To measure the activation of the four families of G protein isoforms at the plasma membrane and early endosomes by the V₂R in real time, we used mG proteins. The mG proteins are homogenously distributed in the cytosol under basal condition but translocate to the subcellular location of active GPCRs upon stimulation (Carpenter and Tate, 2016; Nehmé et al., 2017; Wan et al., 2018). In addition, we applied an EbBRET approach instead of a conventional bioluminescence resonance energy transfer (BRET)-based assay to monitor mG protein trafficking. EbBRET displays superior robustness and sensitivity, as well as higher dynamic spectrometric energy transfer signals compared to conventional BRET, which is why this approach was favored (Namkung et al., 2016). We fused mG proteins to the luciferase from Renilla reniformis (Rluc) and anchored green fluorescent protein from the same species (rGFP) to the polybasic sequence and prenylation CAAX box of KRas (rGFP-CAAX), which is located at the plasma membrane (Zacharias et al., 2002), or to the early endosome marker Rab5 (Gorvel et al., 1991; Figure 1A, B, left panels). Four variants of mG proteins (mGs, mGsi, mGsq, and mG12) have been designed and shown to maintain the receptor-Gα protein specificity of the four Gα subunit isoform families (Wan et al., 2018). In HEK293 cells expressing rGFP-CAAX, V₂R, and similar levels of Rluc-fused mG proteins (Figure 1—figure supplement 1A), arginine vasopressin (AVP) treatment induced a rapid recruitment of mGs and mGsq but not mGsi nor mG12 to the plasma membrane. Maximal recruitment of the mGs and mGsq were reached ~10 min after initial stimulation (Figure 1A, right panel). These results suggest that the V₂R activates both Gα_s and Gα_q/11 at the plasma membrane. In addition, AVP stimulation led to the recruitment of the same mG protein isoforms to early endosomes in cells expressing rGFP-Rab5, V₂R, and similar levels of Rluc-fused mG proteins (Figure 1B, right panel, Figure 1—figure supplement 1B). In contrast to the plasma membrane response, mGs and mGsq recruitment to early endosomes were slower and reached maximal levels 45–60 min after initial stimulation with AVP (Figure 1B, right panel).

Activation of Gα_q/11 canonically stimulates phospholipase Cβ (PLCβ), an enzyme that hydrolyzes membrane phosphoinositides into diacylglycerol (DAG) and inositol phosphate. Consequently, to validate Gα_q/11 activation at plasma membrane and early endosomes by the V₂R, we monitored the AVP-mediated production of DAG at these subcellular compartments. To monitor DAG at the plasma membrane, we used the C1b DAG-binding domain of PKCδ fused to Rluc (Rluc-C1b) and measured the EbBRET between Rluc-C1b and rGFP-CAAX (Wright et al., 2021) upon stimulation with AVP or vehicle (Figure 1C, left panel). In line with the recruitment of mGsq at the plasma membrane observed in Figure 1A, AVP induced a recruitment of Rluc-C1b at the plasma membrane depicted by an increase of EbBRET values, which confirms the production of DAG at the plasma membrane by V₂R (Figure 1C, right panel). Importantly, this production of DAG is Gα_q/11-dependent as a pre-treatment with YM254890, an inhibitor of Gα_q/11, abrogated this downstream response (Taniguchi et al., 2003). To measure the production of DAG at early endosomes by V₂R, we monitored the EbBRET between Rluc-C1b and rGFP-Rab5 upon V₂R stimulation (Figure 1D, left panel). Similar to the observed recruitment of mGsq to the early endosomes (Figure 1B), AVP also induced a recruitment of Rluc-C1b to the early endosomal marker rGFP-Rab5, which suggests that DAG is produced at endosomal membranes (Figure 1D, right panel). This endosomal DAG production was also inhibited by YM254890 treatment, which demonstrates that it is Gα_q/11 dependent (Figure 1D, right panel). As DAG is an activator of protein kinase C (PKC), we further monitored PKC activation upon stimulation with AVP or vehicle. To measure PKC activation, we used an unimolecular BRET-based sensor composed of two Rluc-C1b-fused PKC consensus sequences (TLKI;pPKC1 and TLKD;pPKC2) that are connected to the phosphothreonine-binding domains FHA1 and FHA2 and the BRET acceptor GFP10 via a flexible linker (Namkung et al., 2018; Figure 1E, left panel). Upon phosphorylation of pPKC1 and pPKC2, FHA1 and FHA2 bind to these phosphorylated sequences resulting in an increased proximity between Rluc and GFP10 and a corresponding increase of BRET signal. In line with the AVP-mediated production of DAG, AVP treatment also induced phosphorylation of PKC in a Gα_q/11-dependent fashion (Figure 1E, right panel).

To visualize V₂R-mediated activation of Gα_s and Gα_q/11 at the plasma membrane and early endosomes we used confocal microscopy. For this purpose, we transfected HEK293 cells with mGs, mGsq, or mGsi (as negative control) fused to a HaloTag (Halo-mGs, Halo-mGsq, Halo-mGsi) along with the respective red fluorescent protein (RFP)-fused plasma membrane or early endosomes markers Lck (Ley et al., 1994) or early endosome antigen 1 (EEA1) (Simonsen et al., 1998). Upon HaloTag labeling with a fluorescent green ligand, mGs, mGsq, and mGsi were visible and homogenously distributed in the cytosol under basal condition (vehicle) (Figure 2A, upper panels). In contrast, in cells treated with AVP for 10 min, mGs and mGsq but not mGsi were redistributed along the periphery of the cells where they colocalized with Lck (Figure 2A, bottom panels, Figure 2B). These observations confirm that Gα_s and Gα_q/11 are activated by the V₂R at the plasma membrane. In cells expressing the early endosomal marker EEA1, robust colocalization between Halo-mGs, Halo-mGsq and RFP-EEA1 were found 45 min after initial AVP stimulation but not by vehicle treatment (Figure 2C, D). Together our EbBRET and confocal microscopy imaging data suggest that Gα_s and Gα_q/11 are activated by V₂R first at plasma membrane, and later on, from early endosomes after the V₂R has been internalized.

G protein activation from endosomes by some GPCRs is associated with the ability of the receptor to recruit G protein and βarr simultaneously to form a GPCR–βarr–G protein megaplex. As we already previously demonstrated that the V₂R forms V₂R-βarr-G_s megaplexes upon AVP stimulation (Thomsen et al., 2016), we here explored whether formation of such complexes potentially can be formed with G_q/11 as well. In addition to the V₂R, we also applied a chimeric V₂R harboring the carboxy-terminal tail of the β₂-adrenergic receptor (β₂AR) referred to as V₂β₂AR. We previously showed that the phosphorylated V₂R carboxy-tail forms stable complexes with βarr, a requirement of megaplex formation, whereas the carboxy-tail of the β₂AR does not (Cahill et al., 2017). Therefore, we expected that only the V₂R, but not the V₂β₂AR, recruits G proteins and βarrs simultaneously upon agonist challenge.

Both the V₂R and V₂β₂AR bind to AVP with similar affinities and activate adenylyl cyclase via Gα_s with similar potencies (Oakley et al., 1999). We monitored activation of the four Gα protein families at the plasma membrane by the V₂β₂AR upon AVP treatment using the same approach utilized in Figure 1A. Similar to the V₂R, the V₂β₂AR activated both Gα_s and Gα_q, but not Gα_i nor Gα₁₂, at plasma membrane with a maximal response reached after ~10 min of stimulation (Figure 3A, Figure 3—figure supplement 1). While the V₂R and V₂β₂AR are both reported to internalize via a βarr-dependent mechanism, βarr has been reported to rapidly dissociate from the V₂β₂AR shortly after its recruitment to the plasma membrane due to its low affinity for this receptor chimera (Oakley et al., 1999). In contrast, βarr stays associated with the V₂R during its internalization into endosomes owning to its high affinity for the V₂R (Oakley et al., 1999). Here, we compared the kinetics of βarr1 and βarr2 recruitment to the V₂R and V₂β₂AR at the plasma membrane and early endosomes by monitoring AVP-promoted EbBRET between Rluc-fused βarrs and rGFP-CAAX or rGFP-Rab5, respectively (Figure 3B, left panel). At similar levels of receptor and βarr expressions (Figure 3—figure supplement 2), both receptors recruited βarr1 and βarr2 at plasma membrane maximally after 10 min of stimulation with AVP (Figure 3B, right upper panel). However, the presence of βarrs at the plasma membrane declined rapidly hereafter 10 min in V₂R-expressing cells, while remaining for longer periods of time in V₂β₂AR-expressing cells. These findings are in line with the previous reported observations (Oakley et al., 1999). Additionally, the translocation of βarrs to the plasma membrane was more robust for the V₂R as compared to the V₂β₂AR, which is reminiscent from the higher affinity of βarrs for the V₂R as compared to theβ₂AR (Oakley et al., 2000). In contrast to the rapid translocation of βarrs to the plasma membrane, AVP treatment induced a robust but slower enrichment of βarrs to early endosomes with V₂R reaching a maximal response after approximately 45 min of stimulation (Figure 3B, right bottom panel). Importantly, as opposed to V₂R, AVP stimulation of V₂β₂AR did not result in βarr translocation to early endosomes (Figure 3B, right bottom panel). Consequently, the V₂β₂AR represents a valuable negative control to investigate the ability to recruit G proteins and βarrs simultaneously at endosomes.

To track the simultaneous coupling of G proteins and βarrs to GPCRs in real time, a nanoBiT approach was used. Both mGs and mGsq were fused to the large portion of nanoluciferase (large-BiT; LgBiT) and βarr1 to an optimized small peptide BiT (small BiT; SmBiT). Reconstitution of the complete and functional nanoluciferase, which catalyzes the conversion of coelenterazine-h, results in emission of a bright luminescence signal. In our setup, close proximity of LgBiT and SmBiT only occurs when LgBiT-mG and SmBiT-βarr1 are recruited simultaneously to the receptor, which is a hallmark of megaplex formation (Figure 3C, left panel). Using this approach, we detected bright luminescence signals involving mGs/βarr1 (Figure 3C, upper right panel) and mGsq/βarr1 (Figure 3C, bottom right panel) upon stimulation of the V₂R but not the V₂β₂AR. Interestingly, the dual coupling of Gα_q/11/βarr to V₂R appeared to be faster than the co-coupling of Gα_s/βarr. While 20 min was required to reach the maximal response of V₂R-stimulated mGs/βarr1 co-coupling, 8 min was sufficient to obtain the maximal levels of mGsq/βarr1 recruitment to the V₂R (Figure 3C, right panels).

To visualize the simultaneous recruitment of G proteins and βarr to the receptors by confocal microscopy, we transfected HEK293 cells with βarr2 fused to strawberry (strawberry-βarr2), Halo-mGs or Halo-mGsq, and the V₂R or V₂β₂AR. In vehicle-treated cells, both mGs and βarr2 were homogenously distributed in the cytosol (Figure 3D, upper panels). However, after prolonged stimulation of V₂R with AVP, around 75% of βarr2 colocalized with mGs in endocytic vesicles (Figure 3D, F). In V₂β₂AR-stimulated cells, little to no colocalization was observed between βarr2 and mGs upon prolonged stimulation with AVP (Figure 3D, F). Surprisingly, however, some clusters of intracellular mGs were visible despite the absence of βarr2 (Figure 3D). As opposed to V₂R-expressing cells for which nearly all the clusters of intracellular mGs colocalize with βarr2, in V₂β₂AR-expressing cells around 75% of the intracellular mGs clusters do not colocalize with βarr2 (Figure 3G). These results suggest simultaneous coupling of Gα_s/βarr2 to the V₂R in endosomes but not to the V₂β₂AR. It is important to specify here that as mG proteins are recruited to receptors in the active conformation, the mG clusters are located where the active receptors are. Consequently, colocalization of mGs with βarr2 necessarily implies the presence of the active receptor in close proximity to both mGs and βarr2. In cells expressing mGsq, both mGsq and βarr2 were also homogenously distributed in the cytosol when cells were treated with the vehicle (Figure 3E, upper panels). Upon AVP stimulation, approximately 75% of βarr2 colocalized with mGsq in intracellular vesicles in V₂R-expressing cells, whereas poor colocalization was observed in V₂β₂AR-expressing cells (Figure 3E, F). Similar to cells expressing mGs, clear clusters of intracellular mGsq were visible in cells expressing V₂β₂AR (Figure 3E, G), suggesting a certain level of endosomal Gα_s/Gα_q signaling despite the absence of βarr2.

Activation of Gα_s and Gα_q/11 by the V₂β₂AR from endosome-like structures in the absence of local βarrs raises the possibility that the V₂R can activate these G proteins from endosomes in both βarr-dependent and -independent manners. To test this hypothesis, we compared AVP-induced Gα_s and Gα_q/11 activation at plasma membrane and endosomes in CRISPR/Cas9-engineered βarr1- and βarr2-deficient HEK293 cells (Δβarr1/2) (Namkung et al., 2016) as well as their parental cellular counterpart. The surface expression of V₂R was matched in both cellular backgrounds (Figure 4—figure supplement 1). Using the EbBRET biosensors described in Figure 1A, B, we performed AVP concentration–response characterization of Gα_s and Gα_q/11 activation at the plasma membrane (Figure 4A) and early endosomes (Figure 4B) in parental and Δβarr1/2 HEK293 cells. In contrast to Gα_s and Gα_q/11 activation at the plasma membrane, which were not negatively affected by the absence of βarrs (Figure 4A, Table 1), we observed a robust decrease in the ability of the V₂R to activate Gα_s and Gα_q at endosomes in Δβarr1/2 cells as compared to their parental counterpart (Figure 4B, Table 1). These data demonstrate the important role of βarrs in endosomal Gα_s/Gα_q/11 activation by the V₂R. However, although the Δβarr1/2 cells do not express βarrs, we still observed significant residual G protein activation from endosomes. This surprising observation suggests that the V₂R internalizes into endosomes to some extent in a βarr-independent manner from where G proteins are stimulated. To probe this possibility, we compared V₂R internalization in parental and Δβarr1/2 HEK293 cells expressing rGFP-CAAX and equivalent amounts of V₂R fused to Rluc at its carboxy-terminal tail (V₂R-Rluc) (Figure 3C, left panel, Figure 4—figure supplement 2). In parental HEK293 cells, AVP stimulation of the V₂R-Rluc led to a robust decrease of EbBRET values, which indicates strong receptor internalization (Figure 4C, right panel). Interestingly, we also observed significant internalization of the V₂R in Δβarr1/2 HEK293 cells, although less than in the parental cells (Figure 4C, right panel). These results suggest that a minor population of V₂R internalizes independently of βarrs and contributes to endosomal Gα_s and Gα_q/11 signaling.

Although our data suggest that a minor population of V₂R internalizes in the absence of βarrs and contribute to V₂R-mediated endosomal Gα_s signaling, it has been reported that βarr binding to the V₂R and PTHR potentiates endosomal Gα_s signaling (Feinstein et al., 2011; Feinstein et al., 2013). To verify this and determine if this potentiator effect of βarrs also affects endosomal Gα_q/11 signaling, we compared endosomal Gα_s and Gα_q/11 activation in cells expressing similar levels of V₂R or V₂β₂AR (Figure 4—figure supplement 3). Our rationale for using these two receptors is that if βarrs potentiate endosomal G protein activation, this potentiator effect will be observed to a greater extent for the V₂R since this receptor associates more robustly with βarrs as compared to the V₂β₂AR. Using the same biosensors as in Figure 1A, B, we performed AVP dose–response curves of mGs and mGsq recruitment to the plasma membrane and early endosomes (Figure 4—figure supplement 4, Table 2). From the dose–response curves obtained (Figure 4—figure supplement 4, Table 2), we determined the transduction coefficient log(τ/Ka), a parameter that combines efficiency and potency to determine the overall G protein transduction, for each condition using the operational model of Kenakin and Christopoulos (Kenakin et al., 2012). In cells expressing mGs, the transduction coefficients of Gα_s activation at the plasma membrane were similar for the V₂R and V₂β₂AR, but higher for the V₂R than V₂β₂AR in early endosomes (Figure 4D, left panel, Table 2). Similarly, in cells expressing mGsq, the transduction coefficients of Gα_q/11 activation at the plasma membrane were similar for the V₂R and V₂β₂AR, but higher for the V₂R than V₂β₂AR in early endosomes (Figure 4D, right panel, Table 2). Altogether these results indicate that βarrs potentiate activation of G proteins by the V₂R in early endosomes.

HEK293 clonal cell line (HEK293SL cells) and referred as HEK293 cells as well as the HEK293 cells devoid of βarr1 and βarr2 referred as Δβarr1/2 cells were a gift from Stephane Laporte (McGill University, Montreal, Quebec, Canada), previously described and authenticated by STR (Namkung et al., 2016). These cells were cultured in Dulbecco’s modified Eagle’s medium high glucose (Life Technologies, Paisley, UK) supplemented with 10% fetal bovine serum and 100 units per ml penicillin–streptomycin (Life Technologies, Paisley, UK), maintained at 37°C and 5% CO₂ and passaged every 3–4 days using trypsin–EDTA = Ethylenediaminetetraacetic acid 0.05% (Life Technologies, Paisley, UK) to detach the cells. All cells were monthly checked for mycoplasma contamination and were negative. DNA to be transfected was combined with salmon sperm DNA (Thermo Fisher Scientific, Cambridge, UK) to obtain a total of 1 µg DNA per condition. Linear polyethyleneimine 25 K (PEI; Polysciences Europe GmbH, Germany) was combined with DNA (3 μg PEI per μg of DNA), vortexed and incubated 20 min before adding a cell suspension containing 300,000 cells per ml (1.2 ml of cells per condition). The appropriate volume of cells containing the DNA was seeded and cells were incubated for 48 hr before assay.

All DNA constructs were cloned into pcDNA3.1(+) expression plasmid except if stated otherwise. V₂R and V₂β₂AR were tagged with a HA epitope in amino-terminal of the receptors. HA-V₂R was synthetized by GenScript and HA-V₂β₂AR was generously provided by Dr Robert Lefkowitz (Duke University, USA). C-tRFP-Lck (cloned into PCMV6-AC-RFP expression vector) and TagRFP-T-EEA1 (cloned into pEGFP-C1 vector) were purchased from Addgene (respectively #RC100049 and #42635). Strawberry-βarr2 was a gift from Prof. Marc G. Caron (Duke University, USA). rGFP-CAAX (Namkung et al., 2016), rGFP-Rab5 (Namkung et al., 2016), V₂R-Rluc (Namkung et al., 2016), Rluc-βarr1 (Zimmerman et al., 2012), Rluc-βarr2 (Quoyer et al., 2013), Rluc-C1b (Wright et al., 2021), and the BRET-based unimolecular PKC sensor (Namkung et al., 2018) were previously described. Rluc-mGs, Rluc-mGsi, Rluc-mGsq, and Rluc-mG12 were synthetized by Twist Bioscience and cloned into pTwistCMV expression vector. The Venus tag in NES-Venus-mGs, NES-Venus-mGsi, NES-Venus-mGsq, and NES-Venus-mG12 previously described (Wan et al., 2018) was replaced by Rluc. Halo-tagged mG constructs was kindly provided by Prof. Nevin A. Lambert (Augusta University, USA). LgBiT-mGsq and SmBiT-βarr1 were synthetized by GenScript. mGsq and βarr1 were tagged in amino-terminal with LgBiT and a linker peptide and SmBiT, respectively.

The cell suspension containing DNA (BRET/EbBRET biosensors and receptors) were seeded in white 96-well plates (Greiner, Stonehouse, UK) at 30,000 cells/well (100 μl per well). Forty-eight hours after transfection, cells were washed with DPBS = Dulbecco's phosphate buffered saline (Life Technologies, Paisley, UK) and assayed in Tyrode’s buffer containing 137 mM NaCl, 0.9 mM KCl, 1 mM MgCl₂, 11.9 mM NaHCO₃, 3.6 mM NaH₂PO₄, 25 mM HEPES = 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, 5.5 mM glucose, 1 mM CaCl₂ (pH 7.4) at 37°C. AVP or vehicle (water) were added and cells incubated at 37°C for the required time. Five minutes before reading, 2.5 μM of the Rluc substrate coelenterazine 400a (NanoLight Technology, Pinetop, AZ, USA) was added. All BRET and EbBRET measurements were performed using a FLUOstar Omega microplate reader (BMG Labtech, Ortenberg, Germany) with an acceptor filter (515 ± 30 nm) and donor filter (410 ± 80 nm). BRET/EbBRET values were determined by calculating the ratio of the light intensity emitted by the acceptor over the light intensity emitted by the donor. ΔEbBRET is defined as the values of EbBRET in presence of AVP (Merck Life Science, Gillingham, UK) minus the value obtained with vehicle. Dose–response curves were fitted using nonlinear regression using a 4-parameter equation and the basal ΔEbBRET was fixed to zero. Statistical significance of parameters of dose–response curves (AVP-induced maximal efficacy or potency) was established by comparing independent fits with a global fit that shares the selected parameter using extra sum-of-squares F-test. The transduction coefficients log(τ/Ka) were determined using the operational from Kenakin and Christopoulos as previously described (van der Westhuizen et al., 2014).

The nanoBiT assay to measure proximity between LgBiT-mG proteins and SmBiT-βarr1 has been reported previously (Hahn et al., 2022). In short, 2,000,000 cells were seeded per well in 6-well plates. Twenty-four hours later, 125 ng SmBiT-βarr1, 1000 ng V₂R or V₂β₂AR, and 125 ng LgBiT-mGs or 1000 ng LgBiT-mGsq were transfected into the cells using Lipofectamine 3000 transfection reagent (Thermo Fisher Scientific, Waltham, MA, USA). The next day, transfected cells were detached and 100,000 cells/well were plated into a Poly-D-lysine-coated white 96-well Microplate (Corning, Corning, NY, USA) and incubated overnight at 37°C. The cells were equilibrated in Opti-MEM (Life Technologies, Paisley, UK) at 37°C for 60 min. Coelenterazine-h (NanoLight Technology, Pinetop, AZ, USA) was added at a final concentration of 10 μM before starting the measurement. After establishing a baseline response for 2 min, cells were stimulated with AVP added at a final concentration of 100 nM and the luminescence was measured for additional 20 min. The signal was detected at 550  nm using a PHERAstar FSX instrument (BMG LabTech, Cary, NC, USA). ΔRLU is defined as the values of relative luminescence in presence of AVP minus the value obtained with vehicle.

Cells containing plasmid DNA encoding for fluorescent-tagged localization markers or strawberry-βarr2, receptors, and mGs, mGsq, or mGsi fused to HaloTag were seeded in 8-well glass chambered slides (Thistle Scientific, Uddingston, Glasgow, UK) at 30,000 cells per well. The day of the assay, mG constructs were labeled by adding HaloTag Oregon Green Ligand (Promega, Chilworth, UK) to cells at a final concentration of 1 μM in the culture media and incubated 15 min (37°C, 5% CO₂). Cells were washed three times with the media and incubated 30 min (37°C, 5% CO₂) for the last wash. The media was aspirated, replaced by Tyrode’s buffer and cells were stimulated with AVP or vehicle (water) for the required time at 37°C, 5% CO₂. At the end of the incubation, the media was aspirated and cells were fixed by adding 300 μl per well of 4% paraformaldehyde in PBS (Life Technologies, Paisley, UK) and incubated at room temperature for 10 min. The paraformaldehyde solution was aspirated, replaced by DPBS and cells were incubated for 10 min before being replaced by Tyrode’s buffer (300 μl per well) and visualized on a SP8 confocal microscope (Leica Biosystems, Nussloch, Germany) at ×63 magnification. Images were quantified using Imaris cell imaging software version 9.9.1 (Oxford Instruments, Abingdon, UK). For each image, a threshold for the red channel was established by selecting the lower intensity from the region of interest (plasma membrane, endosomes, or βarr2). The percentage of colocalization was determined by the percentage of the material from the red channel above threshold colocalized with the material from the green channel. To quantify the percentage of colocalization for confocal microscopy images, the ‘surfaces’ module was selected isolating cells containing the region of interest for each image. Thresholds for each channel were established by selecting the lower intensity from the region of interest (plasma membrane, endosomes, or βarr2). Data are reported as red volume (red voxels) above the threshold that is colocalized with green volume (green voxels) above the threshold and reported as percentage.

To measure the relative cell surface expression of V₂R and V₂β₂AR (both tagged with a HA epitope at their amino-terminal), the same cell suspension containing DNA that was used for EbBRET assays was seeded in white 96-well plates previously coated with Poly-D-lysine (Bio-Techne, Abingdon, UK) at 30,000 cells/well (100 μl per well). Non-transfected cells were used to establish the background of the assay. For the coating, the Poly-D-lysine solution (0.1 mg per ml) was added (50 μl per well) and the plates incubated at 37 ^oC for at least 30 min. Following the incubation, the solution was aspirated and wells washed two times with DPBS before adding the cell suspension containing DNA. Forty-eight hours after seeding, cells were washed with DPBS and fixed by adding 50 μl per well of 4% paraformaldehyde in PBS and incubated at room temperature for 10 min. The fixing solution was aspirated and wells washed three times in a washing buffer composed of DPBS containing 0.5% of bovine serum albumin (Merck Life Science, Gillingham, UK). The washing buffer was left in the wells for 10 min following the last wash. After the 10-min incubation, the buffer was removed and 50 μl per well of monoclonal 3F10 anti-HA-Peroxidase (cat# 12013819001, Merck Life Science, Gillingham, UK) 12.5 ng per ml in washing buffer was added and the plate incubated 1 hr at room temperature. The antibody was aspirated and wells washed three times with the washing buffer. The washing buffer was left in the wells for 10 min following the last wash and wells were washed again three times with DBPS only. After aspiration of the DPBS, 100 μl per well of SigmaFast OPD (Merck Life Science, Gillingham, UK) solution prepared as recommended by the manufacturer was added. Wells were incubated in presence of the OPD solution until the wells containing cells expressing receptors become yellow (typically 10 min). The reaction was stopped by addition of 25 μl per well of hydrochloride 3 M in water. 100 µl per well were transferred to a transparent clear 96-well flat bottom plate (Thermo Fisher Scientific, Cambridge, UK) and absorbance at 492 nm was measured using a FLUOstar Omega microplate reader. The net absorbance represents the absorbance measured in presence of receptor minus the background (i.e. absorbance measured in absence of receptor).

In all experiments at least three independent experiments were performed. n value is provided in the corresponding figure legend. All experiments were performed in quadruplicates. A p-value ≤0.05 was considered as statistically significant for all analyses. Normally distributed and normalized data to control for unwanted sources of variation are shown as mean ± standard error on mean. All statistical analyses and nonlinear regressions were performed using GraphPad Prism 9.4.1 software.

This work was supported by a Wellcome Trust Seed Award (215229/Z/19/Z) and a New Investigator Award from the UKRI Biotechnology and Biological Sciences Research Council (BB/X002578/1) to BP, a research grant from the LEO Foundation (LF18043) and the NIH (1R35GM147088 and 1R21CA243052) to ARBT, a Doctoral Studentship from the Department for the Economy (DfE) Northern Ireland to CD, a Fellowship from the Armagh Tigers Charitable Trust to AW, and a CITI-GENS Horizon2020 Marie Sklodowska-Curie Doctoral Scholarship to AAG.

The DNA plasmids used in this study can be shared upon request to the corresponding authors, except for the plasmids covered by a MTA from Professors Michel Bouvier and Stéphane Laporte.