
smFRET traces are provided as one text file per molecule.
The tab-delimited text contains 3 columns as specified: 
donor intensity after donor excitation, acceptor intensity after donor excitation, acceptor intensity after acceptor excitation.

The traces contain the non-bleached part only.
They were corrected for background, leakage, direct excitation, gamma, and beta factor, as described in: Hellenkamp, Schmid, et al. Nat Meth (2018).


Schmid, Hugel, 2020