**Data in this source file were used to generate figures 2a and figure 5 supplement 1 and 2. ERC calculations were completed using the Evolutionary Rate Covariation (ERC) web tool at https://csb.pitt.edu/erc_analysis/. Gene Ontology subontology Meiosis I (GO:0007127) was obtained from AmiGO 2 (v2.5.13).** ERC Values Column P-value Row P-value Protein ID RAD1 RAD9A RAD9B HUS1 HUS1B RAD1 RAD9A RAD9B HUS1 HUS1B RAD1 RAD9A RAD9B HUS1 HUS1B ANKRD31 -0.051 0.744 ND 0.053 ND 0.6326 0.002 ND 0.4945 ND 0.6132 0.0003 ND 0.4628 ND ATM 0.4 0.321 0.259 0.264 ND 0.0617 0.1166 0.1793 0.1739 ND 0.0517 0.1445 0.184 0.1683 ND AURKA 0.5 -0.259 ND 0.079 ND 0.014 0.9002 ND 0.4126 ND 0.0166 0.8739 ND 0.4214 ND BAG6 0.051 0.333 ND ND ND 0.4409 0.128 ND ND ND 0.4463 0.1337 ND ND ND BCL2L11 0.208 0.19 0.19 0.187 0.109 0.1873 0.2091 0.2091 0.2131 0.3239 0.215 0.2934 0.2701 0.262 0.3424 BRCA2 0.53 0.342 ND ND ND 0.0205 0.1144 ND ND ND 0.0105 0.1269 ND ND ND BRDT 0.583 -0.262 ND 0.046 ND 0.0091 0.7999 ND 0.3947 ND 0.0048 0.8764 ND 0.475 ND BRIP1 0.402 0.408 ND ND ND 0.1019 0.0985 ND ND ND 0.0509 0.0767 ND ND ND BTBD18 0.203 0.193 ND 0.249 0.132 0.248 0.262 ND 0.1888 0.3469 0.2226 0.2896 ND 0.1844 0.3013 CCDC36 0.166 0.186 0.63 ND ND 0.3014 0.2738 0.0028 ND ND 0.2709 0.2976 0.0027 ND ND CCDC79 -0.059 0.476 0.377 0.058 -0.163 0.597 0.0372 0.0838 0.4266 0.745 0.6257 0.0404 0.0789 0.4534 0.8051 CCNA1 0.071 ND ND ND ND 0.3556 ND ND ND ND 0.4112 ND ND ND ND CCNE1 0.301 0.026 0.549 0.422 0.226 0.1229 0.5259 0.0073 0.0415 0.2056 0.117 0.5208 0.0124 0.0495 0.1712 CDC25B -0.084 0.428 ND 0.21 ND 0.6875 0.0638 ND 0.2782 ND 0.6669 0.0655 ND 0.2297 ND CKS2 0.255 0.25 -0.168 0.24 0.398 0.1139 0.1167 0.7784 0.1239 0.0352 0.1619 0.2185 0.7888 0.1938 0.0321 CNTD1 -0.073 0.401 0.041 0.059 ND 0.6813 0.0729 0.499 0.4698 ND 0.6505 0.082 0.4924 0.4516 ND DDX4 0.215 -0.54 0.095 ND ND 0.1586 0.9852 0.3106 ND ND 0.2076 0.9927 0.4103 ND ND DMRT1 0.163 0.208 ND 0.006 ND 0.2347 0.1815 ND 0.4642 ND 0.2762 0.2706 ND 0.5425 ND DMRTC2 -0.339 0.491 ND -0.118 ND 0.9259 0.0452 ND 0.7106 ND 0.9396 0.0346 ND 0.7451 ND EME1 0.155 0.326 ND 0.061 ND 0.3435 0.1248 ND 0.498 ND 0.2873 0.1406 ND 0.4487 ND ERCC4 0.206 0.176 0.152 0.019 ND 0.1693 0.2108 0.2457 0.486 ND 0.2185 0.3097 0.3227 0.5189 ND ESPL1 0.052 0.456 0.281 0.166 0.155 0.5254 0.0699 0.2116 0.3613 0.3772 0.4442 0.0492 0.1592 0.2917 0.2665 FANCD2 ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND FANCM 0.217 0.298 0.331 0.169 ND 0.2436 0.1503 0.1208 0.3078 ND 0.205 0.1657 0.1119 0.2873 ND FBXO5 0.014 0.307 ND 0.302 -0.149 0.5538 0.097 ND 0.1013 0.8171 0.5089 0.1575 ND 0.1291 0.785 FMN2 0.396 -0.2 ND -0.25 ND 0.055 0.7649 ND 0.8227 ND 0.0535 0.8175 ND 0.8982 ND HFM1 0.372 0.205 0.327 0.518 0.083 0.0671 0.2287 0.1011 0.0137 0.4122 0.0663 0.2747 0.1151 0.0183 0.3873 HORMAD1 -0.17 0.403 0.063 -0.115 ND 0.7371 0.0677 0.3934 0.6581 ND 0.7907 0.0809 0.4596 0.7402 ND HSF2BP 0 0.276 ND 0.218 ND 0.5811 0.1835 ND 0.2548 ND 0.5319 0.1888 ND 0.2196 ND HSPA2 -0.008 0.278 -0.098 0.18 ND 0.4818 0.124 0.6336 0.2173 ND 0.5462 0.1867 0.6986 0.2717 ND HUS1 0.67 0.28 0.429 N/A ND 0.0016 0.1512 0.0467 N/A ND 0.0009 0.1843 0.0499 N/A ND HUS1B ND ND ND ND N/A ND ND ND ND N/A ND ND ND ND N/A ING2 0.109 -0.094 0.308 0.346 -0.131 0.2898 0.6362 0.073 0.052 0.6964 0.3531 0.6885 0.1336 0.0924 0.7577 LEPREL4 ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND M1AP -0.128 0.596 0.19 0.238 0.095 0.7574 0.0081 0.2817 0.2245 0.4173 0.7329 0.0076 0.2701 0.1965 0.3634 MAEL -0.117 -0.016 0.152 0.098 ND 0.6897 0.5278 0.2667 0.3447 ND 0.7162 0.5807 0.3227 0.3899 ND MCMDC2 -0.04 0.206 0.218 0.072 0.168 0.5955 0.1957 0.1807 0.4015 0.248 0.5958 0.2736 0.2343 0.4324 0.2475 MEI1 -0.213 0.361 0.094 0.131 0.236 0.8483 0.1168 0.4419 0.3904 0.2522 0.8399 0.1106 0.4114 0.3418 0.1583 MEIOB -0.074 0.416 -0.012 0.093 0.048 0.6496 0.0304 0.5388 0.3476 0.4281 0.6521 0.0725 0.5792 0.3988 0.449 MLH1 -0.003 0.22 0.202 0.08 ND 0.5798 0.192 0.2162 0.4241 ND 0.5368 0.2558 0.2549 0.4199 ND MLH3 -0.356 0.551 -0.142 -0.025 0.162 0.95 0.0272 0.7785 0.6304 0.3648 0.9491 0.0161 0.7568 0.5978 0.2568 MND1 0.107 -0.113 ND 0.047 0.12 0.2969 0.6833 ND 0.4024 0.2765 0.3555 0.7136 ND 0.4732 0.3229 MOV10L1 0.288 -0.096 ND 0.484 ND 0.141 0.6533 ND 0.0283 ND 0.1288 0.6917 ND 0.0274 ND MRE11A 0.244 -0.158 -0.193 0.043 ND 0.1314 0.7942 0.8416 0.4416 ND 0.1742 0.7691 0.815 0.4809 ND MSH4 0.119 0.057 0.084 -0.028 0.425 0.4078 0.5071 0.462 0.6379 0.0661 0.3389 0.4751 0.4293 0.6024 0.0244 MUS81 -0.333 -0.013 -0.598 -0.484 ND 0.9297 0.5089 0.9986 0.9876 ND 0.9362 0.5766 0.9963 0.994 ND MYBL1 0.228 0.034 0.101 0.401 0.025 0.1432 0.451 0.3269 0.03 0.4686 0.1925 0.508 0.4006 0.0599 0.4864 NDC1 -0.278 0.003 ND ND ND 0.8899 0.5514 ND ND ND 0.899 0.5536 ND ND ND PIWIL2 0.088 0.302 0.11 0.192 0.085 0.3795 0.0996 0.3419 0.2196 0.384 0.3854 0.1619 0.3859 0.2539 0.3828 PLK1 0.427 0.077 ND 0.1 ND 0.0228 0.3769 ND 0.3383 ND 0.04 0.4457 ND 0.3873 ND PSMA8 0.148 0.115 ND 0.24 ND 0.2616 0.3144 ND 0.1411 ND 0.2972 0.3899 ND 0.1938 ND PSMC3 0.514 -0.332 0.376 0.12 ND 0.0079 0.9223 0.0415 0.2854 ND 0.0137 0.9258 0.0792 0.3575 ND PSMD13 -0.071 -0.224 -0.133 0.064 -0.375 0.5771 0.828 0.6902 0.331 0.9617 0.6476 0.8421 0.7451 0.4445 0.9756 PTTG1 0.019 0.227 0.219 0.21 ND 0.5067 0.207 0.2163 0.2253 ND 0.5004 0.2457 0.2329 0.2297 ND PTTG3P 0.492 ND ND ND ND 0.0239 ND ND ND ND 0.0189 ND ND ND ND RAD1 N/A 0.017 0.486 0.67 ND N/A 0.5036 0.0206 0.0009 ND N/A 0.5342 0.0271 0.0016 ND RAD21 0.285 -0.085 0.406 0.205 ND 0.1 0.6075 0.0349 0.177 ND 0.1315 0.6771 0.0621 0.2358 ND RAD21L1 0.431 0.21 0.539 0.522 0.209 0.0415 0.2601 0.01 0.0128 0.2616 0.0385 0.2681 0.0137 0.0177 0.192 RAD50 0.144 -0.285 -0.068 -0.145 -0.221 0.2386 0.9314 0.6453 0.7807 0.8741 0.3031 0.8939 0.6604 0.7831 0.871 RAD51 0.006 0.342 -0.263 0.24 0.304 0.5406 0.0682 0.9165 0.1594 0.0971 0.5227 0.1269 0.8836 0.1938 0.0949 RAD51B 0.105 0.488 0.03 0.355 ND 0.4037 0.0213 0.5292 0.0913 ND 0.3585 0.0356 0.5098 0.0849 ND RAD51C -0.306 -0.043 ND -0.173 ND 0.9513 0.6035 ND 0.8251 ND 0.9193 0.6196 ND 0.8169 ND RAD51D -0.043 ND ND ND ND 0.6513 ND ND ND ND 0.6007 ND ND ND ND RAD54B 0.064 0.572 0.113 0.168 0.127 0.4767 0.01 0.3966 0.3075 0.3736 0.4231 0.0111 0.3813 0.2888 0.3117 RAD54L -0.294 0.266 -0.38 -0.356 ND 0.9061 0.1553 0.9611 0.9482 ND 0.9111 0.1989 0.9547 0.9647 ND RAD9A 0.017 N/A -0.129 0.28 ND 0.5342 N/A 0.7331 0.1843 ND 0.5036 N/A 0.7397 0.1512 ND RAD9B 0.486 -0.129 N/A 0.429 ND 0.0271 0.7397 N/A 0.0499 ND 0.0206 0.7331 N/A 0.0467 ND REC8 -0.227 0.227 0.09 0.002 0.288 0.8967 0.1937 0.4106 0.5716 0.1246 0.8537 0.2457 0.4186 0.5499 0.107 RMI1 -0.115 0.197 0.127 0.041 ND 0.7425 0.2806 0.383 0.5143 ND 0.7135 0.2844 0.3609 0.4843 ND SGOL1 0.126 0.408 ND ND ND 0.354 0.0544 ND ND ND 0.329 0.0767 ND ND ND SGOL2 ND 0.006 ND ND ND ND 0.5424 ND ND ND ND 0.5497 ND ND ND SIRT7 -0.056 -0.639 -0.101 0.021 ND 0.5764 0.9996 0.6619 0.4345 ND 0.621 0.9989 0.7022 0.5157 ND SLX4 -0.102 0.325 ND 0.122 ND 0.7337 0.1328 ND 0.3859 ND 0.6952 0.1414 ND 0.3551 ND SPATA22 -0.087 0.396 ND 0.082 ND 0.6947 0.0687 ND 0.4314 ND 0.6711 0.0851 ND 0.416 ND SPO11 0.009 0.283 0.109 -0.222 ND 0.5038 0.0954 0.3185 0.8693 ND 0.5172 0.1806 0.3878 0.8725 ND STAG3 0.094 0.589 0.355 0.244 0.017 0.4212 0.0066 0.1025 0.2107 0.5459 0.375 0.0089 0.0939 0.1901 0.5023 SUN1 0.325 -0.002 ND ND ND 0.1229 0.5342 ND ND ND 0.0975 0.5602 ND ND ND SYCE1 0.418 0.214 0.038 0.142 0.222 0.0524 0.252 0.539 0.3672 0.2404 0.0434 0.2633 0.4966 0.324 0.1764 SYCE1L 0.434 0.214 0.418 0.371 ND 0.0474 0.2319 0.0564 0.0841 ND 0.0371 0.2633 0.0559 0.0757 ND SYCE2 0.242 0.29 0.239 0.445 ND 0.1945 0.1418 0.1986 0.0356 ND 0.1759 0.1739 0.2087 0.0397 ND SYCE3 -0.258 0.106 -0.132 -0.108 -0.038 0.9062 0.3372 0.7525 0.713 0.5981 0.8835 0.4028 0.7438 0.7295 0.6079 SYCP1 -0.095 0.567 0.158 0.057 ND 0.6926 0.0152 0.3339 0.475 ND 0.6845 0.0119 0.3134 0.4551 ND SYCP2 0.228 0.275 0.386 0.013 -0.109 0.1858 0.1371 0.0583 0.4949 0.6802 0.1925 0.19 0.0726 0.5298 0.7263 SYCP3 -0.168 0.165 ND -0.097 ND 0.7323 0.2421 ND 0.629 ND 0.7879 0.3252 ND 0.713 ND TDRD9 0.009 0.333 0.132 0.175 0.186 0.4939 0.0798 0.2919 0.2341 0.2196 0.5172 0.1337 0.3521 0.2786 0.2206 TERF1 0.337 0.056 ND 0.496 ND 0.0967 0.4231 ND 0.021 ND 0.0903 0.4766 ND 0.0245 ND TEX11 0.47 0.451 ND 0.151 ND 0.0301 0.0376 ND 0.3249 ND 0.025 0.0518 ND 0.3115 ND TEX12 0.066 0.06 0.309 0.164 ND 0.4498 0.459 0.1191 0.2958 ND 0.4199 0.4705 0.1318 0.2947 ND TEX15 0.063 0.284 ND ND ND 0.4621 0.1701 ND ND ND 0.4254 0.1792 ND ND ND TEX19 0.413 0.328 ND 0.192 ND 0.0415 0.0926 ND 0.2407 ND 0.0453 0.1381 ND 0.2539 ND TOP2A 0.296 -0.111 ND 0.341 ND 0.1179 0.7167 ND 0.08 ND 0.1219 0.7104 ND 0.095 ND TRIP13 -0.195 0.351 -0.288 -0.037 ND 0.8316 0.0706 0.9236 0.5892 ND 0.8208 0.1196 0.9018 0.6164 ND UBE2B 0.142 -0.434 -0.219 -0.014 -0.176 0.2159 0.957 0.7516 0.4367 0.6906 0.3058 0.9718 0.8407 0.5783 0.8223 UBR2 0.506 -0.068 0.429 0.196 -0.257 0.0106 0.6265 0.0292 0.2072 0.8675 0.0152 0.6551 0.0499 0.2491 0.9063 WEE2 0.348 -0.027 0.247 0.328 -0.403 0.0854 0.5865 0.1789 0.1008 0.9642 0.0819 0.5956 0.1984 0.1064 0.9855