Soon after the initial discovery that diminished insulin signaling can extend lifespan, the aging field began exploring where decreased insulin signaling was required to promote longevity. By constructing animals with mosaic expression of daf-2, several cell lineages were identified that require daf-2 mutations to reproduce the entirety of the daf-2 lifespan benefit (Apfeld and Kenyon, 1998). This study was the first to clearly define a role for cell non-autonomous activity in aging and validate the significance of inter-tissue signaling during the lifespan of an organism. From here they sought to understand the effects of insulin signaling across tissues and the emergent role of the nervous system in influencing longevity came to the forefront of the field (Figure 1).

The identification of this role began through epistasis experiments. It was discovered that daf-2 mutants completely require the class O of forkhead box transcription factors (FOXO) ortholog daf-16 to extend lifespan (Gottlieb and Ruvkun, 1994), suggesting that daf-16 nuclear localization and transcriptional activity is responsible for the longevity benefits. While most of daf-16’s pro-longevity effects are thought to be tied to cell-autonomous transcription of target genes in the intestine (Libina et al., 2003), more recent studies show that a subset of target genes, dod-8, dod-11, and hsp12.6, influence muscle aging cell non-autonomously (McCormick et al., 2012). Intestinal expression of daf-16 leads to elevated induction of target genes in the nervous system and hypodermis. Conversely to daf-16, tissue-specific expression of daf-2 or phosphatidylinositol 3-kinase homolog age-1 in neurons is sufficient to partially block lifespan extension in mutant animals, whereas daf-2 or age-1 rescue in muscle is sufficient to restore metabolic function (Wolkow et al., 2000). These studies provide evidence that metabolism and aging are separable, yet interrelated, and that bi-directional signaling from metabolically active tissues to the nervous system occurs. We will expand on this understudied phenomenon in the Emerging concepts section.

Nematodes have amphid neurons with ciliated projections that relay information about their current environment to the worm. Interestingly, when these projections are genetically knocked out or laser ablated, the animals live longer, often with the requirement of daf-16 activity (Apfeld and Kenyon, 1999; Alcedo and Kenyon, 2004). These data are supported by a recent study analyzing RNA in isolated neurons of daf-16 and daf-16; daf-2 mutants that finds daf-16 target genes exclusively expressed in mechanosensory neurons (Kaletsky et al., 2016). This led to identification of a novel gene, fkh-9/FOXL1, that, when knocked out, entirely abrogates daf-2 lifespan. Another transcriptional microarray analysis identified a set of neuronally expressed genes upregulated in animals on daf-2 RNAi and downregulated in animals on daf-16 RNAi (class I) or vice versa (class II) (Silva et al., 2013). The class II hit, ins-7, predicted to encode for an insulin-like peptide, is required for daf-2 RNAi-mediated lifespan extension. ins-7 may act as a signaling molecule in insulin-mediated cell non-autonomous signaling from the nervous system to the intestine. These data are compelling as C. elegans are predicted to have 40 insulin-like peptides that both agonize and antagonize daf-2, some of which modify the lifespan of wild-type animals when neuronally overexpressed (De Rosa et al., 2019).

Foundational work from the Kenyon lab established that signaling from germ cells regulates insulin signaling by decreasing daf-16 activity throughout the organism (Hsin and Kenyon, 1999). This means that ablating the germline genetically or physically leads to daf-16 activation and increased longevity. The absence of germline signals activates a daf-12-dependent sterol signaling pathway in somatic reproductive tissues (Yamawaki et al., 2010). This pathway induces the expression of lips-17/fard-1 and produces an unknown lipophilic signal to increase sod-3/dod-8 in other somatic tissues (McCormick et al., 2012). This signaling pathway is an illustration of cells/tissues signaling to other tissues that ‘times are good/bad’.

Despite the groundbreaking discovery of microRNAs (miRNAs) in C. elegans (Reinhart et al., 2000), their size, abundance across the genome, and evolutionary conservation, little work has investigated their potential role in cell non-autonomous modulation of aging. An intriguing study begins this exploration, interrogating how mir-71 modulates lifespan (Boulias and Horvitz, 2012). mir-71 is necessary for lifespan extension in glp-1 germline mutants, while mir-71 overexpression in germline mutants extends lifespan beyond that of germline mutations alone. Rescuing mir-71 in neurons alone is sufficient to rescue the germline mutant longevity phenotype, and intestinal daf-16 expression is necessary for mir-71 overexpression to extend lifespan in germline mutants. While this study presents an incomplete picture, it provides sound evidence for future studies to consider miRNAs as a target for inter-tissue signaling during the aging process.

The absence of gonadal stem cell signaling leads to activation of intestinal transcription factors like skn-1/Nrf2 that help to globally remodel the organism’s metabolism (Steinbaugh et al., 2015). A lack of gonadal stem cells also increases circulating fatty acids (FAs) that are normally deposited in this tissue. This increase in circulating lipids may induce skn-1/Nrf2 nuclear localization that, in turn, enhances lipid metabolism and increases lifespan. nhr-49/HNF4α, a nuclear hormone receptor widely expressed throughout somatic tissues, also regulates both glp-1-mediated longevity and lipid metabolism (Ratnappan et al., 2014). Furthermore, nhr-49 overexpression extends lifespan in a daf-16- and glp-1-dependent manner. While this work cannot be directly translated to human health, these data provide initial evidence for critical signaling events between stem cells and nearby somatic tissues that influence longevity.

Multiple nutrient-sensing pathways that interact with insulin signaling are conserved from worms to mammals including the target of rapamycin (TOR) and AMP-activated protein kinase (AMPK) pathways. The latter nematode ortholog, aak-2, has increased activity under low energy conditions and is sufficient to promote longevity when overexpressed (Apfeld et al., 2004). Follow-up studies show that aak-2 activity suppresses cAMP-response element binding protein (CREB)/CREB-regulated transcription coactivator 1 (CRTC-1) transcriptional regulation and this ultimately extends lifespan (Mair et al., 2011). crtc-1 neuron-specific knockout extends lifespan without the undesirable pleiotropic effects that accompany AMPK activation, like decreased growth and fecundity (Burkewitz et al., 2015). Interestingly, nhr-49/HNF4α is activated by AAK-2 (Moreno-Arriola et al., 2016), and its neuronal expression is required for the lifespan extension in AAK-2 overexpressing animals. Furthermore, neuronal CRTC-1 is sufficient to block AMPK-mediated longevity. Neuronal crtc-1 levels modify intestinal AAK-2 activity through the neurotransmitter octopamine as a mechanism for the nervous system to convey ‘times are good’. Despite these results, many questions remain involving this signaling pathway. Octopamine is thought to be exclusively synthesized in the RIC head neurons (Alkema et al., 2005) and the two known octopamine receptors, ser-3 and octr-1, are expressed throughout inner neurons but not in peripheral tissues (Suo et al., 2006; Mills et al., 2012). This suggests that octopamine acts as an initiating signal instead of a direct signal from neurons to downstream tissues. Recent work highlights this point by showing GABAergic signaling and neuron excitability inversely changes throughout the life of short- and long-lived animals (Wu et al., 2018).

TOR and AMPK play antagonistic roles in modulating lifespan and metabolism. Downregulation of TORC1 complex proteins, like raga-1, increases lifespan (Schreiber et al., 2010) but requires neuronal aak-2 activity (Zhang et al., 2019). Moreover, neuronal TORC1 expression is sufficient to shorten lifespan, consistent with a central ‘times are good’ signal modulating longevity. RNA-seq comparisons of wild-type to raga-1 knockout and neuronal overexpression identified unc-64/syntaxin as epistatic to neuronal raga-1 activity. This work supports neural signaling as responsible for metabolic rearrangements, like increased mitochondrial fragmentation, that occur in peripheral tissues.

Although many of these studies identify the existence of neurosignaling pathways that drive metabolic changes in peripheral tissues, few have identified the signal(s) through which the nervous system transmits information to these tissues. Probing the precise mechanisms of nervous system to intestinal signaling will be an essential next step in furthering our understanding of how cell non-autonomous signaling influences aging.

A significant body of literature links increased stress resistance with longevity, leading to the hypothesis that acute moderate stress can trigger hormetic effects that extend lifespan (Calabrese et al., 2015). Eukaryotic cells have evolved several organelle-specific stress responses that, when induced, extend nematode lifespan.

Knock-down of complex IV of the electron transport chain (ETC) with cco-1 RNAi leads to delayed growth, slowed movement, reduced body length, and increased lifespan. Importantly, cco-1 knock-down exclusively in neurons increases lifespan without pleiotropic phenotypes by altering mitochondrial homeostasis in peripheral tissues (Durieux et al., 2011). Interestingly, this lifespan extension requires the mitochondrial unfolded protein response (mt-UPR), but not daf-16/insulin signaling. A follow-up study using the heat-shock protein-6 (hsp-6)p::GFP transcriptional reporter identified vps-35 as lacking peripheral mt-UPR (Zhang et al., 2018a). VPS-35 is a highly conserved protein in the retromer complex, involved in recycling Wnt and the Wnt secretion factor MG-4 (Prasad and Clark, 2006). In agreement with this, the Wnt receptor egl-20 is necessary and sufficient for cell non-autonomous mt-UPR induction and longevity. Interestingly, neuronal serotonin production is necessary for cell non-autonomous mt-UPR even though the loss of each of the four known serotonin receptors (ser-1, ser-4, ser-7, and mod-1) has no effect. These results clearly define that mitochondrial stress, through reduction of ETC activity or separate activation of the mt-UPR, can be transmitted by neurons cell non-autonomously to modify aging. As with many of these neuronal-based networks, however, the central signaling pathways remain largely uncharacterized.

Multiple labs find that neuronal activation of the endoplasmic reticulum unfolded protein response (ER-UPR) is sufficient to enhance stress resistance and extend lifespan (Taylor and Dillin, 2013; Imanikia et al., 2019b; Frakes et al., 2020; Daniele et al., 2020; Imanikia et al., 2019a). This crucial discovery stems from identifying that the constitutively active spliceoform of X-box binding protein 1 (xbp-1s), a transcription factor activated by the ER-UPR, rescues older animal survival on paraquat (Taylor and Dillin, 2013). Expression of xbp-1s in the nervous system or intestine extends lifespan, and exclusive xbp-1s expression in the neurons is sufficient to increase paraquat stress resistance in young and older animals. It is likely that neuronal ER-UPR releases an activation signal conferring xbp-1s upregulation in the intestines that rescues motility in models of proteotoxicity like Aβ, polyglutamine aggregates (Q40), and dynamin (Imanikia et al., 2019b).

More recent work begins to parse out where xbp-1s is required in the nervous system to extend lifespan. Surprisingly, expression of xbp-1s in glia, helper, and insulator cells for neurons, extends lifespan and activates the ER-UPR in peripheral tissues (Frakes et al., 2020). This xbp-1s-mediated signal requires neuropeptide signaling but not neurotransmission for intestinal induction of hsp-4, suggesting a neuropeptide is the intermediate signal from the glia cells to the intestine (Frakes et al., 2020). While it is useful to rule-out neurotransmitters as the causative signaling molecules, there remain hundreds of potential coding regions annotated as ‘neuropeptides’ in C. elegans (Li et al., 1999a; Li et al., 1999b).

Another open question surrounds whether other transcription factors that respond to the ER-UPR can recapitulate the effects of xbp-1 activation. Preliminary findings point to xbp-1 activity, not pek-1 or atf-6, two transcription factors also activated by the ER-UPR (Frakes et al., 2020), as key to modifying lifespan. This implies that uncovering the pro-longevity targets of XBP-1 will be of high interest to understand and translate these results. Neuronal activation of xbp-1s results in changes in fat metabolism, a build-up in oleic acid (OA), and leaner animals (Imanikia et al., 2019a). It is possible that the pro-longevity phenotypes associated with xbp-1s are due to increased expression of lysosomal genes, like asp-13 and vha-18, that enhance acidity and, therefore, protease activity. Furthermore, the lifespan phenotype and expansion of the ER in xbp-1s animals requires lipid depletion (Daniele et al., 2020). Initial results show enhanced intestinal lipid depletion through ehbp-1 overexpression modestly extends lifespan and partially recapitulates the effects of xbp-1s. Taken together, these data emphasize the significant role metabolically active tissues, like the intestine, play in properly responding to cues from the nervous system (summarized in Figure 2). While the ER-UPR field is beginning to narrow in on specific neuronal cells and signaling molecules, much is left to delineate in the neuronal circuitry. Understanding how, when, and where neuromodulators affect normal and pro-longevity conditions is paramount in translating these results to higher organisms and discovering therapeutic treatments to mimic these phenomena.

An organism's ability to respond to changes in the environment, such as temperature, oxygen levels, and smells, is vital to their survival. In this section, we chronicle research findings linking perception and organismal aging (Figure 3).

As poikilotherms, C. elegans are responsive to heat- and cold-shock. Generally, longevity varies inversely with temperature, where animals housed at lower temperatures (15℃) live longer than those at higher temperatures (25℃). Based on our understanding of thermodynamics, this observation seems intuitive, but several papers have challenged this theory and shown changes in lifespan across temperature are genetically modulated. In fact, many pro-longevity genetic mutations have a distinct relationship with temperature, promoting longevity at either cold or warm temperatures but rarely both (Miller et al., 2017). These data are consistent with many genes playing an active role in the physiological response to changes in temperature. Whether by modifying the perception or response to temperature, this interplay suggests that genes actively influence lifespan at various temperatures, refuting thermodynamics as the sole influence on temperature-mediated lifespan changes.

A robust body of research surrounds activating the heat-shock response (HSR) and longevity. In C. elegans, post-reproductive adulthood is accompanied by an abrupt decline in protein quality control (Walther et al., 2015; Labbadia and Morimoto, 2015; Santra et al., 2019). Thus, maintaining the ability to effectively respond to heat stress remains a hallmark of long-lived animals. It is important to note that heat shock, described in the literature as >30℃, likely uses partially distinct genetic mechanisms from warmer conditions (22℃−25℃) that will be addressed separately.

Early studies implicate the nervous system in regulating proteostasis through the HSR. Key signaling components of amphid neurons, gcy-3 and ttx-3, are necessary for proper induction of global HSR during heat shock (Prahlad et al., 2008). These early studies also implicate an unknown neurotransmitter in signaling from the nervous system to promote survival during heat shock. Optogenetic stimulation of the AFD thermosensory neurons triggers serotonin release from the ADF neurons that activates heat shock factor 1 (HSF-1) in the germline in the absence of heat shock (Tatum et al., 2015). In the absence of ser-1, a 5-HT2B serotonin receptor ortholog, HSF-1 is not re-localized to the nucleus upon AFD optogenetic excitation or heat shock. This result suggests that ser-1 is necessary for proper signaling during HSR. It is worth noting there is no predicted synapse or gap junction between AFD and ADF neurons, meaning there is likely an intermediate signaling molecule(s) and/or cells yet to be discovered.

While HSR proteins are necessary for animals to survive warmer temperatures (Lee and Kenyon, 2009), it is significant that overexpression of hsf-1 in neurons is sufficient to increase stress resistance and longevity (Douglas et al., 2015). Linking back to earlier work, intact signaling from the AFD/AIY neurons is required for heat stress resistance in worms overexpressing hsf-1 (Prahlad et al., 2008). This may be through pat-4/ integrin linked kinase (ILK) activity in the AFD/AIY (Kumsta et al., 2014). Interestingly, intestinal daf-16 activity is required for the lifespan phenotype but unnecessary for heat stress resistance (Douglas et al., 2015), suggesting these phenotypes have distinct signaling events despite their positive correlation.

Less is known about the mechanisms of cold- and warm-sensory signaling and metabolic remodeling that promotes longevity. As with the HSR, the AFD neurons are also thought to play a role in appropriately responding to warmer temperatures as laser ablation and genetic disruption exclusively shortens lifespan at 25°C (Lee and Kenyon, 2009). Mutants lacking functional CNG calcium channels, a tax-2/tax-4 heterodimer, are also short-lived at 25°C and thought to be necessary for AFD neuronal activity when exposed to warm temperatures. These results are corroborated in a recent study showing the ASJ sensory neurons also require functional tax-2/tax-4 channels to sense warm temperatures and activate intestinal daf-16 to extend lifespan (Zhang et al., 2018a; Artan et al., 2016). Experimental evidence suggests once these thermosensory neurons are activated they deploy daf-9, a cytochrome P450 ortholog, which inhibits daf-12, a nuclear hormone receptor, allowing worms to live longer at 25°C (Lee and Kenyon, 2009). An important distinction remains between AFD activation during warm- and heat-shock as many heat shock proteins, like hsp-60 and hsp-70, are not upregulated at 25°C (Lee and Kenyon, 2009). These data suggest that perception and response to temperature through thermosensory neurons are sufficient to modulate aging across temperatures. They also refute thermodynamics as the sole mechanism for how poikilotherms live shorter at higher temperatures.

In agreement with neural modulation of aging in warmer temperatures, a transient receptor potential (TRP) channel, TRPA-1, detects cold temperatures in chemosensory neurons. TRPA-1 signals through a protein kinase C (PKC) ortholog, PKC-2, to increase intestinal DAF-16 activity and therefore lifespan (Xiao et al., 2013). Loss of trpa-1 channels in the nervous system prevents the lifespan increase observed in wild-type worms at cooler temperatures (15–20°C), but does not change longevity at warmer temperatures (25°C). Interestingly, human transgenic TRPA-1 recapitulates many of these findings, suggesting a conservation in function. An unbiased screen of all sensory neurons showed trpa-1 expression in the head neuron IL1 is necessary and sufficient to rescue trpa-1 knockout. Further, genetically knocking out glutamate secretion and uptake prevents IL1 from modifying lifespan (Zhang et al., 2018b). MGL-1, the glutamate receptor implicated in this study, is only expressed in neurons, indicating there must be another neuron involved in this pathway. Knocking out serotonin signaling blocks the effects of IL1 on lifespan, and transgenic serotonin expression in the NSM enteric neuron rescues the phenotype. The intestinal GPCR ser-7 is the likely downstream receptor responding to serotonin release. This study offers a more complete model than is often presented in the field, and suggests it is feasible for other cell non-autonomous signaling pathways to be more explicitly characterized in future studies.

The nervous system in C. elegans plays a crucial role in determining nutrient quality and safety as they forage for food, and interestingly, the lack of any perceived signal (i.e. dietary restriction (DR)), can also act as a signal on its own. DR, first reported to increase lifespan in rats in 1935 (McCay et al., 1935), is an intervention that significantly limits food intake without malnutrition, and has been the most consistent intervention to increase longevity across species (Fontana and Partridge, 2015). However, since implementation of any dietary intervention for humans at a whole population level is challenging, mapping out the molecular and signaling mechanisms downstream of food perception, where they can be targeted directly, circumvents the challenges of adopting population level-DR protocols.

The first report of DR acting through a sensory, cell non-autonomous signaling mechanism in worms was in 2007. They identify the antioxidant response transcription factor skinhead 1 (SKN-1) in ASI sensory neurons as leading to increased whole-body respiration and extended lifespan (Bishop and Guarente, 2007). This report remains foundational in establishing food perception, or lack thereof, as a major driver of the health benefits of DR. More recent studies corroborate the significance of sensory neurons when nematodes are subjected to long- and short-term starvation. A subset of sensory neurons, the ASI and ASJ, shorten lifespan through the expression of insulin-like peptide (ILP) ins-6 during food perception (Artan et al., 2016). More specifically, ins-6 is released upon feeding, and ins-6 overexpression exclusively in the ASI or ASJ neurons blocks the longevity effects of tax-2; tax-4 mutant worms when fasted. This suggests that an unknown inverse signal may suppress ins-6 expression during fasting.

Sensory cues from food perceived by the nervous system trigger a host of behavioral and metabolic rearrangements that accompany changes in lifespan. Particularly, in the absence of food, nematodes tend to increase their movement when foraging and stop pharyngeal pumping (You et al., 2008; McCloskey et al., 2017; Waggoner et al., 1998). Once an attractive odorant is perceived, serotonin is released triggering triglyceride fat catabolism by a predicted acyl-CoA oxidase, acox-1 (Srinivasan et al., 2008). Surprisingly, this fat catabolism is necessary and sufficient to modulate changes in behavior. acox-1 mutants do not respond with the canonical behavioral changes observed when animals are exposed to exogenous serotonin (Zheng et al., 2018). Additionally, rescuing acox-1 expression in the intestine alone abrogates the fat accumulation and pumping response to serotonin exposure. egl-2, an ether-à-go-go (EAG) K+ channel expressed in the sensory neurons, likely functions as an intermediary neuron signaling back to serotonin neurons communicating satiety from peripheral tissues. Intriguingly, antagonizing serotonin signaling through an atypical antidepressant mianserin extends lifespan and is non-additive with DR (Petrascheck et al., 2009). It is worth exploring whether the changes in metabolism and lifespan when serotonin signaling is antagonized are acting within the same pathway. These results corroborate the hypothesis that a feed-forward signal is released from the intestine back to the nervous system during food perception (see section Emerging concepts).

Recognition of low-oxygen conditions or genetic stabilization of the conserved hypoxia-inducible factor-1 (HIF-1) extends nematode longevity (Mehta et al., 2009; Leiser et al., 2013). A single protein, flavin-containing monooxygenase-2 (FMO-2), is necessary and sufficient to provide many of the benefits of HIF-1 activation (Leiser et al., 2015). Neuronal stabilization of HIF-1 is sufficient to induce intestinal fmo-2 and improve health and longevity. Serotonergic signaling is required for HIF-1-mediated longevity and fmo-2 induction, while FMO-2 overexpression in the intestine is sufficient to increase lifespan. Interestingly, other researchers have found FMO induction in multiple mammalian models with increased lifespan, including DR, consistent with FMOs playing a conserved role in promoting long-term health and increasing the likelihood these results will be translatable to human longevity (Swindell, 2009; Steinbaugh et al., 2012).

Together, perception of many environmental signals influence lifespan in C. elegans. While the mode of sensory detection varies between these environmental signals, perception of temperature, oxygen, food availability, and mechanosensory cues all activate sensory neuron-initiated signaling pathways. This external perception interacts with internal cues (e.g. germline signal, proteostasis, and metabolism) to appropriately respond and modify physiology. While we have begun to identify the cells and signals involved in these sensory-driven longevity pathways, many questions remain regarding how information is transmitted and interpreted both within and between tissues.

Soon after the foundational discovery that reduced insulin signaling increases nematode lifespan, experiments in Drosophila revealed that such results were not worm-specific and that this pathway may be involved in modulating aging across taxa (Kannan and Fridell, 2013). Mutations disrupting molecules in this pathway such as the single insulin receptor, dInR, or the fly homolog of the insulin receptor substrate, chico, exert non-autonomous effects on aging, where reduced insulin/IGF-1 signaling (IIS) is associated with extended lifespan (Clancy et al., 2001; Tatar et al., 2001). Genetic manipulations that mimic reduced IIS, such as overexpression of dFOXO in the abdominal or pericerebral fat body or overexpression of phosphatase, dPTEN, in the pericerebral fat body also extend fly lifespan (Giannakou et al., 2004; Hwangbo et al., 2004). These data indicate that insulin signaling acts cell non-autonomously to control aging and promotes bi-directional signaling between peripheral tissues and neurons like what is seen in C. elegans (Figure 4). 

The Drosophila melanogaster genome encodes several insulin‐like peptide genes (dilps), which are produced in a handful of neurosecretory cells in the pars intercerebralis region of the fly brain. These insulin producing cells, called IPCs, release the signaling molecules into the hemolymph to signal to the rest of the body through a single insulin‐like receptor (InR) (Brogiolo et al., 2001; Colombani et al., 2012; Grönke et al., 2010). Among their range of biological functions in development and physiology, dilps modulate aging, and in this context, the most well studied are dilp2, dilp3, dilp5, and dilp6 (Bai et al., 2012; Broughton et al., 2010; Grönke et al., 2010). Partial ablation of IPCs, the production site of DILPs 2, 3, and 5 reduces IIS and increases lifespan (Broughton et al., 2005; Haselton et al., 2010). Knocking-down GABA-B receptors in IPCs decreases DILP secretion in fed and fasted conditions and yields a small but significant decrease in stress resistance and lifespan under starvation (Enell et al., 2010). Mutation of dilp2, dilp3, and dilp5 together increases lifespan as does loss of dilp2 alone, although loss of other individual dilps does not (Grönke et al., 2010). Induction of dilp6 in fat body tissue promotes longevity. It is unclear whether this is a direct effect of DILP6 or is due to a decrease in the secretion of DILP2 from the IPCs (Bai et al., 2012). Similarly, the extended lifespan observed following increased dFOXO expression in pericerebral fat body may also result from a decrease in dilp2 expression in the IPCs (Wang et al., 2005). More recent work has revealed a role for dilp1 in promoting lifespan (Post et al., 2019), potentially through induction of adipokinetic hormone (AKH), which is a functional homolog of mammalian glucagon (Bednarova et al., 2013) and which increases fly lifespan, fat metabolism, and free fatty acid catabolism (Waterson et al., 2014; Waterson et al., 2015).

The link between lifespan extension from manipulation of neuronal dilps and the insulin-responsive transcription factor FOXO in peripheral tissues are less clear in Drosophila as in C. elegans, suggesting that IIS extends lifespan, at least partly, through FOXO‐independent pathways. Loss of dilp2 does not influence the expression of known FOXO target genes and interactions with dilp1 do not modify this result (Post et al., 2019). Flies lacking IPCs or with loss of dilps 2, 3, and 5 exhibit an abnormal response to diet manipulation (Grönke et al., 2010; Broughton et al., 2010), although dFOXO mutation leaves the response largely intact (Giannakou et al., 2008). Furthermore, loss of dFOXO only partially rescues longevity benefits of chico mutants (Yamamoto and Tatar, 2011).

Another nutrient-sensing pathway that acts cell non-autonomously in flies, like worms, is AMPK. Neuronal or intestinal activation of AMPK or Atg1 induces autophagy in both the brain and gut to slow organismal aging and improves numerous healthspan measures (Ulgherait et al., 2014). Dilps are implicated in mediating the inter-tissue response from the nervous system to the intestines and vice versa (Ulgherait et al., 2014). It is interesting that AMPK activation from several tissues causes metabolic remodeling across the whole-body. These data point to a feed-forward mechanism where signaling events occur bi-directionally to modulate the fly response to successfully survive stressful stimuli.

When it was first discovered that knocking-down mitochondrial electron transport chain components in nematodes extends lifespan, it seemed unlikely this phenomenon would be conserved in higher systems. Surprisingly, research using flies and mice (later discussed in section Mammals) points to a significant role for mitochondrial function in organismal lifespan. Global knockdown of ETC components in complexes I, III, IV, and V extends fly lifespan, but does not inhibit ETC complex formation or ATP production (Copeland et al., 2009). Furthermore, knockdown of complexes I and IV in neurons alone is sufficient to extend lifespan. This led researchers to ask how knockdown of the ETC extends lifespan. Follow-up studies show that knock-down of ETC complex I using ND75 RNAi in muscle tissue increases reactive oxygen species (ROS) and activates the mito-UPR and ImpL2 (insulin/IGF binding protein) (Owusu-Ansah et al., 2013). Subsequently, upregulation of mito-UPR target genes preserve muscle function while ImpL2 signals to the brain and fat body to decrease global insulin signaling. It is likely both pathways contribute to the longevity phenotype of decreased respiration chain expression, and there are data suggesting that ImpL2 increases lysosomal biogenesis and that autophagy genes are necessary for ND75 knock-down animals to live long. Another datapoint that suggests conservation of this pathway is that knock-down of ETC ND75 in complex I results in smaller flies, and a similar phenotype is documented in the nematode ortholog isp-1 mutants (Feng et al., 2001).

Modifying the mitochondrial proton gradient by expressing human uncoupling proteins (hUCPs) modulates fly lifespan. Interestingly, the context of when and where the hUCPs are expressed play a critical role in health and longevity outcomes. hUCP2 targeted to the neurons increases health and longevity while increasing the rate of glycolysis and decreasing ROS production and oxidative damage (Fridell et al., 2005). Similarly, moderate pan-neuronal overexpression of hUCP3 leads to a modest lifespan extension exclusively in male flies (Humphrey et al., 2009). However, use of a stronger driver that targets hUCP3 pan-neuronally or to the median neurosecretory cells (mNSC) significantly decreases their lifespan. These data suggest that lowering uncoupling mitochondria by high expression of hUCP3 alters mNSC function in a way that increases DILP levels in fly heads and leads to a concomitant decrease in lifespan. Much is left to be done to fully understand the relationship between modulating mitochondria ROS levels and lifespan.

An important and robust area of research is focused on muscle maintenance with age. While slightly tangential to this review’s primary focus on longevity outcomes, the loss of muscle mass often precedes other age-related phenotypes like risks of falling. Throughout life, a fly’s muscles accumulate protein aggregates that impair function. Maintenance of proteostasis is enhanced in long-lived animals through elevated activity of FOXO target genes like 4E-BP that increase lysosome/autophagy functions (Demontis and Perrimon, 2010). Interestingly, FOXO signaling through 4E-BP activity in muscle decreases feeding behavior and the release of insulins that delay the age-related accumulation of protein aggregates in other tissues. This result suggests bi-directional cell non-autonomous signaling across tissues with a yet to be discovered signal (Demontis and Perrimon, 2010).

Overexpression of the gene hedgehog (hh), the Hedgehog signaling pathway ligand in Drosophila, extends lifespan, while disrupting this pathway shortens lifespan and decreases the number of dopaminergic neurons (Rallis et al., 2020). While overexpression of hedgehog signaling components in neurons has little effect on lifespan, overexpression in glia cells is sufficient to extend it. This work parallels nicely with work in C. elegans described above in which glial cells modulate aging through the UPR (Frakes et al., 2020). Overexpressing hsp69 and hsp40 in glia is sufficient to rescue lifespan in hh signaling mutants. Overexpression of smoothened (smo) and hsp68 in glia partially rescues the lifespan shortening effects of expressing human Αβ42 plaques in Drosophila glia. Hh signaling may increase chaperone protein expression in adult glia, which act to maintain the integrity of dopaminergic neurons, leading to increased longevity (Rallis et al., 2020). While such data are compelling, it remains unclear how these neurons contribute to the lifespan extension of enhanced hedgehog signaling. It is also interesting to consider the conserved role glia cells play in neurotransmission, cell non-autonomous signaling, and longevity (Figure 5).

Research in Drosophila has shepherded a significant expansion in our understanding of the effects of sensory perception on aging. Sensory inputs that relate information about nutrition, conspecifics, and danger rapidly initiate changes in fly physiology and patterns of aging, often within a few days. It is known, for example, that a restricted set of olfactory and gustatory neurons influence aging by either promoting or limiting lifespan, fat deposition, or general vigor in old age. Sensory perception of specific sugars and amino acids, as well as social signals such as the health of conspecifics or availability of potential mates, is also important. Conserved neuropeptides and functionally defined neuronal populations, some associated with psychological conditions such as reward and hunger, are involved in mediating these effects through new candidate cell-nonautonomous aging mechanisms (Figure 6).

Much of this work is centered around the effects of food perception. Exposure of flies to the odor of an important food source (live yeast) as well as knock-out of a critical co-factor for normal olfactory function (Or83b/Orco) established food perception is sufficient to partially reverse DR-mediated longevity (Libert et al., 2007). Loss of Orco function significantly increases fly lifespan. These effects are independent of food intake, activity, respiration, or early-life reproduction, suggesting a direct effect of sensory perception. Mutation of the water sensor, ppk28, extends Drosophila lifespan by up to 40%, and this effect requires AKH receptor (Waterson et al., 2014). AKH protein levels are higher in ppk28 mutant animals, and activation of Akh-expressing neurons is sufficient to recapitulate the effects of loss of ppk28 on lifespan. Gustatory perception is necessary for normal stress resistance and lifespan in a low-glucose environment (Linford et al., 2015). Loss of sweet taste receptor Gr64 produces a sleep-impairment phenotype that is phenocopied by blocking dopamine neurotransmission, and taste-blind flies lived longer than control flies, despite eating more (Ostojic et al., 2014). Loss of the Drosophila trehalose receptor, Gr5a, significantly decreases lifespan without altering feeding (Waterson et al., 2015), establishing that taste inputs can modulate lifespan in both directions. Similar to some methods of diet restriction in C. elegans, loss of labellar taste bristles requires insulin signaling to extend lifespan (Ostojic et al., 2014).

In Drosophila, the effects of dietary restriction on aging are predominantly influenced by dietary composition (mainly protein content) rather than the overall caloric content of the food (Solon-Biet et al., 2014; Mair et al., 2005; Skorupa et al., 2008; Simpson and Raubenheimer, 2007). Flies exposed to restriction of essential amino acids behaviorally switch from a diet comprised primarily of sugar to one primarily of protein (Steck et al., 2018). This behavioral switch in feeding preference requires both serotonin signaling through the 5-HT2A receptor and plasticity of a dopaminergic circuit (Ro et al., 2016; Liu et al., 2017). When the two primary macronutrients in the diet, sugar and protein, are presented separately to flies so that they behaviorally construct the composition of their own diet, they live shorter than when presented with a single, complete diet (Ro et al., 2016) an effect that also requires serotonin signaling through the serotonin receptor 5-HT2A. This suggests that protein sensing may be mediating this effect. Finally, serotonin, 5-HT2A, and the solute carrier 7-family amino acid transporter, JHL-21, modulate diet-dependent aging by ascribing value to ingested protein. Interestingly, JhI-21 is expressed in the reproductive tissues, which are a primary consumer of dietary protein, suggesting an inter-tissue communication.

Outside of a flies’ ability to assess food quality, several other sensory cues rely on neuronal signaling to modulate lifespan. Exposure to female sex pheromones in the absence of mating causes rapid and reversible declines in fat stores, stress resistance and longevity in male flies (Gendron et al., 2014). Changes in metabolism and lifespan require taste perception through the gustatory receptor, ppk23, as well as neuronal signaling involving the conserved neuropeptide NPF/NPY and FOXO. These effects are partially reversed by copulation, suggesting that survival costs of reproduction in male flies are controlled by neural circuits through which reproductive expectation dictates costly precopulatory investment in reproductive success. Related circuits that perceive reproductive reward ameliorate the consequences of this investment if mating is achieved (Harvanek et al., 2017). Notably, mating decreases both worm and fly lifespan, it is worthwhile to ponder why perception of imminent mating without achieving it causes greater physiological harm to a flies’ health. Perhaps upon completing a satisfying activity that is evolutionarily beneficial, like eating or mating, specific neural circuits and signaling peptides reinforce these behaviors, and without these signals only detrimental effects remain.

Cues that putatively signal danger are also important. Drosophila can visually perceive dead conspecifics in their environment and this perceptive experience induces both short- and long-term effects on health and longevity. Exposure to dead flies decreases resistance to starvation, depletes lipid storage, and shortens lifespan (Chakraborty et al., 2019). As with protein perception, serotonin signaling via receptor 5-HT2A is required for death perception to influence lifespan. With the advent of new technologies, it would be interesting to test whether the same or different neuron populations require 5-HT2A receptors to influence death and/or food perception. Gr63a encodes for one of two proteins which make up a CO₂ receptor, and at low concentrations CO₂ is a known alarm cue (Suh et al., 2007). Flies with a loss-of-function mutation in Gr63a are long-lived and are additive with ab1C neuronal ablation suggesting DR and alarm-sensing act through distinct pathways to influence lifespan (Poon et al., 2010).