Target,Identifier,Target,Spacer,sgRNA,Delivery method,Corresponding plasmid (if relevant)
Human RARA C-terminus,psgAH046,Human RARA C-terminus,CATGTGGCGTGGGCGGTCAC,CAUGUGGCGUGGGCGGUCAC ccgugaccgcccacgccacaguuuuagagcuagaaauagcaaguuaaaauaaggcuaguccguuaucaacuugaaaaaguggcaccgagucggugc,Expression from nucleofected plasmid,pU6_sgRNA_CBh_Cas9_PGK_Venus_anti-RARA-C_terminus_1
Human RARA C-terminus,psgAH047,Human RARA C-terminus,CCGTGACCGCCCACGCCACA,CCGUGACCGCCCACGCCACA ccgugaccgcccacgccacaguuuuagagcuagaaauagcaaguuaaaauaaggcuaguccguuaucaacuugaaaaaguggcaccgagucggugc,Expression from nucleofected plasmid,pU6_sgRNA_CBh_Cas9_PGK_Venus_anti-RARA-C_terminus_2
Human NPM1 C-terminus,sgAH017,Human NPM1 C-terminus,TCCAGGCTATTCAAGATCTC,UCCAGGCUAUUCAAGAUCUC guuuuagagcuagaaauagcaaguuaaaauaaggcuaguccguuaucaacuugaaaaaguggcaccgagucggugcuuuuuu,sgRNP nucleofection,
Human NPM1 C-terminus,sgAH018,Human NPM1 C-terminus,GCCAGAGATCTTGAATAGCC,GCCAGAGAUCUUGAAUAGCC guuuuagagcuagaaauagcaaguuaaaauaaggcuaguccguuaucaacuugaaaaaguggcaccgagucggugcuuuuuu,sgRNP nucleofection,