Table Analyzed IVF COC Embryo development development Control C1/C3 DKO "4/8 cell embryos" 100 57,1 100 100 100 100 75 75 44,4 100 80 100 66,7 100 Morulas 100 57,1 100 100 100 100 75 62,5 44,4 100 80 78,6 16,7 50 Blastocysts 87,5 50 50 50 100 95,5 100 75 37,5 44,4 75 80 64,3 16,7 40 0 Mixed-effects model (REML) Matching: Across row Assume sphericity? Yes Alpha 0,05 Fixed effects (type III) P value P value summary Statistically significant (P < 0,05)? F (DFn, DFd) Row Factor 0,0231 * Yes F (2, 21) = 4,530 Column Factor 0,0035 ** Yes F (1, 17) = 11,52 Row Factor x Column Factor 0,3920 ns No F (2, 17) = 0,9901 Random effects SD Variance Subject 4,074 16,60 Residual 22,53 507,7 Was the matching effective? Chi-square, df 0,01889, 1 P value 0,8907 P value summary ns Is there significant matching (P < 0.05)? No Difference between column means Predicted mean of Control 85,54 Predicted mean of C1/C3 DKO 62,40 Difference between predicted means 23,14 SE of difference 6,818 95% CI of difference 8,756 to 37,52 Data summary Number of columns (Column Factor) 2 Number of rows (Row Factor) 3 Number of subjects (Subject) 24 Number of missing values 4 Compare each cell mean with the other cell mean in that row Number of families 1 Number of comparisons per family 3 Alpha 0,05 Uncorrected Fisher's LSD Predicted (LS) mean diff, 95,00% CI of diff, Significant? Summary Individual P Value Control - C1/C3 DKO Row 1 9,585 -15,88 to 35,05 No ns 0,4381 Morulas 28,57 3,105 to 54,04 Yes * 0,0301 Blastocysts 31,26 7,493 to 55,03 Yes * 0,0130 Test details Predicted (LS) mean 1 Predicted (LS) mean 2 Predicted (LS) mean diff, SE of diff, N1 N2 t DF Control - C1/C3 DKO Row 1 90,39 80,80 9,585 12,07 7 7 0,7940 17,00 Morulas 90,25 61,67 28,57 12,07 7 7 2,367 17,00 Blastocysts 76,00 44,74 31,26 11,27 8 8 2,775 17,00