time (s) elapsed after fluorescent ligand adding in FITC-LDVP concentration (M) Unlabeled ligand adding in time (s) MFI 71 0.000000005 935 8.5 103 0.000000005 935 16.5 135 0.000000005 935 20.5 167 0.000000005 935 19.5 206 0.000000005 935 32.5 240 0.000000005 935 31.5 274 0.000000005 935 36.5 306 0.000000005 935 36.5 340 0.000000005 935 45.5 380 0.000000005 935 51.5 415 0.000000005 935 51.5 449 0.000000005 935 61.5 483 0.000000005 935 61.5 515 0.000000005 935 63.5 550 0.000000005 935 72.5 581 0.000000005 935 74.5 613 0.000000005 935 79.5 658 0.000000005 935 82.5 690 0.000000005 935 81.5 722 0.000000005 935 88.5 756 0.000000005 935 84.5 796 0.000000005 935 94.5 862 0.000000005 935 98.5 895 0.000000005 935 108.5 1028 0.000000005 935 112.5 1071 0.000000005 935 113.5 1107 0.000000005 935 113.5 1140 0.000000005 935 114.5 1176 0.000000005 935 114.5 1209 0.000000005 935 111.5 1244 0.000000005 935 112.5 1277 0.000000005 935 110.5 1309 0.000000005 935 110.5 1346 0.000000005 935 108.5 1381 0.000000005 935 114.5 1414 0.000000005 935 113.5 1458 0.000000005 935 108.5 1490 0.000000005 935 112.5 1522 0.000000005 935 108.5 30 0.00000001 945 6 67 0.00000001 945 19 100 0.00000001 945 29 134 0.00000001 945 33 166 0.00000001 945 47 198 0.00000001 945 54 231 0.00000001 945 59 264 0.00000001 945 73 297 0.00000001 945 86 331 0.00000001 945 87 363 0.00000001 945 93 394 0.00000001 945 103 428 0.00000001 945 111 461 0.00000001 945 112 492 0.00000001 945 121 527 0.00000001 945 135 559 0.00000001 945 144 591 0.00000001 945 155 624 0.00000001 945 152 656 0.00000001 945 162 691 0.00000001 945 183 725 0.00000001 945 175 756 0.00000001 945 179 788 0.00000001 945 192 820 0.00000001 945 194 854 0.00000001 945 204 886 0.00000001 945 210 916 0.00000001 945 216 978 0.00000001 945 207 1010 0.00000001 945 204 1040 0.00000001 945 208 1071 0.00000001 945 212 1103 0.00000001 945 211 1134 0.00000001 945 216 1166 0.00000001 945 212 1197 0.00000001 945 213 1231 0.00000001 945 211 1262 0.00000001 945 208 1294 0.00000001 945 210 1327 0.00000001 945 205 1358 0.00000001 945 209 1391 0.00000001 945 212 1422 0.00000001 945 213 1455 0.00000001 945 216 28 0.00000002 974 33 71 0.00000002 974 61 107 0.00000002 974 80 145 0.00000002 974 79 177 0.00000002 974 93 218 0.00000002 974 96 256 0.00000002 974 97 296 0.00000002 974 116 330 0.00000002 974 149 364 0.00000002 974 155 399 0.00000002 974 172 432 0.00000002 974 179 466 0.00000002 974 191 499 0.00000002 974 211 537 0.00000002 974 218 572 0.00000002 974 217 605 0.00000002 974 237 638 0.00000002 974 264 671 0.00000002 974 260 732 0.00000002 974 288 765 0.00000002 974 270 830 0.00000002 974 319 937 0.00000002 974 354 1007 0.00000002 974 340 1051 0.00000002 974 352 1088 0.00000002 974 335 1124 0.00000002 974 340 1156 0.00000002 974 342 1192 0.00000002 974 346 1234 0.00000002 974 342 1267 0.00000002 974 334 1304 0.00000002 974 348 1344 0.00000002 974 319 1376 0.00000002 974 332 1424 0.00000002 974 324 1459 0.00000002 974 326 1496 0.00000002 974 322 1528 0.00000002 974 328 1564 0.00000002 974 313 1597 0.00000002 974 325 1634 0.00000002 974 311