time (s) elapsed after fluorescent ligand adding in Alexa488-Fn3 9-10 concentration (M) Unlabeled ligand adding in time (s) MFI 27 0.00000001 1072 3 200 0.00000001 1072 120 316 0.00000001 1072 165 444 0.00000001 1072 169 570 0.00000001 1072 203 687 0.00000001 1072 201 810 0.00000001 1072 213 948 0.00000001 1072 244 2333 0.00000001 1072 298 1102 0.00000001 1072 221 1253 0.00000001 1072 219 1363 0.00000001 1072 211 1520 0.00000001 1072 217 1638 0.00000001 1072 211 1782 0.00000001 1072 201 1921 0.00000001 1072 204 2050 0.00000001 1072 201 2207 0.00000001 1072 201 30 0.00000002 1065 28 174 0.00000002 1065 156 297 0.00000002 1065 200 424 0.00000002 1065 204 543 0.00000002 1065 240 662 0.00000002 1065 235 782 0.00000002 1065 254 923 0.00000002 1065 269 2311 0.00000002 1065 305 1094 0.00000002 1065 273 1225 0.00000002 1065 263 1334 0.00000002 1065 239 1494 0.00000002 1065 259 1634 0.00000002 1065 242 1765 0.00000002 1065 246 1900 0.00000002 1065 254 2030 0.00000002 1065 227 28 0.00000003 1059 58 144 0.00000003 1059 196 270 0.00000003 1059 210 396 0.00000003 1059 230 517 0.00000003 1059 265 635 0.00000003 1059 272 759 0.00000003 1059 281 902 0.00000003 1059 311 2286 0.00000003 1059 325 1086 0.00000003 1059 294 1196 0.00000003 1059 289 1342 0.00000003 1059 278 1467 0.00000003 1059 279 1609 0.00000003 1059 265 1741 0.00000003 1059 271 1875 0.00000003 1059 271 2012 0.00000003 1059 257