LOCUS Exported 10281 bp ds-DNA circular SYN 04-JUN-2020 DEFINITION . ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10281) AUTHORS rvieira TITLE Direct Submission JOURNAL Exported Thursday, Jun 4, 2020 from SnapGene 5.1.3 https://www.snapgene.com FEATURES Location/Qualifiers source 1..10281 /organism="synthetic DNA construct" /mol_type="other DNA" /note="color: #ffffff" promoter 31..135 /gene="bla" /label=AmpR promoter /note="color: #ffffff; direction: RIGHT" CDS 136..996 /codon_start=1 /gene="bla" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /note="This feature has 2 segments: 1: 136 .. 204 / #ccffcc / signal sequence 2: 205 .. 996 / #ccffcc Cleavage site after base 204" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(354..373) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" /note="color: black; sequence: ATAATACCGCGCCACATAGC" rep_origin 1167..1755 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" primer_bind 1656..1675 /label=pBR322ori-F /note="pBR322 origin, forward primer" /note="color: black; sequence: GGGAAACGCCTGGTATCTTT" protein_bind 1842..1911 /label=attB /bound_moiety="phage phi-C31 integrase" /note="attB site for the phi-C31 integrase (Groth et al.,2000)" /note="color: #31849b" primer_bind complement(2136..2154) /label=pCasper-F /note="Drosophila mini-white gene, reverse primer" /note="color: black; sequence: GGGTTTTATTAACTTACAT" CDS join(2606..2677,3079..3352,3427..4081,4143..4458,4662..4793, 4864..5478) /codon_start=1 /gene="white" /product="Drosophila white gene eye color pigment" /label=mini-white /note="This is a modified version of the white gene lackingpart of the first intron." /note="This feature has 6 segments: 1: 2606 .. 2677 / #ffffff 2: 3079 .. 3352 / #ffffff 3: 3427 .. 4081 / #ffffff 4: 4143 .. 4458 / #ffffff 5: 4662 .. 4793 / #ffffff 6: 4864 .. 5478 / #ffffff" /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE" protein_bind 6508..6602 /label=5X UAS /bound_moiety="GAL4" /note="five tandem copies of the ""ScaI site"" 17-mer CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) that efficiently binds yeast Gal4 (Webster et al., 1988; Pfeiffer et al., 2010)" /note="color: #31849b" protein_bind 6636..6730 /label=5X UAS /bound_moiety="GAL4" /note="five tandem copies of the ""ScaI site"" 17-mer CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) that efficiently binds yeast Gal4 (Webster et al., 1988; Pfeiffer et al., 2010)" /note="color: #31849b" protein_bind 6764..6858 /label=5X UAS /bound_moiety="GAL4" /note="five tandem copies of the ""ScaI site"" 17-mer CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) that efficiently binds yeast Gal4 (Webster et al., 1988; Pfeiffer et al., 2010)" /note="color: #31849b" protein_bind 6892..6986 /label=5X UAS /bound_moiety="GAL4" /note="five tandem copies of the ""ScaI site"" 17-mer CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) that efficiently binds yeast Gal4 (Webster et al., 1988; Pfeiffer et al., 2010)" /note="color: #31849b" promoter 7011..7249 /label=hsp70 promoter /note="Drosophila melanogaster hsp70Bb promoter" /note="color: #ffffff; direction: RIGHT" promoter 7011..7249 /label=hsp70 promoter /note="Drosophila melanogaster hsp70Bb promoter" /note="color: #ffffff; direction: RIGHT" primer_bind 7196..7217 /label=pCasper-hs /note="Drosophila Hsp70 promoter, forward primer" /note="color: black; sequence: GCAACTACTGAAATCTGCCAAG" intron 7264..7330 /label=Mhc intron /note="modified Drosophila myosin heavy chain intron 16 (Pfeiffer et al., 2010)" /note="color: #------" CDS 7344..9479 /codon_start=1 /label=GluCl-alpha /note="This feature has 5 segments: 1: 7344 .. 8578 / #993366 / GluCl-alpha 2: 8579 .. 8611 / #808080 / Linker 3: 8612 .. 9335 / #00ff00 / GFP 4: 9336 .. 9371 / #808080 / Linker 5: 9372 .. 9479 / #993366 / GluCl-alpha" /translation="MGSGHYFWAILYFASLCSASLANNAKINFREKEKKVLDQILGAGK YDARIRPSGINGTDGPAIVRINLFVRSIMTISDIKMEYSVQLTFREQWTDERLKFDDIQ GRLKYLTLTEANRVWMPDLFFSNEKEGHFHNIIMPNVYIRIFPNGSVLYSIRISLTLAC PMNLKLYPLDRQICSLRMASYGWTTNDLVFLWKEGDPVQVVKNLHLPRFTLEKFLTDYC NSKTNTGEYSCLKVDLLFKREFSYYLIQIYIPCCMLVIVSWVSFWLDQGAVPARVSLGV TTLLTMATQTSGINASLPPVSYTKAIDVWTGVCLTFVFGALLEFALVNYASRSDMHKEN MKKKRRDLEQASLDAASDLLDTDSNATFAMKPLVRHPGDPLALEKRLQCEVHMQAPKRP NCCKTWLSKFPTRGGSGGSGGSGGSGSVSKGEELFTGVVPILVELDGDVNGHKFSVRGE GEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEG YVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNV YITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSK DPNEKRDHMVLLEFVTAAGITLGMDELYKGGSGGSGGSGGSSKRIDVISRITFPLVFAL FNLVYWSTYLFREEEDE" misc_feature 8578 /label=Exon Boundary /note="color: #ff0000" intron 9564..9629 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" /note="color: #------" CDS 9759..9779 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus40) large T antigen" /label=SV40 NLS /note="color: #993366" /translation="PKKKRKV" polyA_signal 10051..10185 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" /note="color: #a6acb3" polyA_signal 10051..10185 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" /note="color: #a6acb3" primer_bind complement(10088..10107) /label=SV40pA-R /note="SV40 polyA, reverse primer" /note="color: black; sequence: GAAATTTGTGATGCTATTGC" primer_bind 10142..10161 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" /note="color: black; sequence: GTGGTTTGTCCAAACTCATC" primer_bind complement(10227..10245) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forwardprimer" /note="color: black; sequence: AATAGGCGTATCACGAGGC" ORIGIN 1 gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 61 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 1021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 1081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 1141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 1201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 1261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 1321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 1381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 1441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 1501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 1561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 1621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 1681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 1741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 1801 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg 1861 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc 1921 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag 1981 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg 2041 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg 2101 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt 2161 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta 2221 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat 2281 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc 2341 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt 2401 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa 2461 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct 2521 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca 2581 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg 2641 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat 2701 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtct gcggcgtgag 2761 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag 2821 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag 2881 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat 2941 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga 3001 tacttcgggg tactttttgt tcggggttag atgagcataa cgcttgtagt tgatatttga 3061 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc 3121 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt 3181 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt 3241 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta 3301 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt 3361 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa 3421 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg 3481 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat 3541 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca 3601 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc 3661 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc 3721 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg 3781 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg 3841 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct 3901 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca 3961 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga 4021 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct 4081 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc 4141 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg 4201 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat 4261 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag 4321 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc 4381 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg 4441 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat 4501 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt 4561 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact 4621 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg 4681 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca 4741 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc 4801 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt 4861 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc 4921 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca 4981 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc 5041 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta 5101 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata 5161 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa 5221 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg 5281 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg 5341 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg 5401 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg 5461 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc 5521 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg 5581 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca 5641 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc 5701 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca 5761 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg 5821 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact 5881 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca 5941 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat 6001 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt 6061 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt 6121 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca 6181 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg 6241 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact 6301 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca 6361 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca 6421 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca 6481 gggccgcaag cttgcatgcc tgcaggtcgg agtactgtcc tccgagcgga gtactgtcct 6541 ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg 6601 agcggagact ctagccctag ggcatgcctg caggtcggag tactgtcctc cgagcggagt 6661 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact 6721 gtcctccgag cggagactct agcgctagcg catgcctgca ggtcggagta ctgtcctccg 6781 agcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc 6841 ggagtactgt cctccgagcg gagactctag cactagtgca tgcctgcagg tcggagtact 6901 gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc 6961 ctccgagcgg agtactgtcc tccgagcgga gactctagcg acgtcgagcg ccggagtata 7021 aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc 7081 gctaagcgaa agctaagcaa ataaacaagc gcagctgaac aagctaaaca atctgcagta 7141 aagtgcaagt taaagtgaat caattaaaag taaccagcaa ccaagtaaat caactgcaac 7201 tactgaaatc tgccaagaag taattattga atacaagaag agaactctga atagatctaa 7261 aaggtaggtt caaccactga tgcctaggca caccgaaacg actaacccta attcttatcc 7321 tttacttcag gcggccgcat tatatgggca gcggacacta tttctgggcg atcttatact 7381 ttgccagcct gtgcagtgct tcactagcaa ataatgccaa aataaatttc cgagaaaagg 7441 agaaaaaagt cttagatcaa attttaggtg caggcaaata cgacgcccga atacgaccat 7501 ctggaataaa tggcacagat ggtcccgcca tagtcagaat caatctattc gttcgcagta 7561 ttatgacgat tagtgatatt aaaatggagt acagtgtgca gttaaccttc cgtgaacagt 7621 ggacggatga acgcctcaag ttcgacgata tccagggtcg cctaaagtat ctgaccctga 7681 cggaggcgaa ccgcgtgtgg atgcccgatc ttttcttctc gaacgagaag gagggacact 7741 tccacaacat catcatgccc aatgtgtata ttcgcatctt ccccaacgga tctgtgctat 7801 atagtatacg tatctcgctg acattggcct gcccaatgaa cctaaagctg tatccgctgg 7861 atagacagat ctgctcacta cggatggcca gctatggctg gaccaccaac gacttggtct 7921 tcctgtggaa ggagggcgat cccgtacagg tggtaaagaa cttacaccta cctcgcttca 7981 cactggagaa gtttctgact gattactgta acagtaaaac caacaccggt gaatacagtt 8041 gcctcaaagt cgatctacta ttcaagcgag aattctcata ttacttaata caaatttata 8101 taccatgctg tatgttggtc attgtatcat gggtatcatt ctggctggat caaggagcag 8161 taccggcgcg agtgtcactg ggtgtcacca ccctgctgac catggccacc cagacgtcgg 8221 gcataaacgc ctccctgccg cccgtttcct atacgaaggc catcgatgtg tggacaggcg 8281 tgtgtctgac gttcgtgttc ggggccctgc tcgagttcgc cctggtgaac tatgcatccc 8341 gatcagacat gcataaggag aatatgaaaa agaagcgccg cgatctggag caggccagtt 8401 tagatgccgc ttcagatctg ctagatacag atagcaatgc aacgttcgca atgaaaccgt 8461 tagtacgcca tccgggcgat ccgctggccc tggaaaagcg gctccaatgc gaggtgcaca 8521 tgcaggcccc gaagcgacca aactgctgca agacctggct gtccaagttc cccacaaggg 8581 gcggaagcgg cggaagcggc ggaagcggcg gaagcggatc cgtgtccaag ggcgaggagc 8641 tgttcaccgg cgtggtgccc atcctggtgg agctggatgg cgacgtgaac ggccacaagt 8701 tcagcgtgcg cggcgagggc gagggcgacg ccaccaacgg caagctgacc ctgaagttca 8761 tctgcaccac cggcaagctg cccgtgccct ggcccaccct ggtgaccacc ctgacctacg 8821 gcgtgcagtg cttcagccgc taccccgatc acatgaagca gcacgatttc ttcaagagcg 8881 ccatgcccga gggctacgtg caggagcgca ccatcagctt caaggatgac ggcacctaca 8941 agacccgcgc cgaggtgaag ttcgagggcg ataccctggt gaaccgcatc gagctgaagg 9001 gcatcgattt caaggaggat ggcaacatcc tgggccacaa gctggagtac aacttcaaca 9061 gccacaacgt gtacatcacc gccgataagc agaagaacgg catcaaggcc aacttcaaga 9121 tccgccacaa tgtggaggat ggctccgtgc agctggccga tcactaccag cagaacaccc 9181 ccatcggcga cggcccagtg ctgctgcccg ataaccacta cctgagcacc cagagcgtgc 9241 tgtccaagga ccccaacgag aagcgcgatc acatggtgct gctggagttc gtgaccgccg 9301 ccggcatcac cctgggcatg gatgagctgt acaagggcgg aagcggcgga agcggcggaa 9361 gcggcggaag ctccaagaga atcgatgtta tatcgcggat caccttcccg ctggtcttcg 9421 ccctgttcaa cctggtctac tggagcacat atctcttcag ggaggaggag gatgagtaat 9481 tctagaggat ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac 9541 ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac 9601 tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc 9661 agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg 9721 atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag 9781 aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta 9841 atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca 9901 agaaaattat ggaaaaatat ttgatgtata gtgccttgac tagagatcat aatcagccat 9961 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg 10021 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac 10081 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 10141 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcgatct ggccggccgt 10201 ttaaacgaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt 10261 catgataata atggtttctt a //