Table Analyzed Ca2+ Control C1/C3 DKO Fresh 0,8369995 0,7477449 0,6876253 0,6201364 0,6851183 0,7461624 0,8971356 0,8362415 1,025271 1,307361 CAP 1,112676 1,235322 1,215102 0,9373245 1,033494 1,401488 1,279316 1,719815 1,29653 1,40012 Two-way RM ANOVA Matching: Stacked Assume sphericity? Yes Alpha 0,05 Source of Variation % of total variation P value P value summary Significant? Interaction 0,3178 0,6702 ns No Time 52,88 0,0005 *** Yes Column Factor 23,01 0,0033 ** Yes Subject 10,77 0,6020 ns No ANOVA table SS DF MS F (DFn, DFd) P value Interaction 0,005406 1 0,005406 F (1, 8) = 0,1954 P=0,6702 Time 0,8995 1 0,8995 F (1, 8) = 32,52 P=0,0005 Column Factor 0,3914 1 0,3914 F (1, 8) = 17,09 P=0,0033 Subject 0,1832 8 0,02290 F (8, 8) = 0,8280 P=0,6020 Residual 0,2213 8 0,02766 Difference between row means Mean of Fresh 0,8390 Mean of CAP 1,263 Difference between means -0,4241 SE of difference 0,07438 95% CI of difference -0,5957 to -0,2526 Difference between column means Mean of Control 0,9112 Mean of C1/C3 DKO 1,191 Difference between means -0,2798 SE of difference 0,06768 95% CI of difference -0,4359 to -0,1237 Interaction CI Mean diff, A1 - B1 -0,2469 Mean diff, A2 - B2 -0,3127 (A1 -B1) - (A2 - B2) 0,06576 95% CI of difference -0,2773 to 0,4088 (B1 - A1) - (B2 - A2) -0,06576 95% CI of difference -0,4088 to 0,2773 Data summary Number of columns (Column Factor) 2 Number of rows (Time) 2 Number of subjects (Subject) 10 Compare each cell mean with the other cell mean in that row Number of families 1 Number of comparisons per family 2 Alpha 0,05 Holm-Sidak's multiple comparisons test Mean Diff, Significant? Summary Adjusted P Value Control - C1/C3 DKO Fresh -0,2469 Yes * 0,0259 CAP -0,3127 Yes * 0,0135 Test details Mean 1 Mean 2 Mean Diff, SE of diff, N1 N2 t DF Control - C1/C3 DKO Fresh 0,7155 0,9624 -0,2469 0,1006 5 5 2,455 16,00 CAP 1,107 1,419 -0,3127 0,1006 5 5 3,109 16,00