LOCUS       Exported               10764 bp ds-DNA     circular SYN 04-JUN-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10764)
  AUTHORS   rvieira
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 4, 2020 from SnapGene 5.1.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10764
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     promoter        31..135
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             136..996
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 136 .. 204 / #ccffcc / signal sequence
                        2: 205 .. 996 / #ccffcc
                     Cleavage site after base 204"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(354..373)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="color: black; sequence: ATAATACCGCGCCACATAGC"
     rep_origin      1167..1755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     1656..1675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="color: black; sequence: GGGAAACGCCTGGTATCTTT"
     protein_bind    1842..1911
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et 
                     al.,2000)"
                     /note="color: #31849b"
     primer_bind     complement(2136..2154)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
                     /note="color: black; sequence: GGGTTTTATTAACTTACAT"
     CDS             join(2606..2677,3079..3352,3427..4081,4143..4458,4662..4793,
                     4864..5478)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene 
                     lackingpart of the first intron."
                     /note="This feature has 6 segments:
                        1: 2606 .. 2677 / #ffffff
                        2: 3079 .. 3352 / #ffffff
                        3: 3427 .. 4081 / #ffffff
                        4: 4143 .. 4458 / #ffffff
                        5: 4662 .. 4793 / #ffffff
                        6: 4864 .. 5478 / #ffffff"
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    6508..6602
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
                     /note="color: #31849b"
     protein_bind    6636..6730
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
                     /note="color: #31849b"
     protein_bind    6764..6858
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
                     /note="color: #31849b"
     protein_bind    6892..6986
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
                     /note="color: #31849b"
     promoter        7011..7249
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     7196..7217
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
                     /note="color: black; sequence: GCAACTACTGAAATCTGCCAAG"
     intron          7264..7330
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
                     /note="color: #------"
     CDS             7351..9963
                     /codon_start=1
                     /label=Rdl
                     /note="This feature has 5 segments:
                        1: 7351 .. 8103 / #993366 / Rdl
                        2: 8104 .. 8139 / #999999 / Linker
                        3: 8140 .. 8861 / #00ff00 / GFP
                        4: 8862 .. 8895 / #999999 / Linker
                        5: 8896 .. 9963 / #993366 / Rdl"
                     /translation="MSDSKMDKLARMAPLPRTPLLTIWLAINMALIAQETGHKRIHTVQ
                     AATGGGSMLGDVNISAILDSFSVSYDKRVRPNYGGPPVEVGVTMYVLSISSLSEVKMDF
                     TLDFYFRQFWTDPRLAYRKRPGVETLSVGSEFIKNIWVPDTFFVNEKQSYFHIATTSNE
                     FIRVHHSGSITRSIRLTITASCPMNLQYFPMDRQLCHIEIESFGYTMRDIRYKWNEGPN
                     SVGVSSEVSLPQFKVLGHRQRAMEISLTTGGSGGSGGSGGSGSVSKGEELFTGVVPILV
                     ELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYP
                     DHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDG
                     NILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPV
                     LLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGSGGSGGSGGSGN
                     YSRLACEIQFVRSMGYYLIQIYIPSGLIVIISWVSFWLNRNATPARVALGVTTVLTMTT
                     LMSSTNAALPKISYVKSIDVYLGTCFVMVFASLLEYATVGYMAKRIQMRKQRFMAIQKI
                     AEQKKQQLDGANQQQANPNPNANVGGPGGVGVGPGGPGGPGGGVNVGVGMGMGPEHGHG
                     HGHHAHSHGHPHAPKQTVSNRPIGFSNIQQNVGTRGCSIVGPLFQEVRFKVHDPKAHSK
                     GGTLENTVNGGRGGPQSHGPGPGQGGGPPGGGGGGGGGGGPPEGGGDPEAAVPAHLLHP
                     GKVKKDINKLLGITPSDIDKYSRIVFPVCFVCFNLMYWIIYLHVSDVVADDLVLLGEE"
     primer_bind     8263..8280
                     /label=seq_EGFP_P1 fw
                     /note="color: black; sequence: GGCAAGCTGACCCTGAAG; added: 
                     2019-07-08"
     primer_bind     complement(8732..8749)
                     /label=seq_EGFP_P1 rv
                     /note="color: black; sequence: TCAGGTAGTGGTTATCGG; added: 
                     2019-07-08"
     misc_feature    8896
                     /label=Possible EGFP insertion
                     /note="color: #ff6600"
     intron          10047..10112
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /note="color: #------"
     CDS             10242..10262
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian 
                     virus40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     polyA_signal    10534..10668
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(10571..10590)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
                     /note="color: black; sequence: GAAATTTGTGATGCTATTGC"
     primer_bind     10625..10644
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
                     /note="color: black; sequence: GTGGTTTGTCCAAACTCATC"
     primer_bind     complement(10710..10728)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, 
                     forwardprimer"
                     /note="color: black; sequence: AATAGGCGTATCACGAGGC"
ORIGIN
        1 gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
       61 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
      721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
      781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
      961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     1081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1801 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg
     1861 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc
     1921 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag
     1981 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg
     2041 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg
     2101 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt
     2161 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta
     2221 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat
     2281 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc
     2341 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt
     2401 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa
     2461 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct
     2521 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca
     2581 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg
     2641 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat
     2701 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtct gcggcgtgag
     2761 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag
     2821 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag
     2881 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat
     2941 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga
     3001 tacttcgggg tactttttgt tcggggttag atgagcataa cgcttgtagt tgatatttga
     3061 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc
     3121 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt
     3181 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt
     3241 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta
     3301 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt
     3361 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa
     3421 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg
     3481 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat
     3541 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca
     3601 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc
     3661 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc
     3721 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg
     3781 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg
     3841 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct
     3901 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca
     3961 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga
     4021 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct
     4081 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc
     4141 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg
     4201 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat
     4261 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag
     4321 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc
     4381 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg
     4441 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat
     4501 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt
     4561 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact
     4621 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg
     4681 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca
     4741 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc
     4801 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt
     4861 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc
     4921 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca
     4981 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc
     5041 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta
     5101 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata
     5161 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa
     5221 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg
     5281 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg
     5341 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg
     5401 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg
     5461 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc
     5521 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg
     5581 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca
     5641 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc
     5701 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca
     5761 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg
     5821 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact
     5881 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca
     5941 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat
     6001 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt
     6061 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt
     6121 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca
     6181 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg
     6241 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact
     6301 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca
     6361 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca
     6421 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca
     6481 gggccgcaag cttgcatgcc tgcaggtcgg agtactgtcc tccgagcgga gtactgtcct
     6541 ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg
     6601 agcggagact ctagccctag ggcatgcctg caggtcggag tactgtcctc cgagcggagt
     6661 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
     6721 gtcctccgag cggagactct agcgctagcg catgcctgca ggtcggagta ctgtcctccg
     6781 agcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc
     6841 ggagtactgt cctccgagcg gagactctag cactagtgca tgcctgcagg tcggagtact
     6901 gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc
     6961 ctccgagcgg agtactgtcc tccgagcgga gactctagcg acgtcgagcg ccggagtata
     7021 aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc
     7081 gctaagcgaa agctaagcaa ataaacaagc gcagctgaac aagctaaaca atctgcagta
     7141 aagtgcaagt taaagtgaat caattaaaag taaccagcaa ccaagtaaat caactgcaac
     7201 tactgaaatc tgccaagaag taattattga atacaagaag agaactctga atagatctaa
     7261 aaggtaggtt caaccactga tgcctaggca caccgaaacg actaacccta attcttatcc
     7321 tttacttcag gcggccgcgg ctcgagcaaa atgagtgatt caaaaatgga caagctggcc
     7381 cggatggcgc ccctgccccg cacaccgctg ctaaccatct ggctggccat caacatggcc
     7441 ctgattgcac aggaaacggg ccacaaacgg atccatacag tgcaagcggc gactggcggt
     7501 ggcagcatgc tgggtgacgt aaacatatcc gctattctcg actcctttag tgttagttac
     7561 gacaaaagag taagacccaa ttacggtgga cctcctgtcg aagtcggagt caccatgtat
     7621 gtgctatcga tcagctcgct ctcagaagtg aaaatggact tcacattgga tttttacttt
     7681 cgtcaatttt ggaccgatcc tcgtttagcg tatagaaaac gacctggtgt agaaacacta
     7741 tcggttggat cagagttcat taagaatatt tgggtacctg acaccttttt tgtaaatgaa
     7801 aaacaatcat attttcacat tgcaacaacc agtaatgaat tcatacgtgt gcatcattct
     7861 ggatcgataa caagaagtat tagattgact ataaccgcat cgtgtccgat gaatctacaa
     7921 tatttcccca tggatcgcca gctgtgccac attgaaatcg aaagcttcgg ctacacgatg
     7981 cgtgacatcc gctataaatg gaacgaaggc cccaactcgg tcggtgtctc gagtgaggtt
     8041 tcgctgcccc aatttaaggt cttgggtcat cggcaaagag ctatggaaat cagtcttacc
     8101 acaggcggaa gcggcggaag tggtggatca ggtggaagtg gatccgtgtc caagggcgag
     8161 gagctgttca ccggcgtggt gcccatcctg gtggagctgg atggcgacgt gaacggccac
     8221 aagttcagcg tgcgcggcga gggcgagggc gacgccacca acggcaagct gaccctgaag
     8281 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctggtgac caccctgacc
     8341 tacggcgtgc agtgcttcag ccgctacccc gatcacatga agcagcacga tttcttcaag
     8401 agcgccatgc ccgagggcta cgtgcaggag cgcaccatca gcttcaagga tgacggcacc
     8461 tacaagaccc gcgccgaggt gaagttcgag ggcgataccc tggtgaaccg catcgagctg
     8521 aagggcatcg atttcaagga ggatggcaac atcctgggcc acaagctgga gtacaacttc
     8581 aacagccaca acgtgtacat caccgccgat aagcagaaga acggcatcaa ggccaacttc
     8641 aagatccgcc acaatgtgga ggatggctcc gtgcagctgg ccgatcacta ccagcagaac
     8701 acccccatcg gcgacggccc agtgctgctg cccgataacc actacctgag cacccagagc
     8761 gtgctgtcca aggaccccaa cgagaagcgc gatcacatgg tgctgctgga gttcgtgacc
     8821 gccgccggca tcaccctggg catggatgag ctgtacaagg gcggaagcgg aggctctggt
     8881 ggtagtggcg gtagcggaaa ttatagccgc ctggcctgcg agatccagtt cgttcgcagc
     8941 atgggctact acctgattca gatctacatc cccagcggcc tgatcgtgat tatcagctgg
     9001 gtgtccttct ggctgaatcg caacgccaca ccagctcgag tggccctggg agtgacaacc
     9061 gtgctgacca tgaccacgct gatgtccagc accaatgccg cgctgccaaa gatctcgtac
     9121 gtcaaatcga ttgacgtcta tctgggaaca tgcttcgtta tggtctttgc cagtctactg
     9181 gaatacgcca cggtcggcta catggcaaaa cgaattcaaa tgcgaaaaca aagatttatg
     9241 gcgatccaaa agatagccga acagaaaaag caacagctcg acggagcgaa ccaacagcag
     9301 gcgaatccca atcccaatgc aaatgtgggc ggacccggag gagtgggcgt tggacccggc
     9361 ggaccaggag gacccggtgg cggagtcaat gtgggcgtcg gtatgggcat gggaccggaa
     9421 catggccacg ggcatggaca ccacgcccac agccatggac atccgcatgc gcccaagcaa
     9481 acagtgagta accgcccaat cggcttttcc aatatccaac aaaacgttgg tacgcgcggt
     9541 tgctcgatag tgggaccctt gttccaggag gtgagattca aggtccacga cccgaaggcc
     9601 cactccaagg gcggaacgct ggagaatacg gtgaatggcg gacgcggtgg tccgcaatcg
     9661 catggaccgg gtccgggcca aggcggcgga ccaccaggag gtggaggagg cggtggaggc
     9721 gggggcggac cacccgaagg aggtggcgat ccggaggcag cggttccagc ccatcttctg
     9781 catccgggaa aagtaaaaaa ggacatcaac aagctgctgg gcatcacgcc ctccgacatc
     9841 gacaagtact cacgcatcgt gttccccgtg tgcttcgtgt gcttcaacct gatgtactgg
     9901 atcatttacc tgcatgtcag cgacgtggtc gccgatgatc tggtgctcct gggcgaggag
     9961 tagtctagag gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac
    10021 tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta
    10081 aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg
    10141 agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta
    10201 gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg
    10261 tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta
    10321 gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat
    10381 acaagaaaat tatggaaaaa tatttgatgt atagtgcctt gactagagat cataatcagc
    10441 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
    10501 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
    10561 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    10621 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcga tctggccggc
    10681 cgtttaaacg aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
    10741 tgtcatgata ataatggttt ctta
//
