.661108291300916 -0.778821837371559 -0.217971338996424 -1.05139919843457 -0.978266001513195 -1.00585554482329 3 0.619410047390772 0.510136135898257 1.15206239576498 0.398556009225242 0.369196076538138 0.465253940609817 0.895511808458432 0.760489191154263 0.751011256470158 0.112708665047595 -0.619410047390772 -0.510136135898257 -1.15206239576498 -0.398556009225242 -0.369196076538138 -0.465253940609817 -0.895511808458432 -0.760489191154264 -0.751011256470159 -0.112708665047595 0.701891702084467 0.84258492055713 0.771423927270327 0.639291903220124 0.315273874938193 0.437541264060312 0.0342721666478351 0.797403357475911 0.690329921169021 0.729817050459316 -0.701891702084467 -0.84258492055713 -0.771423927270327 -0.639291903220124 -0.315273874938193 -0.437541264060312 -0.0342721666478351 -0.797403357475911 -0.690329921169021 -0.729817050459316 4 0.259140272941578 0.185722946854601 0.848815189722152 -0.0491306984721959 -0.257354342544434 0.160391371945476 0.489717809591945 0.397590543222083 0.239709301633537 -0.185197667462091 -0.352078082560273 -0.214175196643911 -0.857627190324011 -0.0145859428755193 0.0707370296843238 -0.146267600299313 -0.548662152806526 -0.324312494274553 -0.423860102870828 0.232586462043656 0.51117444084091 0.678055119881516 0.475920964899005 0.150421774786253 -0.183528257610289 -0.0296357702066152 -0.0238449848075298 0.443850745395566 0.357386353582558 0.408743254595152 -0.514218722140346 -0.672436921201611 -0.452501292210843 -0.0936800505371373 0.122823124092377 0.0124203965754226 0.0509602834762392 -0.437414073317737 -0.290190965778335 -0.39112819605412 5 0.0699547256205914 -0.0110715431909029 0.592513523590196 -0.289174374581567 -0.618015212541724 -0.147186638440066 0.209576692185257 0.0459705815525191 -0.136623492316383 -0.363284586853771 -0.13207780053864 0.0130006571229909 -0.674819213664268 0.231116615901248 0.59635646733092 0.14124501055331 -0.343211701936154 -0.0968346544626581 -0.0541619799709725 0.272214608248663 0.375469255052432 0.549785797290699 0.155952747630309 -0.124818466317401 -0.360695320259426 -0.10846466961251 -0.0670888482546928 0.198697091982809 0.066829127528339 0.190880859201513 -0.377722397961537 -0.548672179643902 -0.197031693739091 0.106608916644123 0.422803538394463 0.236516080416013 0.0402120271560745 -0.200252056015326 -0.047216555643424 -0.183132802315215 6 -0.0461774794750267 -0.141698950524387 0.399149627145743 -0.286467037828073 -0.69216735720768 -0.30354422127746 -0.107590186311496 -0.115056405460991 -0.296219005909057 -0.383015512576372 0.0459578079556981 0.0793478815861113 -0.336964932607607 0.402681970616277 0.718313614703692 0.225631338129543 -0.00700383997496628 0.167228766204208 0.282399143082049 0.342341436145393 0.276298619468467 0.447433755847011 -0.042918619571782 -0.311728630942281 -0.445183969226296 -0.397591529276488 -0.0690159064215788 0.0357554981922445 -0.138207484851973 0.0328067782557037 -0.282076861422326 -0.44774146878652 -0.0591548021592133 0.242621164265641 0.505155130782193 0.333720503312135 0.0546217611160929 0.00352426587696625 0.163632839034779 0.0181108322040605 7 -0.123804547588132 -0.175945474643055 0.182593609020316 -0.460236163467757 -0.805081214239537 -0.388269909683698 -0.247491530437921 -0.333725651057378 -0.570214028157156 -0.295497990957113 0.0823520178428098 0.1553259547558 -0.200177955881486 0.415027237084224 0.781250780887754 0.295061456485848 0.214333881169841 0.299089495661448 0.530117710506534 0.53881943413805 0.207038280061755 0.365287124502206 -0.15145608114425 -0.381913876020093 -0.66759089856371 -0.468207359614353 -0.044957501341832 -0.110810962402627 -0.319968349658659 -0.0969854095430515 -0.211062818309588 -0.364612430561827 0.149084939528418 0.334984372101825 0.483722391788815 0.388329842734049 0.0528625116774131 0.134164892977231 0.213946275036443 0.101215941189482 8 -0.155040509101036 -0.230465683065455 -0.0349628785016267 -0.465798482513893 -0.826028040351588 -0.481042493794351 -0.428941638358035 -0.445983216003972 -0.811014209588161 -0.239495879560846 0.168637795735022 0.225236018434381 -0.0754814914856492 0.421646605259699 0.776406652021143 0.376370853532258 0.343347689901405 0.444543275183775 0.647992847481733 0.330118513982189 0.15906066185844 0.315355491663709 -0.232421133542998 -0.372833553530872 -0.591538446165308 -0.446669039201406 -0.0606638603979235 -0.210476004296178 -0.35554767926624 -0.160961283095434 -0.163489959153347 -0.297582929127979 0.304424315933346 0.340964662310768 0.473501399612655 0.476393889200156 0.0525859005861791 0.257153142825468 0.210060400903897 0.199260940474772 9 -0.206899052814848 -0.263538557338199 -0.172864541932167 -0.512586115153749 -0.874161315258865 -0.371807481970651 -0.551774811266235 -0.578829349724779 -0.949117952697168 -0.440862552014771 0.236640829507259 0.231913905134501 0.0809942789190189 0.345550021014488 0.848553717582845 0.376808396405197 0.422598873111408 0.552274828537155 0.734752303837187 0.329438113171373 0.12622679947544 0.264669196636134 -0.352538323251983 -0.423409011668237 -0.585622595659349 -0.556989268849778 -0.0436442887218105 -0.251581905132503 -0.473338127454046 -0.267774645052073 -0.129639411760878 -0.254210553091175 0.31491339061576 0.51525093100461 0.650742543026537 0.468483596280417 0.0404676269094822 0.338476209429454 0.325489467454449 0.270399298819506 10 -0.231236801290913 -0.238606862957459 -0.318718882257551 -0.581478214273512 -0.824354032457085 -0.355091408553641 -0.673501475826276 -0.660655734134094 -1.05076623901315 -0.436183511729134 0.271770153226253 0.235964823383558 0.309241548825676 0.424216744149357 0.850804441181378 0.371085112525243 0.55961597949058 0.600207079591525 0.863062513922071 0.505726514711567 0.102863809855177 0.22280177469127 -0.442141437401515 -0.627978970514192 -0.61315397088719 -0.496546619578011 -0.0653296609959395 -0.352210674647374 -0.519385628601505 -0.345275050725665 -0.102595696390244 -0.215954653875792 0.445691145631367 0.52012181558936 0.644208819861818 0.55011647044814 0.0627118039826266 0.400153340672094 0.406943937188856 0.347179416696389 11 -0.268157900569119 -0.18973718672686 -0.431539536192048 -0.530806862423447 -0.873297427284119 -0.461821617617022 -0.679325460231906 -0.721261127315734 -0.929922045040395 -0.310658031346672 0.253946201435132 0.214601690246044 0.431711898729375 0.466396797932714 0.782915777851673 0.389597162856701 0.620859269295928 0.619778489943527 0.925276090924085 0.350096309580971 0.0862398355682185 0.19016145160427 -0.508982145919138 -0.71015663004371 -0.565578189193588 -0.585010439442403 -0.0638670248775129 -0.482292766601734 -0.584141481686695 -0.326013792056329 -0.0818740446309085 -0.188351063710234 0.490552395488478 0.693654709024474 0.5500352726851 0.538593151901999 0.0642422645807744 0.423022940471453 0.503814058794672 0.31481335525333 12 -0.282510907812692 -0.205086275681446 -0.554529761215747 -0.416027173636746 -0.711937826991077 -0.430520968602966 -0.70700900798125 -0.716447383386191 -0.880109323477765 -0.39905768747985 0.252358435043789 0.225462943017426 0.586158319460895 0.489013183441955 0.816553745156926 0.356666217731718 0.767843333325354 0.66613423932138 0.971067054626283 0.452789413784819 0.0665828669848569 0.16236287668168 -0.519029472898842 -0.765961841554788 -0.633947623047054 -0.51228888284444 -0.0538924905648799 -0.485319215868048 -0.510685298659635 -0.357334295242404 -0.0745227732950044 -0.169052136044955 0.471323014872989 0.754755715728258 0.596182843221115 0.50342707464773 0.0521797521557332 0.500136825827131 0.532781695929298 0.329076116175552 13 -0.3230842594479 -0.270640110229989 -0.727074106845645 -0.398289017474091 -0.777960493934775 -0.389105140882153 -0.775264286386771 -0.679747621716133 -0.843445742393073 -0.455311326017877 0.276689514543786 0.255872049594212 0.726953321037495 0.540962690289327 0.792660695621909 0.424899343362715 0.81265697079727 0.71639802185681 0.9403177270056 0.423243309986651 0.0578146860986779 0.150098004738942 -0.486710974793817 -0.690374600488426 -0.575494409239259 -0.502039099849213 -0.0425796096845541 -0.547383636775294 -0.439154502986876 -0.349862221037624 -0.05746275656233 -0.15622761866208 0.492108095847194 0.682764743678807 0.527132314194879 0.483670810563246 0.0586988905901157 0.534713985967598 0.513832798420309 0.352006910209404 14 -0.277920027722232 -0.299157603586193 -0.904509085115078 -0.344972862430273 -0.886679055312238 -0.430831810205414 -0.807120035123074 -0.685700059679125 -0.777328257752705 -0.440303358271363 0.293648714281879 0.250498236267555 0.855303543561683 0.568817326983476 1.06909619291757 0.333462083707132 0.921995788163699 0.737696300420636 1.01436451718781 0.385404956586553 0.0515756723438279 0.135832561486412 -0.482509256464048 -0.639044810866807 -0.486913704559607 -0.404465456839338 -0.0505454182187917 -0.558593145662676 -0.428067989030184 -0.374637962937823 -0.0482809749949832 -0.144008329277488 0.56030728410826 0.860844005701136 0.615613289405011 0.646395050026642 0.0428923971475577 0.608753312774667 0.480765989083193 0.39380914028096 15 -0.299362698540017 -0.305208230229661 -0.945410406541012 -0.360190638022208 -0.830813933356373 -0.424209452110315 -0.809720600874653 -0.723243204569753 -0.885192087948163 -0.498119728065918 0.304984871656388 0.23532814673513 0.929507827217907 0.384638109600336 0.922987669855402 0.411456789549316 0.912112692322066 0.73754103720114 0.939959501779615 0.256960991551599 0.048440983671157 0.124711058056966 -0.459468203547665 -0.77441294603176 -0.566080995101891 -0.441464015990748 -0.0566701820033978 -0.543187034799685 -0.499025402764758 -0.407713081238806 -0.0476622666520973 -0.13003973427741 0.576051922776832 0.64132373949792 0.597103572704388 0.454959364502067 0.0404071035310876 0.605137204058356 0.536212636263711 0.398975150294237 ___________________________________________________________ ANOVA results for Low Bound DC curve, Model 4, switching period Two-way RM ANOVA Matching: Both factors Alpha 0.05 Source of Variation % of total variation P value P value summary Significant? Trial Num 80.91 <0.0001 **** Yes Manipulation 1.488 0.0203 * Yes Interaction: Trial Num x Manipulation 0.5533 0.1274 ns No Interaction: Trial Num x Subjects 4.294 Interaction: Manipulation x Subjects 1.694 Subjects 7.702 ANOVA table SS DF MS F (DFn, DFd) P value Trial Num 92.41 14 6.601 F (14, 126) = 169.6 P<0.0001 Manipulation 1.699 1 1.699 F (1, 9) = 7.904 P=0.0203 Interaction: Trial Num x Manipulation 0.632 14 0.04514 F (14, 126) = 1.481 P=0.1274 Interaction: Trial Num x Subjects 4.904 126 0.03892 Interaction: Manipulation x Subjects 1.935 9 0.2149 Subjects 8.797 9 0.9775 Residual 3.841 126 0.03048 ________________________________________________________________________ Multiple comparison tests for Low Bound DC curve, Model 4, switching period Compare each cell mean with the other cell mean in that row Number of families 1 Number of comparisons per family 15 Alpha 0.05 Sidak's multiple comparisons test Mean Diff. 95.00% CI of diff. Significant? Summary Adjusted P Value Saline - High To Low - CNO - High To Low Row 1 0 -0.233 to 0.233 No ns >0.9999 Row 2 0.01137 -0.2216 to 0.2444 No ns >0.9999 Row 3 0.007451 -0.2256 to 0.2405 No ns >0.9999 Row 4 -0.06991 -0.3029 to 0.1631 No ns 0.9991 Row 5 -0.1524 -0.3854 to 0.08062 No ns 0.5595 Row 6 -0.136 -0.3691 to 0.09697 No ns 0.7313 Row 7 -0.1548 -0.3878 to 0.0782 No ns 0.5335 Row 8 -0.2162 -0.4492 to 0.01681 No ns 0.0928 Row 9 -0.2358 -0.4689 to -0.00283 Yes * 0.0449 Row 10 -0.2234 -0.4564 to 0.00959 No ns 0.0716 Row 11 -0.1847 -0.4177 to 0.04832 No ns 0.2561 Row 12 -0.1694 -0.4024 to 0.06364 No ns 0.3855 Row 13 -0.2214 -0.4544 to 0.01159 No ns 0.0770 Row 14 -0.2617 -0.4947 to -0.0287 Yes * 0.0158 Row 15 -0.2507 -0.4837 to -0.01765 Yes * 0.0249 Test details Mean 1 Mean 2 Mean Diff. SE of diff. N1 N2 t DF Saline - High To Low - CNO - High To Low Row 1 1.386 1.386 0 0.07808 10 10 0 126 Row 2 0.885 0.8736 0.01137 0.07808 10 10 0.1456 126 Row 3 0.6034 0.596 0.007451 0.07808 10 10 0.09543 126 Row 4 0.2089 0.2789 -0.06991 0.07808 10 10 0.8954 126 Row 5 -0.06473 0.08765 -0.1524 0.07808 10 10 1.952 126 Row 6 -0.1973 -0.06124 -0.136 0.07808 10 10 1.742 126 Row 7 -0.3218 -0.167 -0.1548 0.07808 10 10 1.983 126 Row 8 -0.4119 -0.1957 -0.2162 0.07808 10 10 2.769 126 Row 9 -0.4922 -0.2564 -0.2358 0.07808 10 10 3.021 126 Row 10 -0.5371 -0.3136 -0.2234 0.07808 10 10 2.862 126 Row 11 -0.5397 -0.355 -0.1847 0.07808 10 10 2.365 126 Row 12 -0.5303 -0.361 -0.1694 0.07808 10 10 2.169 126 Row 13 -0.564 -0.3426 -0.2214 0.07808 10 10 2.836 126 Row 14 -0.5855 -0.3237 -0.2617 0.07808 10 10 3.352 126 Row 15 -0.6081 -0.3575 -0.2507 0.07808 10 10 3.21 126 ___________________________________________________________ ANOVA results for High Bound DC curve, Model 4, switching period Two-way RM ANOVA Matching: Both factors Alpha 0.05 Source of Variation % of total variation P value P value summary Significant? Trial Num 80.96 <0.0001 **** Yes Manip 1.197 0.0356 * Yes Interaction: Trial Num x Manip 0.5684 0.1365 ns No Interaction: Trial Num x Subjects 4.303 Interaction: Manip x Subjects 1.768 Subjects 7.698 ANOVA table SS DF MS F (DFn, DFd) P value Trial Num 92.54 14 6.61 F (14, 126) = 169.3 P<0.0001 Manip 1.369 1 1.369 F (1, 9) = 6.095 P=0.0356 Interaction: Trial Num x Manip 0.6497 14 0.04641 F (14, 126) = 1.458 P=0.1365 Interaction: Trial Num x Subjects 4.918 126 0.03903 Interaction: Manip x Subjects 2.021 9 0.2246 Subjects 8.799 9 0.9777 Residual 4.01 126 0.03182 ________________________________________________________________________ Multiple comparison tests for High Bound DC curve, Model 4, switching period Compare each cell mean with the other cell mean in that row Number of families 1 Number of comparisons per family 15 Alpha 0.05 Sidak's multiple comparisons test Mean Diff. 95.00% CI of diff. Significant? Summary Adjusted P Value Saline - Low To High - CNO - Low To High Row 1 0 -0.2381 to 0.2381 No ns >0.9999 Row 2 -0.01137 -0.2495 to 0.2267 No ns >0.9999 Row 3 -0.007451 -0.2455 to 0.2306 No ns >0.9999 Row 4 0.008712 -0.2294 to 0.2468 No ns >0.9999 Row 5 0.07007 -0.168 to 0.3082 No ns 0.9993 Row 6 0.1388 -0.09934 to 0.3768 No ns 0.7338 Row 7 0.1829 -0.05524 to 0.421 No ns 0.3007 Row 8 0.1806 -0.05754 to 0.4186 No ns 0.3195 Row 9 0.1619 -0.07618 to 0.4 No ns 0.4949 Row 10 0.1933 -0.04478 to 0.4314 No ns 0.2246 Row 11 0.1747 -0.06343 to 0.4128 No ns 0.3708 Row 12 0.2088 -0.02932 to 0.4469 No ns 0.1394 Row 13 0.2479 0.009847 to 0.486 Yes * 0.0344 Row 14 0.2413 0.003225 to 0.4794 Yes * 0.0443 Row 15 0.2363 -0.001793 to 0.4744 No ns 0.0534 Test details Mean 1 Mean 2 Mean Diff. SE of diff. N1 N2 t DF Saline - Low To High - CNO - Low To High Row 1 -1.386 -1.386 0 0.07978 10 10 0 126 Row 2 -0.885 -0.8736 -0.01137 0.07978 10 10 0.1425 126 Row 3 -0.6034 -0.596 -0.007451 0.07978 10 10 0.09339 126 Row 4 -0.2578 -0.2665 0.008712 0.07978 10 10 0.1092 126 Row 5 -0.004717 -0.07479 0.07007 0.07978 10 10 0.8783 126 Row 6 0.192 0.05324 0.1388 0.07978 10 10 1.739 126 Row 7 0.3111 0.1283 0.1829 0.07978 10 10 2.292 126 Row 8 0.3659 0.1853 0.1806 0.07978 10 10 2.263 126 Row 9 0.416 0.254 0.1619 0.07978 10 10 2.03 126 Row 10 0.4992 0.3059 0.1933 0.07978 10 10 2.423 126 Row 11 0.5055 0.3309 0.1747 0.07978 10 10 2.189 126 Row 12 0.5584 0.3496 0.2088 0.07978 10 10 2.617 126 Row 13 0.5911 0.3431 0.2479 0.07978 10 10 3.108 126 Row 14 0.643 0.4017 0.2413 0.07978 10 10 3.025 126 Row 15 0.6035 0.3672 0.2363 0.07978 10 10 2.962 126 ______________________________________________________________________________________________________________ Mean percent correct for non-reversing stimulus, during stable period (each row contains data from one animal) Saline CNO 83.1194 88.95337 89.04904 88.56227 95.43651 92.72051 98.95833 89.58333 96.77258 94.79458 95.89286 87.45238 91.75099 95.25132 96.93878 96.48243 95.62771 94.23701 93.66239 91.67063 ____________ Test results Column B CNO vs. vs. Column A Saline Wilcoxon matched-pairs signed rank test P value 0.2324 Exact or approximate P value? Exact P value summary ns Significantly different (P < 0.05)? No One- or two-tailed P value? Two-tailed Sum of positive, negative ranks 15 , -40 Sum of signed ranks (W) -25 Number of pairs 10 Median of differences Median -1.684 How effective was the pairing? rs (Spearman) 0.297 P value (one tailed) 0.2035 P value summary ns Was the pairing significantly effective? No ______________________________________________________________________________________________________________ Mean percent correct for non-reversing stimulus, during switching period (each row contains data from one animal) Saline CNO 92.02615 96.0084 92.6101 91.24595 96.77325 97.55485 100 97.02385 97.6911 98.497 95.68455 96.32935 95.80685 97.6601 97.9496 97.2262 98.90155 99.03275 96.37285 91.58665 ____________ Test results Column B CNO vs. vs. Column A Saline Wilcoxon matched-pairs signed rank test P value >0.9999 Exact or approximate P value? Exact P value summary ns Significantly different (P < 0.05)? No One- or two-tailed P value? Two-tailed Sum of positive, negative ranks 28 , -27 Sum of signed ranks (W) 1 Number of pairs 10 Median of differences Median 0.388 How effective was the pairing? rs (Spearman) 0.6 P value (one tailed) 0.0367 P value summary * Was the pairing significantly effective? Yes __________________________________________________________ Average block length (each row contains data from one animal) Saline CNO 75.6316 75.74475 66.2905 68.3128 68.375 68.3736 60.4028 62.75 65.52575 65.55515 70.3182 68.62855 67.49355 69.53125 62 62.7495 66.8333 65.10715 67.96665 74.93335 ____________ Test results Column B CNO vs. vs. Column A Saline Wilcoxon matched-pairs signed rank test P value 0.1309 Exact or approximate P value? Exact P value summary ns Significantly different (P < 0.05)? No One- or two-tailed P value? Two-tailed Sum of positive, negative ranks 43 , -12 Sum of signed ranks (W) 31 Number of pairs 10 Median of differences Median 0.4313 How effective was the pairing? rs (Spearman) 0.8545 P value (one tailed) 0.0014 P value summary ** Was the pairing significantly effective? Yes __________________________________________________________ Average number of switches per session (each row contains data from one animal) Saline CNO 4.4444 3.5556 3.8889 3.8889 3.6667 3.5556 3.5 2.6667 4.6667 4.5556 3.6667 3.4444 3.6667 4.6667 5.6667 5.6667 3.4444 3.7778 4.1111 3.6667 ____________ Test results Column B CNO vs. vs. Column A Saline Wilcoxon matched-pairs signed rank test P value 0.3438 Exact or approximate P value? Exact P value summary ns Significantly different (P < 0.05)? No One- or two-tailed P value? Two-tailed Sum of positive, negative ranks 16 , -36 Sum of signed ranks (W) -20 Number of pairs 10 Median of differences Median -0.1111 How effective was the pairing? rs (Spearman) 0.4985 P value (one tailed) 0.0727 P value summary ns Was the pairing significantly effective? No ________________________________________________________________________________________________________________________________________ Decision boundary distance, estimated from psychometric curves after switching period(in cyc/deg, each row contains data from one animal) Saline CNO 0.0152561659335212 0.00480270406786602 0.0122864380271328 0.0144810477129351 0.0104172622258709 0.0188157671174219 0.0269788848747637 0.0220830452105801 0.0287632749262437 0.021902783903205 0.0206154167155179 0.00803301578424083 0.0170995534692276 0.0247690205954461 0.0229898738781784 0.0282801474642712 0.0365519941787034 0.0371717216515502 0.0332701934791599 0.0318567117386557 ____________ Test results Column B CNO vs. vs. Column A Saline Wilcoxon matched-pairs signed rank test P value 0.7695 Exact or approximate P value? Exact P value summary ns Significantly different (P < 0.05)? No One- or two-tailed P value? Two-tailed Sum of positive, negative ranks 24 , -31 Sum of signed ranks (W) -7 Number of pairs 10 Median of differences Median -0.0003969 How effective was the pairing? rs (Spearman) 0.7212 P value (one tailed) 0.0117 P value summary * Was the pairing significantly effective? Yes ______________________________________________________________________________________________________________________________ Right choice bias difference between different sensory history, during stable period (each row contains data from one animal) Saline CNO 2.589044 -0.20819 6.165874 3.721772 5.143908 3.69619 2.167832 8.300248 7.358527 1.502153 11.66679 -3.29531 13.00447 9.034581 2.847986 6.233397 17.08237 25.23871 7.441509 26.17317 _____________ Test results Column B CNO vs. vs. Column A Saline Wilcoxon matched-pairs signed rank test P value 0.9219 Exact or approximate P value? Exact P value summary ns Significantly different (P < 0.05)? No One- or two-tailed P value? Two-tailed Sum of positive, negative ranks 29 , -26 Sum of signed ranks (W) 3 Number of pairs 10 Median of differences Median -1.946 How effective was the pairing? rs (Spearman) 0.3091 P value (one tailed) 0.1935 P value summary ns Was the pairing significantly effective? No ______________________________________________________________________________________________________________________________ Right choice bias difference between different sensory history, during switching period (each row contains data from one animal) Saline CNO 10.92642 6.076939 18.25729 4.753368 22.95052 16.1304 21.8222 0.49047 43.15637 28.82578 26.39801 23.69608 26.31782 11.67098 15.77001 31.34279 40.98427 30.10721 28.61856 23.61031 _____________ Test results Column B CNO vs. vs. Column A Saline Wilcoxon matched-pairs signed rank test P value 0.0645 Exact or approximate P value? Exact P value summary ns Significantly different (P < 0.05)? No One- or two-tailed P value? Two-tailed Sum of positive, negative ranks 9 , -46 Sum of signed ranks (W) -37 Number of pairs 10 Median of differences Median -8.849 How effective was the pairing? rs (Spearman) 0.4545 P value (one tailed) 0.0956 P value summary ns Was the pairing significantly effective? No