Header	LI-COR Imager file
Version	1.0.0
Warning	WARNING! This file format is required for LI-COR Applications. DO NOT MODIFY DATA!
Type	_shape
Working Folder	C:\Users\timwa\TMEM127 Paper\Images\
Acq. Image Id	0001537_01
App Name	Image Studio Lite
..	 
Tag Type	Id	Channel	Name	Shape Version	Shape Type	Shape Quant Type	Pixel Area	Selected	Ref X	Ref Y	Rotation	Text Locn	Text Angle
Shape Definition	1	800	00001	LI-COR PAI SHAPE 1.0	100	201	68651	false	103	100	0.0	Lower Center	0.0
Shape Definition	9	800	00009	LI-COR PAI SHAPE 1.0	100	201	70812	false	1215	101	0.0	Lower Center	0.0
Shape Definition	17	800	00017	LI-COR PAI SHAPE 1.0	100	201	469796	false	71	850	0.0	Lower Center	0.0
Shape Definition	25	800	00025	LI-COR PAI SHAPE 1.0	100	201	493830	false	1206	828	0.0	Lower Center	0.0
Tag Type	Id	Channel	Name	Shape Version	Shape Type	Shape Quant Type	Pixel Area	Selected	Ref X	Ref Y	Rotation	Text Locn	Text Angle
Shape Definition	1T	700	NT	 	110	0	0	false	158	77	0.0	Upper Center	0.0
Shape Definition	2T	700	DMSO	 	110	0	0	false	241	54	0.0	Upper Center	0.0
Shape Definition	3T	700	SELP(uM) +GDNF	 	110	0	0	false	325	53	0.0	Upper Center	0.0
Shape Definition	4T	700	1h Trtment	 	110	0	0	false	20	76	0.0	Upper Center	0.0
Shape Definition	5T	700	+GDNF	 	110	0	0	false	237	84	0.0	Upper Center	0.0
Shape Definition	6T	700	0.1	 	110	0	0	false	353	88	0.0	Upper Center	0.0
Shape Definition	7T	700	0.25	 	110	0	0	false	443	87	0.0	Upper Center	0.0
Shape Definition	8T	700	0.5	 	110	0	0	false	540	86	0.0	Upper Center	0.0
Shape Definition	9T	700	1	 	110	0	0	false	646	85	0.0	Upper Center	0.0
Shape Definition	10T	700	2.5	 	110	0	0	false	734	87	0.0	Upper Center	0.0
Shape Definition	11T	700	5	 	110	0	0	false	846	87	0.0	Upper Center	0.0
Shape Definition	22T	700	Figure 8 Supp2	 	110	0	0	false	974	41	0.0	Upper Center	0.0
Shape Definition	24T	700	pRET [Y905]	 	110	0	0	false	1011	143	0.0	Upper Center	0.0
Shape Definition	25T	700	RET	 	110	0	0	false	1080	920	0.0	Upper Center	0.0
Shape Definition	26T	700	NT	 	110	0	0	false	1273	75	0.0	Upper Center	0.0
Shape Definition	27T	700	DMSO	 	110	0	0	false	1356	52	0.0	Upper Center	0.0
Shape Definition	28T	700	SELP(uM) +GDNF	 	110	0	0	false	1440	51	0.0	Upper Center	0.0
Shape Definition	29T	700	+GDNF	 	110	0	0	false	1352	82	0.0	Upper Center	0.0
Shape Definition	30T	700	0.1	 	110	0	0	false	1468	86	0.0	Upper Center	0.0
Shape Definition	31T	700	0.25	 	110	0	0	false	1558	85	0.0	Upper Center	0.0
Shape Definition	32T	700	0.5	 	110	0	0	false	1655	84	0.0	Upper Center	0.0
Shape Definition	33T	700	1	 	110	0	0	false	1761	83	0.0	Upper Center	0.0
Shape Definition	34T	700	2.5	 	110	0	0	false	1849	85	0.0	Upper Center	0.0
Shape Definition	35T	700	5	 	110	0	0	false	1961	85	0.0	Upper Center	0.0
Shape Definition	36T	700	B-Actin	 	110	0	0	false	1061	1317	0.0	Upper Center	0.0
Shape Definition	37T	700	Mock-KO	 	110	0	0	false	38	724	0.0	Upper Center	0.0
Shape Definition	38T	700	TMEM127-KO	 	110	0	0	false	1930	752	0.0	Upper Center	0.0
Tag Type	Id	Channel	Point Pairs
Shape Boundary	1	800	5	103	100	972	100	972	179	103	179	103	100
Shape Boundary	9	800	5	1215	101	2058	101	2058	185	1215	185	1215	101
Shape Boundary	17	800	5	71	850	985	850	985	1364	71	1364	71	850
Shape Boundary	25	800	5	1206	828	2091	828	2091	1386	1206	1386	1206	828
Tag Type	Id	Channel	Name	Shape Version	Shape Type	Shape Quant Type	Pixel Area	Selected	Ref X	Ref Y	Rotation	Text Locn	Text Angle
Shape Boundary	1T	700	5	145	51	196	51	196	90	145	90	145	51
Shape Boundary	2T	700	5	228	28	314	28	314	67	228	67	228	28
Shape Boundary	3T	700	5	312	27	502	27	502	66	312	66	312	27
Shape Boundary	4T	700	5	7	50	128	50	128	89	7	89	7	50
Shape Boundary	5T	700	5	224	58	317	58	317	97	224	97	224	58
Shape Boundary	6T	700	5	340	62	394	62	394	101	340	101	340	62
Shape Boundary	7T	700	5	430	61	495	61	495	100	430	100	430	61
Shape Boundary	8T	700	5	527	60	581	60	581	99	527	99	527	60
Shape Boundary	9T	700	5	633	59	670	59	670	98	633	98	633	59
Shape Boundary	10T	700	5	721	61	775	61	775	100	721	100	721	61
Shape Boundary	11T	700	5	833	61	870	61	870	100	833	100	833	61
Shape Boundary	22T	700	5	956	4	1196	4	1196	59	956	59	956	4
Shape Boundary	24T	700	5	998	117	1139	117	1139	156	998	156	998	117
Shape Boundary	25T	700	5	1067	894	1132	894	1132	933	1067	933	1067	894
Shape Boundary	26T	700	5	1260	49	1311	49	1311	88	1260	88	1260	49
Shape Boundary	27T	700	5	1343	26	1429	26	1429	65	1343	65	1343	26
Shape Boundary	28T	700	5	1427	25	1617	25	1617	64	1427	64	1427	25
Shape Boundary	29T	700	5	1339	56	1432	56	1432	95	1339	95	1339	56
Shape Boundary	30T	700	5	1455	60	1509	60	1509	99	1455	99	1455	60
Shape Boundary	31T	700	5	1545	59	1610	59	1610	98	1545	98	1545	59
Shape Boundary	32T	700	5	1642	58	1696	58	1696	97	1642	97	1642	58
Shape Boundary	33T	700	5	1748	57	1785	57	1785	96	1748	96	1748	57
Shape Boundary	34T	700	5	1836	59	1890	59	1890	98	1836	98	1836	59
Shape Boundary	35T	700	5	1948	59	1985	59	1985	98	1948	98	1948	59
Shape Boundary	36T	700	5	1048	1291	1137	1291	1137	1330	1048	1330	1048	1291
Shape Boundary	37T	700	5	20	687	180	687	180	742	20	742	20	687
Shape Boundary	38T	700	5	1912	715	2133	715	2133	770	1912	770	1912	715
Tag Type	Id	Channel	Description
Shape Description	1	800	
Shape Description	9	800	
Shape Description	17	800	
Shape Description	25	800	
Tag Type	Id	Channel	Total Raw Intensity	Mean	Std Dev	Min Intensity	Max Intensity	Max SNR	Signal	Integr Intensity	Auto Shape Backgnd Thresh	Background	Background Std Dev	PerCent Satur	mm Area	Mean SNR	Trim Mean	Trim Std Dev	Trim Background	Trim Backgnd Std Dev	Trim Signal	Median	Trim Median	Conc Standard	Conc Value	Auto Shape StdDev Mult	Auto Shape Search Limit
Shape Quantification	1	800	25882.32421875	0.37701305747032166	0.18146210628953657	0.28125	2.97265625	78.62356975968183	2501.42919921875	17.92302176140146	NaN	0.340576171875	0.03347698516068563	0	491.8921420307488	1.0884162258981864	0.3502238695285821	0.019508148237169145	0.340576171875	0.013342396345464354	662.3240916160639	0.346923828125	NaN	NaN	NaN	NaN	0
Shape Quantification	9	800	30323.091796875	0.42821967601776123	0.3053398770730718	0.2406005859375	4.39453125	85.89784674418422	5324.5201416015625	38.15078611729266	NaN	0.35302734375	0.04705011894286667	0	507.3759502626529	1.5981328840485627	0.3743268779567172	0.045468903789565585	0.35302734375	0.014708189476572573	1508.262616246059	0.359375	NaN	NaN	NaN	NaN	0
Shape Quantification	17	800	334489.3125	0.7119884490966797	1.389119273467155	0.1375732421875	19.640625	287.73272714937013	159806.87731933594	1145.0342630967245	NaN	0.371826171875	0.06696769956975393	0	3366.1412180081525	5.07949772327839	0.4367552116700108	0.18939472077631112	0.371826171875	0.032226071903118395	30503.403179536894	0.371826171875	NaN	NaN	NaN	NaN	0
Shape Quantification	25	800	702743.375	1.4230471849441528	4.246434237554374	0.253173828125	58.90625	323.9052507183738	519124.4729003906	3719.5852785182524	NaN	0.371826171875	0.18071464941770574	0	3538.3475331611294	5.817021566816437	0.5832859011566384	0.6094854723603902	0.371826171875	0.050761241321154456	104425.15811115147	0.378173828125	NaN	NaN	NaN	NaN	0
Tag Type	Id	Font Name	Font Style	Font Size	R Value	G Value	B Value	Text Type	Arrow Len	ChanName	Color Name
Text Font	1T	Dialog	0	20	1	1	1	500	5	700	BlackWhite
Text Font	2T	Dialog	0	20	1	1	1	500	5	700	BlackWhite
Text Font	3T	Dialog	0	20	1	1	1	500	5	700	BlackWhite
Text Font	4T	Dialog	0	20	1	1	1	500	5	700	Purple
Text Font	5T	Dialog	0	20	1	1	1	500	5	700	BlackWhite
Text Font	6T	Dialog	0	20	1	1	1	500	5	700	BlackWhite
Text Font	7T	Dialog	0	20	1	1	1	500	5	700	BlackWhite
Text Font	8T	Dialog	0	20	1	1	1	500	5	700	BlackWhite
Text Font	9T	Dialog	0	20	1	1	1	500	5	700	BlackWhite
Text Font	10T	Dialog	0	20	1	1	1	500	5	700	BlackWhite
Text Font	11T	Dialog	0	20	1	1	1	500	5	700	BlackWhite
Text Font	22T	Dialog	1	28	1	1	1	500	5	700	Red
Text Font	24T	Dialog	0	20	1	1	1	500	5	700	Green
Text Font	25T	Dialog	0	20	1	1	1	500	5	700	Green
Text Font	26T	Dialog	0	20	1	1	1	500	5	700	BlackWhite
Text Font	27T	Dialog	0	20	1	1	1	500	5	700	BlackWhite
Text Font	28T	Dialog	0	20	1	1	1	500	5	700	BlackWhite
Text Font	29T	Dialog	0	20	1	1	1	500	5	700	BlackWhite
Text Font	30T	Dialog	0	20	1	1	1	500	5	700	BlackWhite
Text Font	31T	Dialog	0	20	1	1	1	500	5	700	BlackWhite
Text Font	32T	Dialog	0	20	1	1	1	500	5	700	BlackWhite
Text Font	33T	Dialog	0	20	1	1	1	500	5	700	BlackWhite
Text Font	34T	Dialog	0	20	1	1	1	500	5	700	BlackWhite
Text Font	35T	Dialog	0	20	1	1	1	500	5	700	BlackWhite
Text Font	36T	Dialog	0	20	1	1	1	500	5	700	Magenta
Text Font	37T	Dialog	1	28	1	1	1	500	5	700	LightBlue
Text Font	38T	Dialog	1	28	1	1	1	500	5	700	LightBlue
Tag Type	Id	Channel	Peak Location	Size	Lane Name	Lane Channel	Band Name
Tag Type	Id	Font Name	Font Style	Font Size	R Value	G Value	B Value	Text Type	Arrow Len	ChanName	Color Name
Text Font	1	Dialog	1	20	1	1	1	500	5	800	BlackWhite
Text Font	9	Dialog	1	20	1	1	1	500	5	800	BlackWhite
Text Font	17	Dialog	1	20	1	1	1	500	5	800	BlackWhite
Text Font	25	Dialog	1	20	1	1	1	500	5	800	BlackWhite
