Header	LI-COR Imager file
Version	1.0.0
Warning	WARNING! This file format is required for LI-COR Applications. DO NOT MODIFY DATA!
Type	_shape
Working Folder	C:\Users\timwa\TMEM127 Paper\Images\
Acq. Image Id	0000554_01
App Name	Image Studio Lite
..	 
Tag Type	Id	Channel	Name	Shape Version	Shape Type	Shape Quant Type	Pixel Area	Selected	Ref X	Ref Y	Rotation	Text Locn	Text Angle
Shape Definition	5	800	00005	LI-COR PAI SHAPE 1.0	100	201	454784	false	194	93	0.0	Lower Center	0.0
Shape Definition	33	800	00033	LI-COR PAI SHAPE 1.0	100	201	451727	false	1372	59	0.0	Lower Center	0.0
Shape Definition	71	800	00071	LI-COR PAI SHAPE 1.0	100	201	213819	false	205	1089	0.0	Lower Center	0.0
Shape Definition	78	800	00078	LI-COR PAI SHAPE 1.0	100	201	189908	false	1364	1078	0.0	Lower Center	0.0
Tag Type	Id	Channel	Name	Shape Version	Shape Type	Shape Quant Type	Pixel Area	Selected	Ref X	Ref Y	Rotation	Text Locn	Text Angle
Shape Definition	1T	700	pRET (R) [175,155]	 	110	0	0	false	1047	148	0.0	Upper Center	0.0
Shape Definition	3T	700	pAKT (R) [60]	 	110	0	0	false	1078	445	0.0	Upper Center	0.0
Shape Definition	4T	700	Mock-KO	 	110	0	0	false	103	53	0.0	Upper Center	0.0
Shape Definition	5T	700	0m	 	110	0	0	false	292	70	0.0	Upper Center	0.0
Shape Definition	6T	700	5m	 	110	0	0	false	389	72	0.0	Upper Center	0.0
Shape Definition	7T	700	15m	 	110	0	0	false	482	69	0.0	Upper Center	0.0
Shape Definition	8T	700	30m	 	110	0	0	false	575	70	0.0	Upper Center	0.0
Shape Definition	9T	700	1h Van +30m 	 	110	0	0	false	630	49	0.0	Upper Center	0.0
Shape Definition	10T	700	1h LOXO +30m	 	110	0	0	false	722	76	0.0	Upper Center	0.0
Shape Definition	11T	700	1h BLU +30m	 	110	0	0	false	829	48	0.0	Upper Center	0.0
Shape Definition	13T	700	B-Actin (M) [43]	 	110	0	0	false	1073	563	0.0	Upper Center	0.0
Shape Definition	14T	700	TMEM127-KO	 	110	0	0	false	1240	42	0.0	Upper Center	0.0
Shape Definition	15T	700	0m	 	110	0	0	false	1743	39	0.0	Upper Center	0.0
Shape Definition	16T	700	5m	 	110	0	0	false	1840	41	0.0	Upper Center	0.0
Shape Definition	17T	700	15m	 	110	0	0	false	1927	38	0.0	Upper Center	0.0
Shape Definition	18T	700	30m	 	110	0	0	false	2030	39	0.0	Upper Center	0.0
Shape Definition	19T	700	1h Van	 	110	0	0	false	1429	42	0.0	Upper Center	0.0
Shape Definition	20T	700	1h LOXO	 	110	0	0	false	1525	43	0.0	Upper Center	0.0
Shape Definition	21T	700	1h BLU	 	110	0	0	false	1630	41	0.0	Upper Center	0.0
Shape Definition	23T	700	pS6 (R) [32]	 	110	0	0	false	1100	1279	0.0	Upper Center	0.0
Shape Definition	25T	700	pERK (M) [44,42]	 	110	0	0	false	1078	1143	0.0	Upper Center	0.0
Tag Type	Id	Channel	Point Pairs
Shape Boundary	5	800	5	194	93	1030	93	1030	637	194	637	194	93
Shape Boundary	33	800	5	1372	59	2183	59	2183	616	1372	616	1372	59
Shape Boundary	71	800	5	205	1089	1018	1089	1018	1352	205	1352	205	1089
Shape Boundary	78	800	5	1364	1078	2152	1078	2152	1319	1364	1319	1364	1078
Tag Type	Id	Channel	Name	Shape Version	Shape Type	Shape Quant Type	Pixel Area	Selected	Ref X	Ref Y	Rotation	Text Locn	Text Angle
Shape Boundary	1T	700	5	1034	122	1234	122	1234	161	1034	161	1034	122
Shape Boundary	3T	700	5	1065	419	1214	419	1214	458	1065	458	1065	419
Shape Boundary	4T	700	5	87	21	224	21	224	69	87	69	87	21
Shape Boundary	5T	700	5	279	44	333	44	333	83	279	83	279	44
Shape Boundary	6T	700	5	376	46	430	46	430	85	376	85	376	46
Shape Boundary	7T	700	5	469	43	534	43	534	82	469	82	469	43
Shape Boundary	8T	700	5	562	44	627	44	627	83	562	83	562	44
Shape Boundary	9T	700	5	617	23	771	23	771	62	617	62	617	23
Shape Boundary	10T	700	5	709	50	878	50	878	89	709	89	709	50
Shape Boundary	11T	700	5	816	22	968	22	968	61	816	61	816	22
Shape Boundary	13T	700	5	1060	537	1226	537	1226	576	1060	576	1060	537
Shape Boundary	14T	700	5	1224	10	1411	10	1411	58	1224	58	1224	10
Shape Boundary	15T	700	5	1730	13	1784	13	1784	52	1730	52	1730	13
Shape Boundary	16T	700	5	1827	15	1881	15	1881	54	1827	54	1827	15
Shape Boundary	17T	700	5	1914	12	1979	12	1979	51	1914	51	1914	12
Shape Boundary	18T	700	5	2017	13	2082	13	2082	52	2017	52	2017	13
Shape Boundary	19T	700	5	1416	16	1507	16	1507	55	1416	55	1416	16
Shape Boundary	20T	700	5	1512	17	1624	17	1624	56	1512	56	1512	17
Shape Boundary	21T	700	5	1617	15	1712	15	1712	54	1617	54	1617	15
Shape Boundary	23T	700	5	1087	1253	1222	1253	1222	1292	1087	1292	1087	1253
Shape Boundary	25T	700	5	1065	1117	1247	1117	1247	1156	1065	1156	1065	1117
Tag Type	Id	Channel	Description
Shape Description	5	800	
Shape Description	33	800	
Shape Description	71	800	
Shape Description	78	800	
Tag Type	Id	Channel	Total Raw Intensity	Mean	Std Dev	Min Intensity	Max Intensity	Max SNR	Signal	Integr Intensity	Auto Shape Backgnd Thresh	Background	Background Std Dev	PerCent Satur	mm Area	Mean SNR	Trim Mean	Trim Std Dev	Trim Background	Trim Backgnd Std Dev	Trim Signal	Median	Trim Median	Conc Standard	Conc Value	Auto Shape StdDev Mult	Auto Shape Search Limit
Shape Quantification	5	800	239917.796875	0.5275422930717468	1.1060211699758713	0.268798828125	25.625	157.85169447935132	76479.79809570312	547.8177604611914	NaN	0.359375	0.16005925741459184	0	3257.5759687783466	1.0506565151371907	0.3924763706117413	0.05044061243830974	0.359375	0.01571156364638755	15053.97373229015	0.378173828125	NaN	NaN	NaN	NaN	0
Shape Quantification	33	800	253621.828125	0.5614493489265442	1.129227605645843	0.037506103515625	73.0	935.4251064435266	88415.02444458008	633.3086892010584	NaN	0.36572265625	0.07764841551014653	0	3235.6789589087043	2.5206783077689736	0.40530652684023377	0.06935373216422579	0.36572265625	0.02062155678237669	17881.10311011453	0.378173828125	NaN	NaN	NaN	NaN	0
Shape Quantification	71	800	112077.25	0.5241687893867493	1.0754426570629148	0.056243896484375	79.25	1458.718025292685	35236.04440307617	252.39254565303023	NaN	0.359375	0.054082162304240365	0	1531.5658336006045	3.04710070279682	0.3786449532181302	0.04636330946030645	0.359375	0.018626612367219235	4120.282127147379	0.36572265625	NaN	NaN	NaN	NaN	0
Shape Quantification	78	800	205632.453125	1.0828003883361816	2.613420814960577	0.268798828125	32.03125	1226.2576289566382	133814.21411132812	958.4989097462154	NaN	0.378173828125	0.025812745563758108	0	1360.2935395237262	27.297620211229706	0.5581219411501421	0.6670177370251643	0.378173828125	0.018249926938292513	34173.58624837869	0.38427734375	NaN	NaN	NaN	NaN	0
Tag Type	Id	Font Name	Font Style	Font Size	R Value	G Value	B Value	Text Type	Arrow Len	ChanName	Color Name
Text Font	1T	Dialog	0	20	1	1	1	500	5	700	Green
Text Font	3T	Dialog	0	20	1	1	1	500	5	700	Green
Text Font	4T	Dialog	1	24	1	1	1	500	5	700	Red
Text Font	5T	Dialog	1	20	1	1	1	500	5	700	BlackWhite
Text Font	6T	Dialog	1	20	1	1	1	500	5	700	BlackWhite
Text Font	7T	Dialog	1	20	1	1	1	500	5	700	BlackWhite
Text Font	8T	Dialog	1	20	1	1	1	500	5	700	BlackWhite
Text Font	9T	Dialog	1	20	1	1	1	500	5	700	BlackWhite
Text Font	10T	Dialog	1	20	1	1	1	500	5	700	BlackWhite
Text Font	11T	Dialog	1	20	1	1	1	500	5	700	BlackWhite
Text Font	13T	Dialog	0	20	1	1	1	500	5	700	Magenta
Text Font	14T	Dialog	1	24	1	1	1	500	5	700	Red
Text Font	15T	Dialog	1	20	1	1	1	500	5	700	BlackWhite
Text Font	16T	Dialog	1	20	1	1	1	500	5	700	BlackWhite
Text Font	17T	Dialog	1	20	1	1	1	500	5	700	BlackWhite
Text Font	18T	Dialog	1	20	1	1	1	500	5	700	BlackWhite
Text Font	19T	Dialog	1	20	1	1	1	500	5	700	BlackWhite
Text Font	20T	Dialog	1	20	1	1	1	500	5	700	BlackWhite
Text Font	21T	Dialog	1	20	1	1	1	500	5	700	BlackWhite
Text Font	23T	Dialog	0	20	1	1	1	500	5	700	Green
Text Font	25T	Dialog	0	20	1	1	1	500	5	700	Magenta
Tag Type	Id	Channel	Peak Location	Size	Lane Name	Lane Channel	Band Name
Tag Type	Id	Font Name	Font Style	Font Size	R Value	G Value	B Value	Text Type	Arrow Len	ChanName	Color Name
Text Font	5	Dialog	1	20	1	1	1	500	5	800	BlackWhite
Text Font	33	Dialog	1	20	1	1	1	500	5	800	BlackWhite
Text Font	71	Dialog	1	20	1	1	1	500	5	800	BlackWhite
Text Font	78	Dialog	1	20	1	1	1	500	5	800	BlackWhite
