time (s) elapsed after fluorescent ligand adding in FITC-LDVP concentration (M) Unlabeled ligand adding in time (s) MFI 33 0.000000005 664 112.3 69 0.000000005 664 182.3 102 0.000000005 664 238.3 134 0.000000005 664 249.3 175 0.000000005 664 256.3 215 0.000000005 664 261.3 256 0.000000005 664 265.3 289 0.000000005 664 274.3 323 0.000000005 664 260.3 355 0.000000005 664 272.3 399 0.000000005 664 269.3 431 0.000000005 664 271.3 467 0.000000005 664 267.3 499 0.000000005 664 277.3 531 0.000000005 664 272.3 562 0.000000005 664 265.3 594 0.000000005 664 274.3 688 0.000000005 664 162.3 719 0.000000005 664 91.3 751 0.000000005 664 66.3 789 0.000000005 664 34.3 822 0.000000005 664 24.3 852 0.000000005 664 16.3 883 0.000000005 664 10.3 913 0.000000005 664 1.3 950 0.000000005 664 6.3 983 0.000000005 664 2.3 1015 0.000000005 664 1.3 1046 0.000000005 664 1.3 1076 0.000000005 664 -0.7 1107 0.000000005 664 0.3 1140 0.000000005 664 -5.7 1173 0.000000005 664 0.3 1205 0.000000005 664 -3.7 1244 0.000000005 664 -0.7 27 0.00000001 657 242.7 60 0.00000001 657 325.7 95 0.00000001 657 350.7 126 0.00000001 657 374.7 158 0.00000001 657 374.7 192 0.00000001 657 382.7 224 0.00000001 657 390.7 259 0.00000001 657 400.7 295 0.00000001 657 395.7 328 0.00000001 657 390.7 360 0.00000001 657 385.7 391 0.00000001 657 400.7 427 0.00000001 657 395.7 459 0.00000001 657 390.7 496 0.00000001 657 399.7 528 0.00000001 657 385.7 560 0.00000001 657 392.7 602 0.00000001 657 400.7 683 0.00000001 657 165.7 715 0.00000001 657 88.7 748 0.00000001 657 56.7 779 0.00000001 657 31.7 810 0.00000001 657 26.7 841 0.00000001 657 18.7 876 0.00000001 657 12.7 909 0.00000001 657 12.7 942 0.00000001 657 11.7 975 0.00000001 657 6.7 1007 0.00000001 657 7.7 1040 0.00000001 657 3.7 1072 0.00000001 657 2.7 1111 0.00000001 657 5.7 1144 0.00000001 657 1.7 1175 0.00000001 657 -1.3 1209 0.00000001 657 -2.3 1239 0.00000001 657 -3.3 27 0.00000002 637 444.7 59 0.00000002 637 565.7 93 0.00000002 637 602.7 124 0.00000002 637 611.7 233 0.00000002 637 599.7 266 0.00000002 637 599.7 297 0.00000002 637 580.7 333 0.00000002 637 595.7 365 0.00000002 637 605.7 396 0.00000002 637 603.7 427 0.00000002 637 596.7 458 0.00000002 637 605.7 491 0.00000002 637 603.7 522 0.00000002 637 608.7 555 0.00000002 637 598.7 586 0.00000002 637 601.7 666 0.00000002 637 299.7 699 0.00000002 637 172.7 731 0.00000002 637 105.7 762 0.00000002 637 77.7 794 0.00000002 637 52.7 825 0.00000002 637 42.7 856 0.00000002 637 31.7 891 0.00000002 637 26.7 924 0.00000002 637 29.7 958 0.00000002 637 28.7 990 0.00000002 637 15.7 1025 0.00000002 637 11.7 1064 0.00000002 637 5.7 1095 0.00000002 637 -0.3 1127 0.00000002 637 0.7 1159 0.00000002 637 3.7 1191 0.00000002 637 1